The Role of Uron and Chlorobenzene Derivatives, as Potential Endocrine Disrupting Compounds, in the Secretion of ACTH and PRL
Uron herbicides polluting the environment represent a serious concern for environmental health and may be regarded as endocrine-disrupting compounds (EDCs), which influence the regulation of human homeostasis. We aimed to investigate the effect of EDC urons (phenuron: PU, monuron: MU, and diuron: DU...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2018/7493418 |
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| author | Krisztian Sepp Anna M. Laszlo Zsolt Molnar Andrea Serester Tunde Alapi Marta Galfi Zsuzsanna Valkusz Marianna Radacs |
| author_facet | Krisztian Sepp Anna M. Laszlo Zsolt Molnar Andrea Serester Tunde Alapi Marta Galfi Zsuzsanna Valkusz Marianna Radacs |
| author_sort | Krisztian Sepp |
| collection | DOAJ |
| description | Uron herbicides polluting the environment represent a serious concern for environmental health and may be regarded as endocrine-disrupting compounds (EDCs), which influence the regulation of human homeostasis. We aimed to investigate the effect of EDC urons (phenuron: PU, monuron: MU, and diuron: DU) and chlorobenzenes on the basal release of the adrenocorticotropic hormone (ACTH), which is a part of the adenohypophysis-adrenocortical axis. Hormone secretion in the presence of EDC was studied in two cell types: normal adenohypophysis cells (AdH) and cells of prolactinomas (PRLOMA). PRLOMA was induced in female Wistar rats by subcutaneously injecting them with estrone acetate for 6 months. AdH and PRLOMA were separated from treated and untreated experimental animals, dissociated enzymatically and mechanically in order to create monolayer cell cultures, which served as an experimental in vitro model. We investigated the effects of ED agents separately and in combination on ACTH and prolactin (PRL) release through the hypophyseal-adrenal axis. Hormone determination was carried out by the luminescent immunoassay and the radioimmunoassay methods. Our results showed that (1) uron agents separately did not change ACTH and PRL release in AdH culture; (2) ACTH secretion in arginine vasopressin- (AVP-) activated AdH cells was significantly increased by EDC treatment; (3) ED agents increased the basal hormone release (ACTH, PRL) in PRLOMA cells; and (4) EDC exposure increased ACTH release in AVP-activated PRLOMA cells. We conclude that the herbicides PU, MU, and DU carry EDC effects and show human toxicity potential. |
| format | Article |
| id | doaj-art-1fdaed93a081407fa4d83f14b0db1643 |
| institution | OA Journals |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-1fdaed93a081407fa4d83f14b0db16432025-08-20T02:03:47ZengWileyInternational Journal of Endocrinology1687-83371687-83452018-01-01201810.1155/2018/74934187493418The Role of Uron and Chlorobenzene Derivatives, as Potential Endocrine Disrupting Compounds, in the Secretion of ACTH and PRLKrisztian Sepp0Anna M. Laszlo1Zsolt Molnar2Andrea Serester3Tunde Alapi4Marta Galfi5Zsuzsanna Valkusz6Marianna Radacs7First Department of Medicine, Faculty of Medicine, University of Szeged, Szeged, HungaryDepartment of Biometrics and Agricultural Informatics, Faculty of Horticultural Science, Szent István University, Budapest, HungaryDepartment of Environmental Biology and Education, Institute of Environmental and Technological Sciences, Juhász Gyula Faculty of Education, University of Szeged, Szeged, HungaryDepartment of Environmental Biology and Education, Institute of Environmental and Technological Sciences, Juhász Gyula Faculty of Education, University of Szeged, Szeged, HungaryDepartment of Inorganic and Analytical Chemistry, Faculty of Science and Informatics, University of Szeged, Szeged, HungaryDepartment of Environmental Biology and Education, Institute of Environmental and Technological Sciences, Juhász Gyula Faculty of Education, University of Szeged, Szeged, HungaryFirst Department of Medicine, Faculty of Medicine, University of Szeged, Szeged, HungaryDepartment of Environmental Biology and Education, Institute of Environmental and Technological Sciences, Juhász Gyula Faculty of Education, University of Szeged, Szeged, HungaryUron herbicides polluting the environment represent a serious concern for environmental health and may be regarded as endocrine-disrupting compounds (EDCs), which influence the regulation of human homeostasis. We aimed to investigate the effect of EDC urons (phenuron: PU, monuron: MU, and diuron: DU) and chlorobenzenes on the basal release of the adrenocorticotropic hormone (ACTH), which is a part of the adenohypophysis-adrenocortical axis. Hormone secretion in the presence of EDC was studied in two cell types: normal adenohypophysis cells (AdH) and cells of prolactinomas (PRLOMA). PRLOMA was induced in female Wistar rats by subcutaneously injecting them with estrone acetate for 6 months. AdH and PRLOMA were separated from treated and untreated experimental animals, dissociated enzymatically and mechanically in order to create monolayer cell cultures, which served as an experimental in vitro model. We investigated the effects of ED agents separately and in combination on ACTH and prolactin (PRL) release through the hypophyseal-adrenal axis. Hormone determination was carried out by the luminescent immunoassay and the radioimmunoassay methods. Our results showed that (1) uron agents separately did not change ACTH and PRL release in AdH culture; (2) ACTH secretion in arginine vasopressin- (AVP-) activated AdH cells was significantly increased by EDC treatment; (3) ED agents increased the basal hormone release (ACTH, PRL) in PRLOMA cells; and (4) EDC exposure increased ACTH release in AVP-activated PRLOMA cells. We conclude that the herbicides PU, MU, and DU carry EDC effects and show human toxicity potential.http://dx.doi.org/10.1155/2018/7493418 |
| spellingShingle | Krisztian Sepp Anna M. Laszlo Zsolt Molnar Andrea Serester Tunde Alapi Marta Galfi Zsuzsanna Valkusz Marianna Radacs The Role of Uron and Chlorobenzene Derivatives, as Potential Endocrine Disrupting Compounds, in the Secretion of ACTH and PRL International Journal of Endocrinology |
| title | The Role of Uron and Chlorobenzene Derivatives, as Potential Endocrine Disrupting Compounds, in the Secretion of ACTH and PRL |
| title_full | The Role of Uron and Chlorobenzene Derivatives, as Potential Endocrine Disrupting Compounds, in the Secretion of ACTH and PRL |
| title_fullStr | The Role of Uron and Chlorobenzene Derivatives, as Potential Endocrine Disrupting Compounds, in the Secretion of ACTH and PRL |
| title_full_unstemmed | The Role of Uron and Chlorobenzene Derivatives, as Potential Endocrine Disrupting Compounds, in the Secretion of ACTH and PRL |
| title_short | The Role of Uron and Chlorobenzene Derivatives, as Potential Endocrine Disrupting Compounds, in the Secretion of ACTH and PRL |
| title_sort | role of uron and chlorobenzene derivatives as potential endocrine disrupting compounds in the secretion of acth and prl |
| url | http://dx.doi.org/10.1155/2018/7493418 |
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