Identification and antibiogram of clinically relevant non-diphtheriae Corynebacterium species
Background: With the increase in immunosuppressed patients and antimicrobial misuse, Non-diphtheriae Corynebacterium species (NDC spp.) have risen as opportunistic pathogens in hospitalized patients. The current work aimed at comparing Vitek 2C automated identification cards versus MALDI-TOF (Matri...
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Zagazig University, Faculty of Medicine
2024-05-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_339747_497d7d39c2eae5f4635cb8ef36e9aca9.pdf |
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| author | Nada Elwalaly Manal Abd El Alim. sally saber Sherin El Masry Noha Fahim |
| author_facet | Nada Elwalaly Manal Abd El Alim. sally saber Sherin El Masry Noha Fahim |
| author_sort | Nada Elwalaly |
| collection | DOAJ |
| description | Background: With the increase in immunosuppressed patients and antimicrobial misuse, Non-diphtheriae Corynebacterium species (NDC spp.) have risen as opportunistic pathogens in hospitalized patients. The current work aimed at comparing Vitek 2C automated identification cards versus MALDI-TOF (Matrix-associated laser-induced desorption ionization-time of flight) as the gold standard method for the identification of different NDC spp. and determining the antimicrobial susceptibility of NDC spp. by the disc diffusion method for proper management of patients. Methods: Thirty NDC spp. isolates were subjected to identification by MALDI-TOF MS and Vitek 2C and antibiotic susceptibility testing by the disc diffusion method. Results: Our study showed that Corynebacterium (C.) striatum was the most commonly isolated species (83.3%), followed by C. amycolatum (10%). 36.7 % of the isolates were recovered from blood culture while 20 % were recovered from deep wounds. As regard isolate identification by Vitek-2C ANC ID CARD compared to MALDI-TOF as the gold standard method, the agreement was 86.7%. Regarding the antimicrobial susceptibility by disc diffusion method, all the isolates showed increased resistance against penicillin (100%), ciprofloxacin (70%), gentamicin (93.3%), tetracycline (76.6%), and clindamycin (70%). On the other hand, vancomycin and linezolid showed promising results where 100 % of the isolates were susceptible. Conclusion: We spotlight that NDC spp. is not viewed as a contaminant organism anymore, particularly in immunosuppressed patients. Timely identification, proper clinical correlation, and appropriate therapeutic intervention can lead to favorable outcomes. |
| format | Article |
| id | doaj-art-1fcb01d642be4a338d38022d6b9f7070 |
| institution | Kabale University |
| issn | 2682-4132 2682-4140 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj-art-1fcb01d642be4a338d38022d6b9f70702024-12-21T21:02:47ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-05-015268268910.21608/mid.2024.257735.1731339747Identification and antibiogram of clinically relevant non-diphtheriae Corynebacterium speciesNada Elwalaly0Manal Abd El Alim.1sally saber2Sherin El Masry3Noha Fahim4Clinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptClinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptClinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptClinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptClinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptBackground: With the increase in immunosuppressed patients and antimicrobial misuse, Non-diphtheriae Corynebacterium species (NDC spp.) have risen as opportunistic pathogens in hospitalized patients. The current work aimed at comparing Vitek 2C automated identification cards versus MALDI-TOF (Matrix-associated laser-induced desorption ionization-time of flight) as the gold standard method for the identification of different NDC spp. and determining the antimicrobial susceptibility of NDC spp. by the disc diffusion method for proper management of patients. Methods: Thirty NDC spp. isolates were subjected to identification by MALDI-TOF MS and Vitek 2C and antibiotic susceptibility testing by the disc diffusion method. Results: Our study showed that Corynebacterium (C.) striatum was the most commonly isolated species (83.3%), followed by C. amycolatum (10%). 36.7 % of the isolates were recovered from blood culture while 20 % were recovered from deep wounds. As regard isolate identification by Vitek-2C ANC ID CARD compared to MALDI-TOF as the gold standard method, the agreement was 86.7%. Regarding the antimicrobial susceptibility by disc diffusion method, all the isolates showed increased resistance against penicillin (100%), ciprofloxacin (70%), gentamicin (93.3%), tetracycline (76.6%), and clindamycin (70%). On the other hand, vancomycin and linezolid showed promising results where 100 % of the isolates were susceptible. Conclusion: We spotlight that NDC spp. is not viewed as a contaminant organism anymore, particularly in immunosuppressed patients. Timely identification, proper clinical correlation, and appropriate therapeutic intervention can lead to favorable outcomes.https://mid.journals.ekb.eg/article_339747_497d7d39c2eae5f4635cb8ef36e9aca9.pdfnon-diphtheriae corynebacteriumcorynebacterium striatummaldi-tofidentificationantibiotic resistance |
| spellingShingle | Nada Elwalaly Manal Abd El Alim. sally saber Sherin El Masry Noha Fahim Identification and antibiogram of clinically relevant non-diphtheriae Corynebacterium species Microbes and Infectious Diseases non-diphtheriae corynebacterium corynebacterium striatum maldi-tof identification antibiotic resistance |
| title | Identification and antibiogram of clinically relevant non-diphtheriae Corynebacterium species |
| title_full | Identification and antibiogram of clinically relevant non-diphtheriae Corynebacterium species |
| title_fullStr | Identification and antibiogram of clinically relevant non-diphtheriae Corynebacterium species |
| title_full_unstemmed | Identification and antibiogram of clinically relevant non-diphtheriae Corynebacterium species |
| title_short | Identification and antibiogram of clinically relevant non-diphtheriae Corynebacterium species |
| title_sort | identification and antibiogram of clinically relevant non diphtheriae corynebacterium species |
| topic | non-diphtheriae corynebacterium corynebacterium striatum maldi-tof identification antibiotic resistance |
| url | https://mid.journals.ekb.eg/article_339747_497d7d39c2eae5f4635cb8ef36e9aca9.pdf |
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