Icarisid Ⅱ modulates mitochondrial dynamics for anti-HBV activity
IntroductionTo investigate the potential anti-hepatitis B virus (HBV) activity of Icariside Ⅱ (ICS Ⅱ), and elucidate its underlying mitochondrial dynamics mechanisms.MethodsThe study employed in vivo and in vitro assays to evaluate anti-HBV effects of ICS Ⅱ. An HBV replicating mouse model was establ...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1544714/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850049335946903552 |
|---|---|
| author | Zhengyun Liu Haiyan Yang Juan Wen Dongyan Xiao Dongyan Xiao Wan Yu Guo Luo Qihai Gong Huan Wang |
| author_facet | Zhengyun Liu Haiyan Yang Juan Wen Dongyan Xiao Dongyan Xiao Wan Yu Guo Luo Qihai Gong Huan Wang |
| author_sort | Zhengyun Liu |
| collection | DOAJ |
| description | IntroductionTo investigate the potential anti-hepatitis B virus (HBV) activity of Icariside Ⅱ (ICS Ⅱ), and elucidate its underlying mitochondrial dynamics mechanisms.MethodsThe study employed in vivo and in vitro assays to evaluate anti-HBV effects of ICS Ⅱ. An HBV replicating mouse model was established through hydrodynamic injection of pAAV/HBV1.2, the impact of ICS Ⅱ on HBV replication and liver toxicity was assessed. In vitro cell-based assays used HBV-positive HepG2.2.15 cells. Cytotoxicity was determined with CCK-8 assay, while ELISA and qPCR were employed to measure HBsAg, HBeAg, and HBV DNA levels. The livers of ICS II-treated HBV-infected mice were taken for transcriptome sequencing to screen for different genes, and the results were verified by Western Blot. Mitochondrial morphology and dynamics were visualized using confocal imaging and transmission electron microscopy. Key protein expressions related to mitochondrial fission and fusion were analyzed via WB. Intracellular ROS generation was assessed using fluorescence staining.ResultsThe study found that ICS Ⅱ exhibited significant anti-HBV effects both in vivo and in vitro. The results of RNA-Seq indicated that ICS Ⅱ modulated the mRNA levels of Fisl, a protein associated with mitochondrial dynamics, during the anti-HBV response. It induced mitochondrial fragmentation and enhanced mitochondrial motility in HBV-positive cells. Notably, key proteins associated with mitochondrial fission and fusion demonstrated alterations favoring fission. Furthermore, ICS Ⅱ effectively reduced ROS production in HBV-positive cells.ConclusionICS Ⅱ exhibits significant anti-HBV potential through its regulation of mitochondrial dynamics and ROS production. |
| format | Article |
| id | doaj-art-1f7fc0c794a147dfb7830c92a06b37bc |
| institution | DOAJ |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-1f7fc0c794a147dfb7830c92a06b37bc2025-08-20T02:53:44ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-04-011610.3389/fphar.2025.15447141544714Icarisid Ⅱ modulates mitochondrial dynamics for anti-HBV activityZhengyun Liu0Haiyan Yang1Juan Wen2Dongyan Xiao3Dongyan Xiao4Wan Yu5Guo Luo6Qihai Gong7Huan Wang8Key Laboratory of Infectious Disease and Biosafety, Provincial Department of Education, Zunyi Medical University, Zunyi, Guizhou, ChinaKey Laboratory of Infectious Disease and Biosafety, Provincial Department of Education, Zunyi Medical University, Zunyi, Guizhou, ChinaKey Laboratory of Infectious Disease and Biosafety, Provincial Department of Education, Zunyi Medical University, Zunyi, Guizhou, ChinaKey Laboratory of Infectious Disease and Biosafety, Provincial Department of Education, Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Basic Teaching, Zunyi Medical and Pharmaceutical College, Zunyi, Guizhou, ChinaKey Laboratory of Infectious Disease and Biosafety, Provincial Department of Education, Zunyi Medical University, Zunyi, Guizhou, ChinaKey Laboratory of Infectious Disease and Biosafety, Provincial