A CRISPR Cas12a/Cpf1 strategy to facilitate robust multiplex gene editing in Aspergillus Niger

A CRISPR Cas12a/Cpf1 strategy to facilitate robust multiplex gene editing in Aspergillus Niger

Abstract Background CRISPR technologies have revolutionized strain engineering of Aspergillus species, and drastically increased the ease and speed at which genomic modifications can be performed. One of the advantages of CRISPR technologies is the possibility of rapid strain engineering using multi...

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Bibliographic Details
Main Authors: Abel Peter van Esch, Samuel Mathew Maurice Prudence, Fabiano Jares Contesini, Bernd Gerhartz, Kate Elizabeth Royle, Uffe Hasbro Mortensen
Format: Article
Language:English
Published: BMC 2025-04-01
Series:Fungal Biology and Biotechnology
Subjects:
CRISPR gene editing
Cpf1
Cas12a
Aspergillus Niger
Multiplexing
Fungi
Online Access:https://doi.org/10.1186/s40694-025-00196-7
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