Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal Metastases
Objective. Aimed to study the effects of endostar and cisplatin using an in vivo imaging system (IVIS) in a model of peritoneal metastasis of gastric cancer. Methods. NUGC-4 gastric cancer cells transfected with luciferase gene (NUGC-4-Luc) were injected i.p. into nude mice. One week later, mice wer...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2017/2920384 |
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| author | Lin Jia Shuguang Ren Tao Li Jianing Wu Xinliang Zhou Yan Zhang Jianhua Wu Wei Liu |
| author_facet | Lin Jia Shuguang Ren Tao Li Jianing Wu Xinliang Zhou Yan Zhang Jianhua Wu Wei Liu |
| author_sort | Lin Jia |
| collection | DOAJ |
| description | Objective. Aimed to study the effects of endostar and cisplatin using an in vivo imaging system (IVIS) in a model of peritoneal metastasis of gastric cancer. Methods. NUGC-4 gastric cancer cells transfected with luciferase gene (NUGC-4-Luc) were injected i.p. into nude mice. One week later, mice were randomly injected i.p.: group 1, cisplatin (d1–3) + endostar (d4–7); group 2, endostar (d1–4) + cisplatin (d5–7); group 3, endostar + cisplatin d1, 4, and 7; group 4, saline for two weeks. One week after the final administration, mice were sacrificed. Bioluminescent data, microvessel density (MVD), and lymphatic vessel density (LVD) were analyzed. Results. Among the four groups, there were no significant differences in the weights and in the number of cancer cell photons on days 1 and 8 (P>0.05). On day 15, the numbers in groups 3 and 1 were less than that in group 2 (P<0.05). On day 21, group 3 was significantly less than group 2 (P<0.05). MVD of group 4 was less than that of groups 1 and 2 (P<0.01). There was no significant difference between groups 2 and 3 (P>0.05) or in LVD number among the four groups (P>0.05). Conclusions. IVIS® was more useful than weight, volume of ascites, and number of peritoneal nodules. The simultaneous group was superior to sequential groups in killing cancer cells and inhibiting vascular endothelium. Cisplatin-endostar was superior to endostar-cisplatin in killing cancer cells, while the latter in inhibiting peritoneal vascular endothelium. |
| format | Article |
| id | doaj-art-1fe4ef93cb7d4820bb0f824738212201 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-1fe4ef93cb7d4820bb0f8247382122012025-08-20T03:54:43ZengWileyGastroenterology Research and Practice1687-61211687-630X2017-01-01201710.1155/2017/29203842920384Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal MetastasesLin Jia0Shuguang Ren1Tao Li2Jianing Wu3Xinliang Zhou4Yan Zhang5Jianhua Wu6Wei Liu7Department of Medical Oncology, The Affiliated Hospital of Hebei University, Baoding 071000, ChinaDepartment of Animal Center, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050017, ChinaDepartment of Epidemiology and Health Statistics, School of Public Health, Hebei Medical University, Shijiazhuang 050011, ChinaHebei Province China-Japan Friendship Center for Cancer Detection, Shijiazhuang 050017, ChinaDepartment of Medical Oncology, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050017, ChinaHebei Province China-Japan Friendship Center for Cancer Detection, Shijiazhuang 050017, ChinaDepartment of Animal Center, The Fourth Hospital, Hebei Medical University, Shijiazhuang 050017, ChinaDepartment of Palliative Care Center, Beijing Cancer Hospital, Beijing 100000, ChinaObjective. Aimed to study the effects of endostar and cisplatin using an in vivo imaging system (IVIS) in a model of peritoneal metastasis of gastric cancer. Methods. NUGC-4 gastric cancer cells transfected with luciferase gene (NUGC-4-Luc) were injected i.p. into nude mice. One week later, mice were randomly injected i.p.: group 1, cisplatin (d1–3) + endostar (d4–7); group 2, endostar (d1–4) + cisplatin (d5–7); group 3, endostar + cisplatin d1, 4, and 7; group 4, saline for two weeks. One week after the final administration, mice were sacrificed. Bioluminescent data, microvessel density (MVD), and lymphatic vessel density (LVD) were analyzed. Results. Among the four groups, there were no significant differences in the weights and in the number of cancer cell photons on days 1 and 8 (P>0.05). On day 15, the numbers in groups 3 and 1 were less than that in group 2 (P<0.05). On day 21, group 3 was significantly less than group 2 (P<0.05). MVD of group 4 was less than that of groups 1 and 2 (P<0.01). There was no significant difference between groups 2 and 3 (P>0.05) or in LVD number among the four groups (P>0.05). Conclusions. IVIS® was more useful than weight, volume of ascites, and number of peritoneal nodules. The simultaneous group was superior to sequential groups in killing cancer cells and inhibiting vascular endothelium. Cisplatin-endostar was superior to endostar-cisplatin in killing cancer cells, while the latter in inhibiting peritoneal vascular endothelium.http://dx.doi.org/10.1155/2017/2920384 |
| spellingShingle | Lin Jia Shuguang Ren Tao Li Jianing Wu Xinliang Zhou Yan Zhang Jianhua Wu Wei Liu Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal Metastases Gastroenterology Research and Practice |
| title | Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal Metastases |
| title_full | Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal Metastases |
| title_fullStr | Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal Metastases |
| title_full_unstemmed | Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal Metastases |
| title_short | Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal Metastases |
| title_sort | effects of combined simultaneous and sequential endostar and cisplatin treatment in a mice model of gastric cancer peritoneal metastases |
| url | http://dx.doi.org/10.1155/2017/2920384 |
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