A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression

A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression

Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) plays a crucial role in relative gene expression analysis, and accurate normalization relies on suitable reference genes (RGs). In this study, a pipeline for identifying candidate RGs from publicly available stem-relate...

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Bibliographic Details
Main Authors: Qi Xie, Umair Ahmed, Cheng Qi, Kebing Du, Jie Luo, Pengcheng Wang, Bo Zheng, Xueping Shi
Format: Article
Language:English
Published: Maximum Academic Press 2024-01-01
Series:Forestry Research
Subjects:
reference genes
populus
gene expression
transcriptome
rt-qpcr
abiotic stress
stem development
Online Access:https://www.maxapress.com/article/doi/10.48130/forres-0024-0017
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