Department of Education, Zunyi Medical University, Zunyi, Guizhou, ChinaKey Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou, ChinaKey Laboratory of Infectious Disease and Biosafety, Provincial Department of Education, Zunyi Medical University, Zunyi, Guizhou, ChinaIntroductionTo investigate the potential anti-hepatitis B virus (HBV) activity of Icariside Ⅱ (ICS Ⅱ), and elucidate its underlying mitochondrial dynamics mechanisms.MethodsThe study employed in vivo and in vitro assays to evaluate anti-HBV effects of ICS Ⅱ. An HBV replicating mouse model was established through hydrodynamic injection of pAAV/HBV1.2, the impact of ICS Ⅱ on HBV replication and liver toxicity was assessed. In vitro cell-based assays used HBV-positive HepG2.2.15 cells. Cytotoxicity was determined with CCK-8 assay, while ELISA and qPCR were employed to measure HBsAg, HBeAg, and HBV DNA levels. The livers of ICS II-treated HBV-infected mice were taken for transcriptome sequencing to screen for different genes, and the results were verified by Western Blot. Mitochondrial morphology and dynamics were visualized using confocal imaging and transmission electron microscopy. Key protein expressions related to mitochondrial fission and fusion were analyzed via WB. Intracellular ROS generation was assessed using fluorescence staining.ResultsThe study found that ICS Ⅱ exhibited significant anti-HBV effects both in vivo and in vitro. The results of RNA-Seq indicated that ICS Ⅱ modulated the mRNA levels of Fisl, a protein associated with mitochondrial dynamics, during the anti-HBV response. It induced mitochondrial fragmentation and enhanced mitochondrial motility in HBV-positive cells. Notably, key proteins associated with mitochondrial fission and fusion demonstrated alterations favoring fission. Furthermore, ICS Ⅱ effectively reduced ROS production in HBV-positive cells.ConclusionICS Ⅱ exhibits significant anti-HBV potential through its regulation of mitochondrial dynamics and ROS production.https://www.frontiersin.org/articles/10.3389/fphar.2025.1544714/fullhepatitis B virusHepG2.2.15 cellmitochondrial dynamicROSreactive oxygen speciesicariside II |
| spellingShingle | Zhengyun Liu Haiyan Yang Juan Wen Dongyan Xiao Dongyan Xiao Wan Yu Guo Luo Qihai Gong Huan Wang Icarisid Ⅱ modulates mitochondrial dynamics for anti-HBV activity Frontiers in Pharmacology hepatitis B virus HepG2.2.15 cell mitochondrial dynamic ROS reactive oxygen species icariside II |
| title | Icarisid Ⅱ modulates mitochondrial dynamics for anti-HBV activity |
| title_full | Icarisid Ⅱ modulates mitochondrial dynamics for anti-HBV activity |
| title_fullStr | Icarisid Ⅱ modulates mitochondrial dynamics for anti-HBV activity |
| title_full_unstemmed | Icarisid Ⅱ modulates mitochondrial dynamics for anti-HBV activity |
| title_short | Icarisid Ⅱ modulates mitochondrial dynamics for anti-HBV activity |
| title_sort | icarisid ii modulates mitochondrial dynamics for anti hbv activity |
| topic | hepatitis B virus HepG2.2.15 cell mitochondrial dynamic ROS reactive oxygen species icariside II |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1544714/full |
| work_keys_str_mv | AT zhengyunliu icarisidiimodulatesmitochondrialdynamicsforantihbvactivity AT haiyanyang icarisidiimodulatesmitochondrialdynamicsforantihbvactivity AT juanwen icarisidiimodulatesmitochondrialdynamicsforantihbvactivity AT dongyanxiao icarisidiimodulatesmitochondrialdynamicsforantihbvactivity AT dongyanxiao icarisidiimodulatesmitochondrialdynamicsforantihbvactivity AT wanyu icarisidiimodulatesmitochondrialdynamicsforantihbvactivity AT guoluo icarisidiimodulatesmitochondrialdynamicsforantihbvactivity AT qihaigong icarisidiimodulatesmitochondrialdynamicsforantihbvactivity AT huanwang icarisidiimodulatesmitochondrialdynamicsforantihbvactivity |