The effect of farnesol on the cAMP-PKA signaling pathway of Candida albicans biofilms and its correlation with drug resistance
Objective To explore the regulatory role of farnesol in Candida albicans (C. albicans) biofilm cAMP-PKA signaling pathway and its correlation with drug resistance. Methods Standard, fluconazole-resistant, wild and high RAS1 gene expression strains of C. albicans were cultured to different phases of...
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Editorial Office of Stomatology
2025-05-01
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| author | WANG Yang, CHEN Xueyi, YU Shenjun, CAO Xuejiao, WEI Xin, YANG Xuan |
| author_facet | WANG Yang, CHEN Xueyi, YU Shenjun, CAO Xuejiao, WEI Xin, YANG Xuan |
| author_sort | WANG Yang, CHEN Xueyi, YU Shenjun, CAO Xuejiao, WEI Xin, YANG Xuan |
| collection | DOAJ |
| description | Objective To explore the regulatory role of farnesol in Candida albicans (C. albicans) biofilm cAMP-PKA signaling pathway and its correlation with drug resistance. Methods Standard, fluconazole-resistant, wild and high RAS1 gene expression strains of C. albicans were cultured to different phases of the biofilm (6,12,24,36 h), and the sessile minimal inhibitory concentration 50%(SMIC50) of fluconazole were determined by XTT reduction after farnesol treatment. The regulatory effects of farnesol on the expression of genes related to the cAMP-PKA signaling pathway in standard and fluconazole-resistant strains of C. albicans, such as RAS1, CYR1, PDE2 were examined using qPCR; the effects of farnesol on the protein expression of the pathway were analyzed by Western blot. RAS1 gene expression of the wild and high RAS1 gene expression strains was measured by qPCR. Results ① Compared with the standard strain, resistant strains of C. albicans had higher levels of biofilm SMIC50 at 6, 12 and 24 h; there was no significant difference in RAS1 expression (P>0.05), while CYR1 expression increased significantly at 6 and 24 h in the biofilm (P<0.01), and PDE2 expression decreased at 6 h in the biofilm (P<0.01). ②After treatment with farnesol, the resistance of the biofilm of the standard strain and drug-resistant strain decreased. Compared with no treatment with farnesol, the expression of RAS1 in the biofilm of the standard strain and drug-resistant strain decreased at all time points (P<0.01); CYR1 expression decreased in the biofilm at 6, 24 and 36 h, and increased in the biofilm at 12 h (P<0.01); PDE2 expression increased in the 12 h biofilm (P<0.01). ③Compared with the wild strain, the high expression strain of RAS1 gene showed higher SMIC50 in the biofilm at 12 and 24 h, and significantly higher expression of RAS1 gene in the biofilm at 12, 24 and 36 h (P<0.01). ④After treatment with farnesol, the resistance of wild-type strains and high expression strains of RAS1 gene decreased. Compared with the untreated group, the expression of RAS1 gene in the biofilm of wild-type and RAS1 gene high expression strain decreased at 12 and 24 h (P<0.01). Conclusion Farnesol can affect the sensitivity of C. albicans biofilm to fluconazole by regulating the expression of resistance molecules RAS1, CYR1 and PDE2 in the cAMP-PKA pathway. The regulatory effect varies at different stages of biofilm formation. |
| format | Article |
| id | doaj-art-1f5d448030eb4ad281dd98f592de54fd |
| institution | OA Journals |
| issn | 1003-9872 |
| language | zho |
| publishDate | 2025-05-01 |
| publisher | Editorial Office of Stomatology |
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| series | Kouqiang yixue |
