Phytochemical Profiling and Biological Evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i>: A Chromatographic and Mass Spectrometry Approach to Uncovering Bioactive Metabolites for Dermatological and Metabolic Disorder Management
<b>Background/Objectives</b>: This study provides the first comprehensive phytochemical composition and biological evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i> (Batt.) Greuter & Burdet, a plant endemic to Algeria with unexplored pharmacolog...
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2025-04-01
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| author | Amina Bouzana Zohra Chekroud Imène Becheker Fatima Kamah Nora Sakhraoui Chawki Bensouici Fehmi Boufahja Sulaiman A. Alsalamah Mohammed I. Alghonaim Stefania Garzoli Hamdi Bendif |
| author_facet | Amina Bouzana Zohra Chekroud Imène Becheker Fatima Kamah Nora Sakhraoui Chawki Bensouici Fehmi Boufahja Sulaiman A. Alsalamah Mohammed I. Alghonaim Stefania Garzoli Hamdi Bendif |
| author_sort | Amina Bouzana |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: This study provides the first comprehensive phytochemical composition and biological evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i> (Batt.) Greuter & Burdet, a plant endemic to Algeria with unexplored pharmacological potential. The objective is to identify novel bioactive metabolites in the plant’s extracts and assess their potential applications for skincare and metabolic disorder management, addressing gaps in the current understanding of its medicinal value. <b>Methods</b>: Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) profiling was used to analyze the hydromethanolic (HMeOH) leaf extract and identify bioactive compounds. The biological activities of HMeOH, ethyl acetate (EtOAc), and butanolic (n-BuOH) extracts were tested for cytotoxicity using the brine shrimp lethality test, photoprotective potential by calculating the sun protection factor (SPF), and enzymatic inhibitory activities against alpha-amylase, urease, and tyrosinase. <b>Results</b>: The LC-ESI-MS/MS profiling of the MeOH extract identified 22 bioactive compounds, including phenolic acids and flavonoids, some of which have not been previously reported in this species. Cytotoxicity tests showed that all extracts were non-toxic (half-lethal concentration (LC<sub>50</sub>) > 100 micrograms per milliliter). The SPF values indicated significant photoprotective potential, with EtOAc (SPF = 45.19 ± 0.73) and n-BuOH (SPF = 43.81 ± 0.59) extracts showing high sun protection activity. The n-BuOH extract exhibited strong alpha-amylase inhibitory activity (half-maximal inhibitory concentration (IC<sub>50</sub>) = 307.08 micrograms per milliliter), surpassing the standard acarbose (IC<sub>50</sub> = 3650.93 micrograms per milliliter), suggesting potential applications in diabetes management. <b>Conclusions</b>: <i>Dianthus sylvestris</i> subsp. <i>aristidis</i> demonstrates significant pharmacological potential as a source of bioactive secondary metabolites for skincare and metabolic disorder management. These findings provide new insights into the plant’s therapeutic potential and set a foundation for future pharmacological and clinical investigations. |
| format | Article |
| id | doaj-art-1fb3de4b48ee48b58f8adea64b791019 |
| institution | OA Journals |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-1fb3de4b48ee48b58f8adea64b7910192025-08-20T02:28:25ZengMDPI AGPharmaceuticals1424-82472025-04-0118457810.3390/ph18040578Phytochemical Profiling and Biological Evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i>: A Chromatographic and Mass Spectrometry Approach to Uncovering Bioactive Metabolites for Dermatological and Metabolic Disorder ManagementAmina Bouzana0Zohra Chekroud1Imène Becheker2Fatima Kamah3Nora Sakhraoui4Chawki Bensouici5Fehmi Boufahja6Sulaiman A. Alsalamah7Mohammed I. Alghonaim8Stefania Garzoli9Hamdi Bendif10Laboratory of Interactions, Biodiversity, Ecosystems and Biotechnology, Department of Nature and Life Sciences, Faculty of Sciences, University 20 August 1955 Skikda, Skikda 21000, AlgeriaLaboratory of Interactions, Biodiversity, Ecosystems and Biotechnology, Department of Nature and Life Sciences, Faculty of Sciences, University 20 August 1955 Skikda, Skikda 21000, AlgeriaLaboratory of Interactions, Biodiversity, Ecosystems and Biotechnology, Department of Nature and Life Sciences, Faculty of Sciences, University 20 August 1955 Skikda, Skikda 21000, AlgeriaLaboratory of Interactions, Biodiversity, Ecosystems and Biotechnology, Department of Nature and Life Sciences, Faculty of Sciences, University 20 August 1955 Skikda, Skikda 21000, AlgeriaLaboratory of Interactions, Biodiversity, Ecosystems and Biotechnology, Department of Ecology and Environment, Faculty of Sciences, University 20 August 1955 Skikda, Skikda 21000, AlgeriaBiotechnology Research Center, Ali Mendjli New City UV 03, BP E73, Constantine 25016, AlgeriaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi