RETRACTED ARTICLE: A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticles
Abstract In this study, a novel nanobiocomposite was synthesized using graphene oxide, lignin, silk fibroin and ZnO and used in biological fields. To synthesize this structure, after preparing graphene oxide by the Hummer method, lignin, silk fibroin, and ZnO nanoparticles (NPs) were added to it, re...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-12283-5 |
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| author | Reza Eivazzadeh-Keihan Ensiye Zare-Bakheir Hooman Aghamirza Moghim Aliabadi Mostafa Ghafori Gorab Hossein Ghafuri Ali Maleki Hamid Madanchi Mohammad Mahdavi |
| author_facet | Reza Eivazzadeh-Keihan Ensiye Zare-Bakheir Hooman Aghamirza Moghim Aliabadi Mostafa Ghafori Gorab Hossein Ghafuri Ali Maleki Hamid Madanchi Mohammad Mahdavi |
| author_sort | Reza Eivazzadeh-Keihan |
| collection | DOAJ |
| description | Abstract In this study, a novel nanobiocomposite was synthesized using graphene oxide, lignin, silk fibroin and ZnO and used in biological fields. To synthesize this structure, after preparing graphene oxide by the Hummer method, lignin, silk fibroin, and ZnO nanoparticles (NPs) were added to it, respectively. Also, ZnO NPs with a particle size of about 18 nm to 33 nm was synthesized via Camellia sinensis extract by green methodology. The synthesized structure was examined as anti-biofilm agent and it was observed that the Graphene oxide-lignin/silk fibroin/ZnO nanobiocomposite has a significant ability to prevent the formation of P. aeruginosa biofilm. In addition, due to the importance of the possibility of using this structure in biological environments, its toxicity and blood compatibility were also evaluated. According to the obtained results from MTT assay, the viability percentages of Hu02 cells treated with Graphene oxide-lignin/silk fibroin/ZnO nanobiocomposite after 24, 48, and 72 h of incubation were 89.96%, 89.32%, and 91.28%. On the other hand, the hemolysis percentage of the synthesized structure after 24 h and 72 h of extraction was 9.5% and 11.76% respectively. As a result, the synthesized structure has a hemolysis percentage below 12% and its toxicity effect on Hu02 cells is below 9%. |
| format | Article |
| id | doaj-art-1fda116daf274e38bd4d302fa512887c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-1fda116daf274e38bd4d302fa512887c2025-02-09T12:38:26ZengNature PortfolioScientific Reports2045-23222022-05-0112111210.1038/s41598-022-12283-5RETRACTED ARTICLE: A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticlesReza Eivazzadeh-Keihan0Ensiye Zare-Bakheir1Hooman Aghamirza Moghim Aliabadi2Mostafa Ghafori Gorab3Hossein Ghafuri4Ali Maleki5Hamid Madanchi6Mohammad Mahdavi7Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and TechnologyCatalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and TechnologyProtein Chemistry Laboratory, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of IranCatalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and TechnologyCatalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and TechnologyCatalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and TechnologyDepartment of Biotechnology, School of Medicine, Semnan University of Medical SciencesEndocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical SciencesAbstract In this study, a novel nanobiocomposite was synthesized using graphene oxide, lignin, silk fibroin and ZnO and used in biological fields. To synthesize this structure, after preparing graphene oxide by the Hummer method, lignin, silk fibroin, and ZnO nanoparticles (NPs) were added to it, respectively. Also, ZnO NPs with a particle size of about 18 nm to 33 nm was synthesized via Camellia sinensis extract by green methodology. The synthesized structure was examined as anti-biofilm agent and it was observed that the Graphene oxide-lignin/silk fibroin/ZnO nanobiocomposite has a significant ability to prevent the formation of P. aeruginosa biofilm. In addition, due to the importance of the possibility of using this structure in biological environments, its toxicity and blood compatibility were also evaluated. According to the obtained results from MTT assay, the viability percentages of Hu02 cells treated with Graphene oxide-lignin/silk fibroin/ZnO nanobiocomposite after 24, 48, and 72 h of incubation were 89.96%, 89.32%, and 91.28%. On the other hand, the hemolysis percentage of the synthesized structure after 24 h and 72 h of extraction was 9.5% and 11.76% respectively. As a result, the synthesized structure has a hemolysis percentage below 12% and its toxicity effect on Hu02 cells is below 9%.https://doi.org/10.1038/s41598-022-12283-5 |
| spellingShingle | Reza Eivazzadeh-Keihan Ensiye Zare-Bakheir Hooman Aghamirza Moghim Aliabadi Mostafa Ghafori Gorab Hossein Ghafuri Ali Maleki Hamid Madanchi Mohammad Mahdavi RETRACTED ARTICLE: A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticles Scientific Reports |
| title | RETRACTED ARTICLE: A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticles |
| title_full | RETRACTED ARTICLE: A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticles |
| title_fullStr | RETRACTED ARTICLE: A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticles |
| title_full_unstemmed | RETRACTED ARTICLE: A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticles |
| title_short | RETRACTED ARTICLE: A novel, bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin, silk fibroin and ZnO nanoparticles |
| title_sort | retracted article a novel bioactive and antibacterial scaffold based on functionalized graphene oxide with lignin silk fibroin and zno nanoparticles |
| url | https://doi.org/10.1038/s41598-022-12283-5 |
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