Extraction, Characterization, and Impact of Polysaccharides From Zhonghuang No. 1 Etiolated‐Green Tea on Human Fecal Microbiota
ABSTRACT Tea polysaccharides have attracted widespread attention in regulating gut microbiota. A highly efficient enzymolysis and ultrasonic‐assisted extraction method was developed and optimized for the extraction of polysaccharides from Zhonghuang No. 1 etiolated‐green tea (GTP) with a‐single fact...
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| Format: | Article |
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Wiley
2025-06-01
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| Series: | Food Bioengineering |
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| Online Access: | https://doi.org/10.1002/fbe2.70016 |
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| author | Baoming Tian Xue Zhou Shuo Pan Wei Liu |
| author_facet | Baoming Tian Xue Zhou Shuo Pan Wei Liu |
| author_sort | Baoming Tian |
| collection | DOAJ |
| description | ABSTRACT Tea polysaccharides have attracted widespread attention in regulating gut microbiota. A highly efficient enzymolysis and ultrasonic‐assisted extraction method was developed and optimized for the extraction of polysaccharides from Zhonghuang No. 1 etiolated‐green tea (GTP) with a‐single factor and response surface methodology, i.e, with a pH of 5.5, an enzyme concentration of 7%, an ultrasonic time of 2.5 h, a solid‐liquid ratio of 1:20 g/mL, and an ultrasonic temperature of 80°C. GTP exhibited a triple helix structure and demonstrated the ability to bind with Congo red. Its surface was observed to be spherical with slender chain fragments. GTP remained intact throughout the simulated in vitro digestive process, which encompassed the oral, gastric, and small intestinal phases. The relative abundance of Bacteroidota was significantly increased, while the Firmicutes/Bacteroidota ratio was reduced following the administration of GTP. The contents of Faecalibacterium and Bacteroides were increased, while the contents of Escherichia_Shigella and Dorea were reduced after GTP treatment. Furthermore, GTP were degraded and utilized by gut microbiota to produce SCFAs and reducing pH value. GTP regulated the composition of the gut microbiota and has the potential to act as a prebiotic, with the possibility of its use as a functional food. |
| format | Article |
| id | doaj-art-1fb24c8d2cfc496c93ce66097985f264 |
| institution | Kabale University |
| issn | 2770-2081 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
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| series | Food Bioengineering |
| spelling | doaj-art-1fb24c8d2cfc496c93ce66097985f2642025-08-20T03:58:45ZengWileyFood Bioengineering2770-20812025-06-014216618110.1002/fbe2.70016Extraction, Characterization, and Impact of Polysaccharides From Zhonghuang No. 1 Etiolated‐Green Tea on Human Fecal MicrobiotaBaoming Tian0Xue Zhou1Shuo Pan2Wei Liu3College of Food Science and Technology Zhejiang University of Technology Huzhou ChinaCollege of Food Science and Technology Zhejiang University of Technology Huzhou ChinaCollege of Food Science and Technology Zhejiang University of Technology Huzhou ChinaInstitute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Sciences Hangzhou ChinaABSTRACT Tea polysaccharides have attracted widespread attention in regulating gut microbiota. A highly efficient enzymolysis and ultrasonic‐assisted extraction method was developed and optimized for the extraction of polysaccharides from Zhonghuang No. 1 etiolated‐green tea (GTP) with a‐single factor and response surface methodology, i.e, with a pH of 5.5, an enzyme concentration of 7%, an ultrasonic time of 2.5 h, a solid‐liquid ratio of 1:20 g/mL, and an ultrasonic temperature of 80°C. GTP exhibited a triple helix structure and demonstrated the ability to bind with Congo red. Its surface was observed to be spherical with slender chain fragments. GTP remained intact throughout the simulated in vitro digestive process, which encompassed the oral, gastric, and small intestinal phases. The relative abundance of Bacteroidota was significantly increased, while the Firmicutes/Bacteroidota ratio was reduced following the administration of GTP. The contents of Faecalibacterium and Bacteroides were increased, while the contents of Escherichia_Shigella and Dorea were reduced after GTP treatment. Furthermore, GTP were degraded and utilized by gut microbiota to produce SCFAs and reducing pH value. GTP regulated the composition of the gut microbiota and has the potential to act as a prebiotic, with the possibility of its use as a functional food.https://doi.org/10.1002/fbe2.70016gut microbiotain vitro fermentationPolysaccharides from etiolated‐green teashort‐chain fatty acidssimulated digestion |
| spellingShingle | Baoming Tian Xue Zhou Shuo Pan Wei Liu Extraction, Characterization, and Impact of Polysaccharides From Zhonghuang No. 1 Etiolated‐Green Tea on Human Fecal Microbiota Food Bioengineering gut microbiota in vitro fermentation Polysaccharides from etiolated‐green tea short‐chain fatty acids simulated digestion |
| title | Extraction, Characterization, and Impact of Polysaccharides From Zhonghuang No. 1 Etiolated‐Green Tea on Human Fecal Microbiota |
| title_full | Extraction, Characterization, and Impact of Polysaccharides From Zhonghuang No. 1 Etiolated‐Green Tea on Human Fecal Microbiota |
| title_fullStr | Extraction, Characterization, and Impact of Polysaccharides From Zhonghuang No. 1 Etiolated‐Green Tea on Human Fecal Microbiota |
| title_full_unstemmed | Extraction, Characterization, and Impact of Polysaccharides From Zhonghuang No. 1 Etiolated‐Green Tea on Human Fecal Microbiota |
| title_short | Extraction, Characterization, and Impact of Polysaccharides From Zhonghuang No. 1 Etiolated‐Green Tea on Human Fecal Microbiota |
| title_sort | extraction characterization and impact of polysaccharides from zhonghuang no 1 etiolated green tea on human fecal microbiota |
| topic | gut microbiota in vitro fermentation Polysaccharides from etiolated‐green tea short‐chain fatty acids simulated digestion |
| url | https://doi.org/10.1002/fbe2.70016 |
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