ArabiaDepartment of Chemistry and Technologies of Drug, Sapienza University, P. le Aldo Moro, 5, 00185 Rome, ItalyBiology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia<b>Background/Objectives</b>: This study provides the first comprehensive phytochemical composition and biological evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i> (Batt.) Greuter & Burdet, a plant endemic to Algeria with unexplored pharmacological potential. The objective is to identify novel bioactive metabolites in the plant’s extracts and assess their potential applications for skincare and metabolic disorder management, addressing gaps in the current understanding of its medicinal value. <b>Methods</b>: Liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) profiling was used to analyze the hydromethanolic (HMeOH) leaf extract and identify bioactive compounds. The biological activities of HMeOH, ethyl acetate (EtOAc), and butanolic (n-BuOH) extracts were tested for cytotoxicity using the brine shrimp lethality test, photoprotective potential by calculating the sun protection factor (SPF), and enzymatic inhibitory activities against alpha-amylase, urease, and tyrosinase. <b>Results</b>: The LC-ESI-MS/MS profiling of the MeOH extract identified 22 bioactive compounds, including phenolic acids and flavonoids, some of which have not been previously reported in this species. Cytotoxicity tests showed that all extracts were non-toxic (half-lethal concentration (LC<sub>50</sub>) > 100 micrograms per milliliter). The SPF values indicated significant photoprotective potential, with EtOAc (SPF = 45.19 ± 0.73) and n-BuOH (SPF = 43.81 ± 0.59) extracts showing high sun protection activity. The n-BuOH extract exhibited strong alpha-amylase inhibitory activity (half-maximal inhibitory concentration (IC<sub>50</sub>) = 307.08 micrograms per milliliter), surpassing the standard acarbose (IC<sub>50</sub> = 3650.93 micrograms per milliliter), suggesting potential applications in diabetes management. <b>Conclusions</b>: <i>Dianthus sylvestris</i> subsp. <i>aristidis</i> demonstrates significant pharmacological potential as a source of bioactive secondary metabolites for skincare and metabolic disorder management. These findings provide new insights into the plant’s therapeutic potential and set a foundation for future pharmacological and clinical investigations.https://www.mdpi.com/1424-8247/18/4/578<i>Dianthus sylvestris</i> subsp. <i>aristidis</i>phytochemical profilingLC-ESI-MS/MSenzymatic inhibitory activitiesphotoprotective potentialsecondary metabolites |
| spellingShingle | Amina Bouzana Zohra Chekroud Imène Becheker Fatima Kamah Nora Sakhraoui Chawki Bensouici Fehmi Boufahja Sulaiman A. Alsalamah Mohammed I. Alghonaim Stefania Garzoli Hamdi Bendif Phytochemical Profiling and Biological Evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i>: A Chromatographic and Mass Spectrometry Approach to Uncovering Bioactive Metabolites for Dermatological and Metabolic Disorder Management Pharmaceuticals <i>Dianthus sylvestris</i> subsp. <i>aristidis</i> phytochemical profiling LC-ESI-MS/MS enzymatic inhibitory activities photoprotective potential secondary metabolites |
| title | Phytochemical Profiling and Biological Evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i>: A Chromatographic and Mass Spectrometry Approach to Uncovering Bioactive Metabolites for Dermatological and Metabolic Disorder Management |
| title_full | Phytochemical Profiling and Biological Evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i>: A Chromatographic and Mass Spectrometry Approach to Uncovering Bioactive Metabolites for Dermatological and Metabolic Disorder Management |
| title_fullStr | Phytochemical Profiling and Biological Evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i>: A Chromatographic and Mass Spectrometry Approach to Uncovering Bioactive Metabolites for Dermatological and Metabolic Disorder Management |
| title_full_unstemmed | Phytochemical Profiling and Biological Evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i>: A Chromatographic and Mass Spectrometry Approach to Uncovering Bioactive Metabolites for Dermatological and Metabolic Disorder Management |
| title_short | Phytochemical Profiling and Biological Evaluation of <i>Dianthus sylvestris</i> subsp. <i>aristidis</i>: A Chromatographic and Mass Spectrometry Approach to Uncovering Bioactive Metabolites for Dermatological and Metabolic Disorder Management |
| title_sort | phytochemical profiling and biological evaluation of i dianthus sylvestris i subsp i aristidis i a chromatographic and mass spectrometry approach to uncovering bioactive metabolites for dermatological and metabolic disorder management |
| topic | <i>Dianthus sylvestris</i> subsp. <i>aristidis</i> phytochemical profiling LC-ESI-MS/MS enzymatic inhibitory activities photoprotective potential secondary metabolites |
| url | https://www.mdpi.com/1424-8247/18/4/578 |
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