Dimethyl Isosorbide: An Innovative Bio-Renewable Solvent for Sustainable Chromatographic Applications

The increasing environmental concerns and regulatory restrictions on toxic conventional solvents have driven the search for sustainable alternatives. Dimethyl isosorbide (DMI), a bio-renewable solvent, has shown potential as a replacement for short-chain glycol ethers, although its use as solvent in...

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Main Authors: Aleksandra Damjanoska, Kristina Mitreska, Matilda Petrova, Jelena Acevska, Katerina Brezovska, Natalija Nakov
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/13/2713
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author Aleksandra Damjanoska
Kristina Mitreska
Matilda Petrova
Jelena Acevska
Katerina Brezovska
Natalija Nakov
author_facet Aleksandra Damjanoska
Kristina Mitreska
Matilda Petrova
Jelena Acevska
Katerina Brezovska
Natalija Nakov
author_sort Aleksandra Damjanoska
collection DOAJ
description The increasing environmental concerns and regulatory restrictions on toxic conventional solvents have driven the search for sustainable alternatives. Dimethyl isosorbide (DMI), a bio-renewable solvent, has shown potential as a replacement for short-chain glycol ethers, although its use as solvent in liquid chromatography (LC) is underexplored. This study presents a physicochemical characterization of DMI with a particular focus on its application as an innovative solvent in LC analyses. The partition coefficient (<i>log P</i> = −0.44) was determined using the OECD 107 method, and viscosity measurements for DMI and its mixtures with water and ethanol were conducted at 25 °C, 40 °C, and 60 °C. Viscosity ranged from 1.28 mPa·s at 60 °C to 2.62 mPa·s at 40 °C. The Central Composite Face 2<sup>3</sup> experimental design for studying the chromatographic behavior of DMI confirmed that 50% (<i>v</i>/<i>v</i>) DMI can be effectively utilized in the mobile phases, at a column temperature of 40 °C, with backpressures ranging from 160 to 300 bar and a UV cut-off at 240 nm. Its effectiveness as an eluent in LC was demonstrated for the quantification of methylparaben and propylparaben in pharmaceutical formulations. This study highlights DMI’s promise as a sustainable bio-renewable alternative to conventional organic solvents used as eluents in LC, supporting eco-friendly practices in pharmaceutical analysis.
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spelling doaj-art-1f85519ab2214e2aac5726dc87fc08122025-08-20T02:36:27ZengMDPI AGMolecules1420-30492025-06-013013271310.3390/molecules30132713Dimethyl Isosorbide: An Innovative Bio-Renewable Solvent for Sustainable Chromatographic ApplicationsAleksandra Damjanoska0Kristina Mitreska1Matilda Petrova2Jelena Acevska3Katerina Brezovska4Natalija Nakov5Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North MacedoniaInstitute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North MacedoniaManufacturing Technical Operations, ALKALOID AD, Blvd. Aleksandar Makedonski 12, 1000 Skopje, North MacedoniaInstitute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North MacedoniaInstitute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North MacedoniaInstitute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Mother Tereza 47, 1000 Skopje, North MacedoniaThe increasing environmental concerns and regulatory restrictions on toxic conventional solvents have driven the search for sustainable alternatives. Dimethyl isosorbide (DMI), a bio-renewable solvent, has shown potential as a replacement for short-chain glycol ethers, although its use as solvent in liquid chromatography (LC) is underexplored. This study presents a physicochemical characterization of DMI with a particular focus on its application as an innovative solvent in LC analyses. The partition coefficient (<i>log P</i> = −0.44) was determined using the OECD 107 method, and viscosity measurements for DMI and its mixtures with water and ethanol were conducted at 25 °C, 40 °C, and 60 °C. Viscosity ranged from 1.28 mPa·s at 60 °C to 2.62 mPa·s at 40 °C. The Central Composite Face 2<sup>3</sup> experimental design for studying the chromatographic behavior of DMI confirmed that 50% (<i>v</i>/<i>v</i>) DMI can be effectively utilized in the mobile phases, at a column temperature of 40 °C, with backpressures ranging from 160 to 300 bar and a UV cut-off at 240 nm. Its effectiveness as an eluent in LC was demonstrated for the quantification of methylparaben and propylparaben in pharmaceutical formulations. This study highlights DMI’s promise as a sustainable bio-renewable alternative to conventional organic solvents used as eluents in LC, supporting eco-friendly practices in pharmaceutical analysis.https://www.mdpi.com/1420-3049/30/13/2713dimethyl isosorbidebio-renewable solventliquid chromatographygreen chemistrysustainabilitypartition coefficient
spellingShingle Aleksandra Damjanoska
Kristina Mitreska
Matilda Petrova
Jelena Acevska
Katerina Brezovska
Natalija Nakov
Dimethyl Isosorbide: An Innovative Bio-Renewable Solvent for Sustainable Chromatographic Applications
Molecules
dimethyl isosorbide
bio-renewable solvent
liquid chromatography
green chemistry
sustainability
partition coefficient
title Dimethyl Isosorbide: An Innovative Bio-Renewable Solvent for Sustainable Chromatographic Applications
title_full Dimethyl Isosorbide: An Innovative Bio-Renewable Solvent for Sustainable Chromatographic Applications
title_fullStr Dimethyl Isosorbide: An Innovative Bio-Renewable Solvent for Sustainable Chromatographic Applications
title_full_unstemmed Dimethyl Isosorbide: An Innovative Bio-Renewable Solvent for Sustainable Chromatographic Applications
title_short Dimethyl Isosorbide: An Innovative Bio-Renewable Solvent for Sustainable Chromatographic Applications
title_sort dimethyl isosorbide an innovative bio renewable solvent for sustainable chromatographic applications
topic dimethyl isosorbide
bio-renewable solvent
liquid chromatography
green chemistry
sustainability
partition coefficient
url https://www.mdpi.com/1420-3049/30/13/2713
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AT matildapetrova dimethylisosorbideaninnovativebiorenewablesolventforsustainablechromatographicapplications
AT jelenaacevska dimethylisosorbideaninnovativebiorenewablesolventforsustainablechromatographicapplications
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