| spelling | doaj-art-1f5d448030eb4ad281dd98f592de54fd2025-08-20T02:36:03ZzhoEditorial Office of StomatologyKouqiang yixue1003-98722025-05-0145532132710.13591/j.cnki.kqyx.2025.05.001The effect of farnesol on the cAMP-PKA signaling pathway of Candida albicans biofilms and its correlation with drug resistanceWANG Yang, CHEN Xueyi, YU Shenjun, CAO Xuejiao, WEI Xin, YANG Xuan0Department of Conservative Dentistry and Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing 210029, ChinaObjective To explore the regulatory role of farnesol in Candida albicans (C. albicans) biofilm cAMP-PKA signaling pathway and its correlation with drug resistance. Methods Standard, fluconazole-resistant, wild and high RAS1 gene expression strains of C. albicans were cultured to different phases of the biofilm (6,12,24,36 h), and the sessile minimal inhibitory concentration 50%(SMIC50) of fluconazole were determined by XTT reduction after farnesol treatment. The regulatory effects of farnesol on the expression of genes related to the cAMP-PKA signaling pathway in standard and fluconazole-resistant strains of C. albicans, such as RAS1, CYR1, PDE2 were examined using qPCR; the effects of farnesol on the protein expression of the pathway were analyzed by Western blot. RAS1 gene expression of the wild and high RAS1 gene expression strains was measured by qPCR. Results ① Compared with the standard strain, resistant strains of C. albicans had higher levels of biofilm SMIC50 at 6, 12 and 24 h; there was no significant difference in RAS1 expression (P>0.05), while CYR1 expression increased significantly at 6 and 24 h in the biofilm (P<0.01), and PDE2 expression decreased at 6 h in the biofilm (P<0.01). ②After treatment with farnesol, the resistance of the biofilm of the standard strain and drug-resistant strain decreased. Compared with no treatment with farnesol, the expression of RAS1 in the biofilm of the standard strain and drug-resistant strain decreased at all time points (P<0.01); CYR1 expression decreased in the biofilm at 6, 24 and 36 h, and increased in the biofilm at 12 h (P<0.01); PDE2 expression increased in the 12 h biofilm (P<0.01). ③Compared with the wild strain, the high expression strain of RAS1 gene showed higher SMIC50 in the biofilm at 12 and 24 h, and significantly higher expression of RAS1 gene in the biofilm at 12, 24 and 36 h (P<0.01). ④After treatment with farnesol, the resistance of wild-type strains and high expression strains of RAS1 gene decreased. Compared with the untreated group, the expression of RAS1 gene in the biofilm of wild-type and RAS1 gene high expression strain decreased at 12 and 24 h (P<0.01). Conclusion Farnesol can affect the sensitivity of C. albicans biofilm to fluconazole by regulating the expression of resistance molecules RAS1, CYR1 and PDE2 in the cAMP-PKA pathway. The regulatory effect varies at different stages of biofilm formation.https://www.stomatology.cn/fileup/1003-9872/PDF/1747804476736-1736667940.pdf|candida albicans|biofilm|farnesol|camp-pka|drug resistance |
| spellingShingle | WANG Yang, CHEN Xueyi, YU Shenjun, CAO Xuejiao, WEI Xin, YANG Xuan The effect of farnesol on the cAMP-PKA signaling pathway of Candida albicans biofilms and its correlation with drug resistance Kouqiang yixue |candida albicans|biofilm|farnesol|camp-pka|drug resistance |
| title | The effect of farnesol on the cAMP-PKA signaling pathway of Candida albicans biofilms and its correlation with drug resistance |
| title_full | The effect of farnesol on the cAMP-PKA signaling pathway of Candida albicans biofilms and its correlation with drug resistance |
| title_fullStr | The effect of farnesol on the cAMP-PKA signaling pathway of Candida albicans biofilms and its correlation with drug resistance |
| title_full_unstemmed | The effect of farnesol on the cAMP-PKA signaling pathway of Candida albicans biofilms and its correlation with drug resistance |
| title_short | The effect of farnesol on the cAMP-PKA signaling pathway of Candida albicans biofilms and its correlation with drug resistance |
| title_sort | effect of farnesol on the camp pka signaling pathway of candida albicans biofilms and its correlation with drug resistance |
| topic | |candida albicans|biofilm|farnesol|camp-pka|drug resistance |
| url | https://www.stomatology.cn/fileup/1003-9872/PDF/1747804476736-1736667940.pdf |
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