In Vitro Biofilm Formation on Aryl Ketone Polymer (AKP), A New Denture Material, Compared with That on Three Traditional Dental Denture Materials
Control of denture plaque biofilms is a practical approach to preventing persistent oral infections such as denture stomatitis. Objectives. This study compared in vitro biofilm attachment and growth on a new denture material, Ultaire® AKP, with that on traditional denture materials including cobalt...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2021/4713510 |
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| author | Cadie Martin Laura Purevdorj-Gage Wei Li Timothy J. Shary Bin Yang Ryan J. Murphy Christine D. Wu |
| author_facet | Cadie Martin Laura Purevdorj-Gage Wei Li Timothy J. Shary Bin Yang Ryan J. Murphy Christine D. Wu |
| author_sort | Cadie Martin |
| collection | DOAJ |
| description | Control of denture plaque biofilms is a practical approach to preventing persistent oral infections such as denture stomatitis. Objectives. This study compared in vitro biofilm attachment and growth on a new denture material, Ultaire® AKP, with that on traditional denture materials including cobalt chrome (CoCr), polymethyl methacrylate (PMMA), and polyoxymethylene (POM). Methods. Microbial biofilms were grown with cultures of Candida albicans, Streptococcus mutans UA159, or a mixed Streptococcus spp. (S. mutans 700610/Streptococcus sanguinis BAA-1455) for 6 hours in a static protocol or 24 hours in a dynamic protocol for each material. Adherent biofilm cells were removed, and viable colony-forming units (CFUs) were enumerated. Confocal microscopy of the 24-hour Streptococcus spp. biofilms was used to determine biofilm mass and roughness coefficients. Results. The rank order of C. albicans attachment after 6 hours was CoCr > PMMA∗ > Ultaire® AKP∗ (∗vs CoCr, p≤0.05), and that for 24-hour biofilm growth was CoCr > Ultaire® AKP∗ > PMMA∗ (∗vs CoCr, p≤0.05). The rank order of S. mutans biofilm attachment was CoCr > POM > Ultaire® AKP∗ > PMMA∗ (∗vs CoCr, p≤0.05), and that for the 24-hour Streptococcus spp. biofilm growth was POM > Ultaire® AKP > PMMA > CoCr∗ (∗vs POM, p≤0.05). Confocal images revealed structural differences in Streptococcus spp. biofilms on CoCr compared with the other test materials. Significantly lower roughness coefficients of Streptococcus spp. biofilms on Ultaire® AKP were noted, suggesting that these biofilms were less differentiated. Ultaire® AKP promoted significantly less C. albicans and S. mutans biofilm attachment than CoCr at 6 hours and C. albicans growth at 24 hours. Streptococcus spp. biofilms on Ultaire® AKP were less differentiated than those on other test materials. Conclusion. In addition to its material strength, Ultaire® AKP represents an attractive option for denture material in removable partial dentures. |
| format | Article |
| id | doaj-art-1f4fce4669864325969fb4f1b7ce6a7c |
| institution | OA Journals |
| issn | 1687-8736 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-1f4fce4669864325969fb4f1b7ce6a7c2025-08-20T02:04:34ZengWileyInternational Journal of Dentistry1687-87362021-01-01202110.1155/2021/47135104713510In Vitro Biofilm Formation on Aryl Ketone Polymer (AKP), A New Denture Material, Compared with That on Three Traditional Dental Denture MaterialsCadie Martin0Laura Purevdorj-Gage1Wei Li2Timothy J. Shary3Bin Yang4Ryan J. Murphy5Christine D. Wu6Microbiology and Environmental Research & Innovation Center–BristolMicrobiology and Environmental Research & Innovation Center–BristolDepartment of Pediatric DentistrySolvay Specialty PolymersDepartment of Restorative DentistrySolvay Specialty PolymersDepartment of Pediatric DentistryControl of denture plaque biofilms is a practical approach to preventing persistent oral infections such as denture stomatitis. Objectives. This study compared in vitro biofilm attachment and growth on a new denture material, Ultaire® AKP, with that on traditional denture materials including cobalt chrome (CoCr), polymethyl methacrylate (PMMA), and polyoxymethylene (POM). Methods. Microbial biofilms were grown with cultures of Candida albicans, Streptococcus mutans UA159, or a mixed Streptococcus spp. (S. mutans 700610/Streptococcus sanguinis BAA-1455) for 6 hours in a static protocol or 24 hours in a dynamic protocol for each material. Adherent biofilm cells were removed, and viable colony-forming units (CFUs) were enumerated. Confocal microscopy of the 24-hour Streptococcus spp. biofilms was used to determine biofilm mass and roughness coefficients. Results. The rank order of C. albicans attachment after 6 hours was CoCr > PMMA∗ > Ultaire® AKP∗ (∗vs CoCr, p≤0.05), and that for 24-hour biofilm growth was CoCr > Ultaire® AKP∗ > PMMA∗ (∗vs CoCr, p≤0.05). The rank order of S. mutans biofilm attachment was CoCr > POM > Ultaire® AKP∗ > PMMA∗ (∗vs CoCr, p≤0.05), and that for the 24-hour Streptococcus spp. biofilm growth was POM > Ultaire® AKP > PMMA > CoCr∗ (∗vs POM, p≤0.05). Confocal images revealed structural differences in Streptococcus spp. biofilms on CoCr compared with the other test materials. Significantly lower roughness coefficients of Streptococcus spp. biofilms on Ultaire® AKP were noted, suggesting that these biofilms were less differentiated. Ultaire® AKP promoted significantly less C. albicans and S. mutans biofilm attachment than CoCr at 6 hours and C. albicans growth at 24 hours. Streptococcus spp. biofilms on Ultaire® AKP were less differentiated than those on other test materials. Conclusion. In addition to its material strength, Ultaire® AKP represents an attractive option for denture material in removable partial dentures.http://dx.doi.org/10.1155/2021/4713510 |
| spellingShingle | Cadie Martin Laura Purevdorj-Gage Wei Li Timothy J. Shary Bin Yang Ryan J. Murphy Christine D. Wu In Vitro Biofilm Formation on Aryl Ketone Polymer (AKP), A New Denture Material, Compared with That on Three Traditional Dental Denture Materials International Journal of Dentistry |
| title | In Vitro Biofilm Formation on Aryl Ketone Polymer (AKP), A New Denture Material, Compared with That on Three Traditional Dental Denture Materials |
| title_full | In Vitro Biofilm Formation on Aryl Ketone Polymer (AKP), A New Denture Material, Compared with That on Three Traditional Dental Denture Materials |
| title_fullStr | In Vitro Biofilm Formation on Aryl Ketone Polymer (AKP), A New Denture Material, Compared with That on Three Traditional Dental Denture Materials |
| title_full_unstemmed | In Vitro Biofilm Formation on Aryl Ketone Polymer (AKP), A New Denture Material, Compared with That on Three Traditional Dental Denture Materials |
| title_short | In Vitro Biofilm Formation on Aryl Ketone Polymer (AKP), A New Denture Material, Compared with That on Three Traditional Dental Denture Materials |
| title_sort | in vitro biofilm formation on aryl ketone polymer akp a new denture material compared with that on three traditional dental denture materials |
| url | http://dx.doi.org/10.1155/2021/4713510 |
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