Alternative source of chitosan for the direct laser synthesis of Ag@chitosan composites with antibacterial and photocatalytic properties

In this study, we used chitosan obtained from the pupal exuviae of the insect Hermetia illucens to produced silver nanoparticles (Ag@CSE) by nanosecond laser ablation in liquid technique. The physic-chemical and functional properties of the obtained nanocomposite were compared to Ag@CS prepared by u...

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Main Authors: M. Marsico, A. Guarnieri, M. Triunfo, M. Curcio, A. Galasso, C. Scieuzo, R. Salvia, P. Falabella, R. Teghil, A. De Bonis
Format: Article
Language:English
Published: Elsevier 2025-10-01
Series:Next Materials
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Online Access:http://www.sciencedirect.com/science/article/pii/S2949822825004708
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author M. Marsico
A. Guarnieri
M. Triunfo
M. Curcio
A. Galasso
C. Scieuzo
R. Salvia
P. Falabella
R. Teghil
A. De Bonis
author_facet M. Marsico
A. Guarnieri
M. Triunfo
M. Curcio
A. Galasso
C. Scieuzo
R. Salvia
P. Falabella
R. Teghil
A. De Bonis
author_sort M. Marsico
collection DOAJ
description In this study, we used chitosan obtained from the pupal exuviae of the insect Hermetia illucens to produced silver nanoparticles (Ag@CSE) by nanosecond laser ablation in liquid technique. The physic-chemical and functional properties of the obtained nanocomposite were compared to Ag@CS prepared by using a solution of chitosan from crustaceans as liquid, in the same experimental conditions. The composites were studied by transmission electron microscopy (TEM), Uv-vis and FTIR and X-ray photoelectron spectroscopy (XPS) and X-ray diffractometry (XRD). The composites exhibit optical absorption at around 400 nm due to the presence of silver nanoparticles with a diameter of 11 and 14 nm for Ag@CS and Ag@CSE, respectively. We evaluated the antimicrobial capacity of these nanocomposites against two bacterial strains, Escherichia coli and Micrococcus flavus, by agar diffusion test and microdilution assay. Both composites showed a zone of inhibition of approximately 9 mm against both bacterial strains. Ag@CSE showed marked antimicrobial activity against E. coli up to 0.006 g/L, at which value Ag NPs alone lost their activity. The ability to photocatalyse the methylene blue degradation reaction of Ag@CS and Ag@CSE was tested under simulated solar irradiation conditions. The obtained results suggest that chitosan from HE is a valuable alternative source to chitosan from crustaceans and that the obtained composites can be considered for biomedical and photodegradation applications.
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spelling doaj-art-1fb8cecf98a0490f8f9cec621d2d7a772025-08-20T03:51:08ZengElsevierNext Materials2949-82282025-10-01910095210.1016/j.nxmate.2025.100952Alternative source of chitosan for the direct laser synthesis of Ag@chitosan composites with antibacterial and photocatalytic propertiesM. Marsico0A. Guarnieri1M. Triunfo2M. Curcio3A. Galasso4C. Scieuzo5R. Salvia6P. Falabella7R. Teghil8A. De Bonis9Dipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, ItalyDipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, ItalyDipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, ItalyDipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, ItalyDipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, ItalyDipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, Italy; Spinoff XFlies s.r.l, University of Basilicata, Potenza, ItalyDipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, Italy; Spinoff XFlies s.r.l, University of Basilicata, Potenza, ItalyDipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, Italy; Spinoff XFlies s.r.l, University of Basilicata, Potenza, ItalyDipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, ItalyDipartimento di Scienze di Base e Applicate - DISBA, Università della Basilicata, viale dell’Ateneo Lucano, 10, Potenza 85100, Italy; Corresponding author.In this study, we used chitosan obtained from the pupal exuviae of the insect Hermetia illucens to produced silver nanoparticles (Ag@CSE) by nanosecond laser ablation in liquid technique. The physic-chemical and functional properties of the obtained nanocomposite were compared to Ag@CS prepared by using a solution of chitosan from crustaceans as liquid, in the same experimental conditions. The composites were studied by transmission electron microscopy (TEM), Uv-vis and FTIR and X-ray photoelectron spectroscopy (XPS) and X-ray diffractometry (XRD). The composites exhibit optical absorption at around 400 nm due to the presence of silver nanoparticles with a diameter of 11 and 14 nm for Ag@CS and Ag@CSE, respectively. We evaluated the antimicrobial capacity of these nanocomposites against two bacterial strains, Escherichia coli and Micrococcus flavus, by agar diffusion test and microdilution assay. Both composites showed a zone of inhibition of approximately 9 mm against both bacterial strains. Ag@CSE showed marked antimicrobial activity against E. coli up to 0.006 g/L, at which value Ag NPs alone lost their activity. The ability to photocatalyse the methylene blue degradation reaction of Ag@CS and Ag@CSE was tested under simulated solar irradiation conditions. The obtained results suggest that chitosan from HE is a valuable alternative source to chitosan from crustaceans and that the obtained composites can be considered for biomedical and photodegradation applications.http://www.sciencedirect.com/science/article/pii/S2949822825004708ChitosanSilver nanoparticlesLaser ablation in liquidHermetia illucensAntibacterial activityPhotodegradation
spellingShingle M. Marsico
A. Guarnieri
M. Triunfo
M. Curcio
A. Galasso
C. Scieuzo
R. Salvia
P. Falabella
R. Teghil
A. De Bonis
Alternative source of chitosan for the direct laser synthesis of Ag@chitosan composites with antibacterial and photocatalytic properties
Next Materials
Chitosan
Silver nanoparticles
Laser ablation in liquid
Hermetia illucens
Antibacterial activity
Photodegradation
title Alternative source of chitosan for the direct laser synthesis of Ag@chitosan composites with antibacterial and photocatalytic properties
title_full Alternative source of chitosan for the direct laser synthesis of Ag@chitosan composites with antibacterial and photocatalytic properties
title_fullStr Alternative source of chitosan for the direct laser synthesis of Ag@chitosan composites with antibacterial and photocatalytic properties
title_full_unstemmed Alternative source of chitosan for the direct laser synthesis of Ag@chitosan composites with antibacterial and photocatalytic properties
title_short Alternative source of chitosan for the direct laser synthesis of Ag@chitosan composites with antibacterial and photocatalytic properties
title_sort alternative source of chitosan for the direct laser synthesis of ag chitosan composites with antibacterial and photocatalytic properties
topic Chitosan
Silver nanoparticles
Laser ablation in liquid
Hermetia illucens
Antibacterial activity
Photodegradation
url http://www.sciencedirect.com/science/article/pii/S2949822825004708
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