Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo
Abstract The microinjection of Zebrafish embryos is significant to life science and biomedical research. In this article, a novel automated system is developed for cell microinjection. A sophisticated microfluidic chip is designed to transport, hold, and inject cells continuously. For the first time...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-02-01
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| Series: | Microsystems & Nanoengineering |
| Online Access: | https://doi.org/10.1038/s41378-024-00809-y |
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| _version_ | 1849723856001957888 |
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| author | Xiangyu Guo Antian Zhao Youchao Zhang Huanyu Jiang Longhua Tang Bo Lu Yibin Ying Mingchuan Zhou |
| author_facet | Xiangyu Guo Antian Zhao Youchao Zhang Huanyu Jiang Longhua Tang Bo Lu Yibin Ying Mingchuan Zhou |
| author_sort | Xiangyu Guo |
| collection | DOAJ |
| description | Abstract The microinjection of Zebrafish embryos is significant to life science and biomedical research. In this article, a novel automated system is developed for cell microinjection. A sophisticated microfluidic chip is designed to transport, hold, and inject cells continuously. For the first time, a microinjector with microforce perception is proposed and integrated within the enclosed microfluidic chip to judge whether cells have been successfully punctured. The deep learning model is employed to detect the yolk center of zebrafish embryos and locate the position of the injection needle within the yolk, which enables enhancing the precision of cell injection. A prototype is fabricated to achieve automatic batch microinjection. Experimental results demonstrated that the injection efficiency is about 20 seconds per cell. Cell puncture success rate and cell survival rate are 100% and 84%, respectively. Compared to manual operation, this proposed system improves cell operation efficiency and cell survival rate. The proposed microinjection system has the potential to greatly reduce the workload of the experimenters and shorten the relevant study period. |
| format | Article |
| id | doaj-art-1f4a58f6394a46d4877a711ea3d188cc |
| institution | DOAJ |
| issn | 2055-7434 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Microsystems & Nanoengineering |
| spelling | doaj-art-1f4a58f6394a46d4877a711ea3d188cc2025-08-20T03:10:54ZengNature Publishing GroupMicrosystems & Nanoengineering2055-74342025-02-0111111310.1038/s41378-024-00809-yDesign and developing a robot-assisted cell batch microinjection system for zebrafish embryoXiangyu Guo0Antian Zhao1Youchao Zhang2Huanyu Jiang3Longhua Tang4Bo Lu5Yibin Ying6Mingchuan Zhou7Robotic Micro-nano Manipulation Lab, College of Biosystems Engineering and Food Science, Zhejiang UniversityRobotic Micro-nano Manipulation Lab, College of Biosystems Engineering and Food Science, Zhejiang UniversityRobotic Micro-nano Manipulation Lab, College of Biosystems Engineering and Food Science, Zhejiang UniversityRobotic Micro-nano Manipulation Lab, College of Biosystems Engineering and Food Science, Zhejiang UniversityCollege of Optical Science and Engineering, Zhejiang UniversityRobotics and Microsystems Center, School of Mechanical and Electric Engineering, Soochow UniversityRobotic Micro-nano Manipulation Lab, College of Biosystems Engineering and Food Science, Zhejiang UniversityRobotic Micro-nano Manipulation Lab, College of Biosystems Engineering and Food Science, Zhejiang UniversityAbstract The microinjection of Zebrafish embryos is significant to life science and biomedical research. In this article, a novel automated system is developed for cell microinjection. A sophisticated microfluidic chip is designed to transport, hold, and inject cells continuously. For the first time, a microinjector with microforce perception is proposed and integrated within the enclosed microfluidic chip to judge whether cells have been successfully punctured. The deep learning model is employed to detect the yolk center of zebrafish embryos and locate the position of the injection needle within the yolk, which enables enhancing the precision of cell injection. A prototype is fabricated to achieve automatic batch microinjection. Experimental results demonstrated that the injection efficiency is about 20 seconds per cell. Cell puncture success rate and cell survival rate are 100% and 84%, respectively. Compared to manual operation, this proposed system improves cell operation efficiency and cell survival rate. The proposed microinjection system has the potential to greatly reduce the workload of the experimenters and shorten the relevant study period.https://doi.org/10.1038/s41378-024-00809-y |
| spellingShingle | Xiangyu Guo Antian Zhao Youchao Zhang Huanyu Jiang Longhua Tang Bo Lu Yibin Ying Mingchuan Zhou Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo Microsystems & Nanoengineering |
| title | Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo |
| title_full | Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo |
| title_fullStr | Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo |
| title_full_unstemmed | Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo |
| title_short | Design and developing a robot-assisted cell batch microinjection system for zebrafish embryo |
| title_sort | design and developing a robot assisted cell batch microinjection system for zebrafish embryo |
| url | https://doi.org/10.1038/s41378-024-00809-y |
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