Apolipoprotein E Antibodies Affect the Retention of Passive Avoidance Memory in the Chick

Isoforms of apolipoprotein E (ApoE) have been implicated as risk factors in Alzheimer’s disease. We have, therefore, examined the possible role of ApoE in memory formation, using a one-trial passive avoidance task in day-old chicks. Birds were trained on the task and then at various times pre or pos...

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Main Authors: Chris Lancashire, Radmila Mileusnic, Steven P.R. Rose
Format: Article
Language:English
Published: Wiley 1998-01-01
Series:Neural Plasticity
Online Access:http://dx.doi.org/10.1155/NP.1998.29
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author Chris Lancashire
Radmila Mileusnic
Steven P.R. Rose
author_facet Chris Lancashire
Radmila Mileusnic
Steven P.R. Rose
author_sort Chris Lancashire
collection DOAJ
description Isoforms of apolipoprotein E (ApoE) have been implicated as risk factors in Alzheimer’s disease. We have, therefore, examined the possible role of ApoE in memory formation, using a one-trial passive avoidance task in day-old chicks. Birds were trained on the task and then at various times pre or post-training were injected intracerebrally with anti-ApoE. Immunofluorescence staining demonstrated the presence of the antibody bound to the neuropil, close to the injection site and adjacent to the ventricle, with a residence time in the brain of up to 30 min. Chicks that were injected 30 min pre-training or just post-training with 5μg/ hemisphere of the antibody learned the task, but were amnesic when tested at 30 min or at subsequent times up to 24 hr Post-training. When tested at 24 hr, birds injected 5.5 hr post-training showed unimpaired retention. Birds injected with 5μg/hemisphere of anti-ApoA-I (which has a brain distribution similar to that of anti-ApoE) at 30 min pretraining showed no amnesia, indicating the specificity of the effect to the ApoE. Possible mechanisms for this effect are discussed.
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spelling doaj-art-1f9aad45a34f4569bce6fe2ed0c9e6772025-08-20T03:54:34ZengWileyNeural Plasticity2090-59041687-54431998-01-0163294010.1155/NP.1998.29Apolipoprotein E Antibodies Affect the Retention of Passive Avoidance Memory in the ChickChris Lancashire0Radmila Mileusnic1Steven P.R. Rose2Brain and Behaviour Research Group, The Open University, Milton Keynes MK7 6AA, UKBrain and Behaviour Research Group, The Open University, Milton Keynes MK7 6AA, UKBrain and Behaviour Research Group, The Open University, Milton Keynes MK7 6AA, UKIsoforms of apolipoprotein E (ApoE) have been implicated as risk factors in Alzheimer’s disease. We have, therefore, examined the possible role of ApoE in memory formation, using a one-trial passive avoidance task in day-old chicks. Birds were trained on the task and then at various times pre or post-training were injected intracerebrally with anti-ApoE. Immunofluorescence staining demonstrated the presence of the antibody bound to the neuropil, close to the injection site and adjacent to the ventricle, with a residence time in the brain of up to 30 min. Chicks that were injected 30 min pre-training or just post-training with 5μg/ hemisphere of the antibody learned the task, but were amnesic when tested at 30 min or at subsequent times up to 24 hr Post-training. When tested at 24 hr, birds injected 5.5 hr post-training showed unimpaired retention. Birds injected with 5μg/hemisphere of anti-ApoA-I (which has a brain distribution similar to that of anti-ApoE) at 30 min pretraining showed no amnesia, indicating the specificity of the effect to the ApoE. Possible mechanisms for this effect are discussed.http://dx.doi.org/10.1155/NP.1998.29
spellingShingle Chris Lancashire
Radmila Mileusnic
Steven P.R. Rose
Apolipoprotein E Antibodies Affect the Retention of Passive Avoidance Memory in the Chick
Neural Plasticity
title Apolipoprotein E Antibodies Affect the Retention of Passive Avoidance Memory in the Chick
title_full Apolipoprotein E Antibodies Affect the Retention of Passive Avoidance Memory in the Chick
title_fullStr Apolipoprotein E Antibodies Affect the Retention of Passive Avoidance Memory in the Chick
title_full_unstemmed Apolipoprotein E Antibodies Affect the Retention of Passive Avoidance Memory in the Chick
title_short Apolipoprotein E Antibodies Affect the Retention of Passive Avoidance Memory in the Chick
title_sort apolipoprotein e antibodies affect the retention of passive avoidance memory in the chick
url http://dx.doi.org/10.1155/NP.1998.29
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