Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection Agents
Visualization and quantification of inflammatory processes is of high importance for early diagnosis of a multitude of diseases. Magnetic resonance imaging (MRI) using iron oxide (FeO) nanoparticles as contrast agents allows the study of macrophage infiltration during inflammation in a variety of ti...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2006-04-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2006.00010 |
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| _version_ | 1832544462641823744 |
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| author | Cedric Berger Martin Rausch Philipp Schmidt Markus Rudin |
| author_facet | Cedric Berger Martin Rausch Philipp Schmidt Markus Rudin |
| author_sort | Cedric Berger |
| collection | DOAJ |
| description | Visualization and quantification of inflammatory processes is of high importance for early diagnosis of a multitude of diseases. Magnetic resonance imaging (MRI) using iron oxide (FeO) nanoparticles as contrast agents allows the study of macrophage infiltration during inflammation in a variety of tissues. Macrophages are effectors of the immune response, their appearance being orchestrated by activated T lymphocytes. Therefore, tracking of labeled T lymphocytes, which initiate the immune process, should enable earlier detection of tissue inflammation. In this study, we investigate the feasibility of specifically labeling harvested T cells by using dextran-coated FeO nanoparticles and commonly available transfection agents (TAs). Physicochemical properties of the newly formed FeO/TA vesicles were determined as well as their cell toxicity and their T cell activation potential. The labeling efficiency of each FeO/TA combination was evaluated by measuring the transverse MRI relaxation rate R 2 by X-ray spectroscopy and magnetic selection. Toxicity and labeling efficacy differed significantly among TAs. The best results were achieved by using polyamine TAs and in particular by using poly- l -lysine at a concentration of 1.5 µg/mL administered in combination with 22.5 µg iron/mL. By using this protocol, up to 60% of harvested T cells could be labeled. Microscopic investigation revealed FeO/TA nanoparticles not only localized within the cytoplasma of the cells but also sticking to the outer membrane surface. |
| format | Article |
| id | doaj-art-1f3fc764c2f34a1798d580fce61225ec |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2006-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-1f3fc764c2f34a1798d580fce61225ec2025-02-03T10:13:00ZengSAGE PublishingMolecular Imaging1536-01212006-04-01510.2310/7290.2006.0001010.2310_7290.2006.00010Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection AgentsCedric Berger0Martin Rausch1Philipp Schmidt2Markus Rudin3Novartis Institutes for Biomedical Research, SwitzerlandNovartis Institutes for Biomedical Research, SwitzerlandNovartis Institutes for Biomedical Research, SwitzerlandUniversity of Zürich & ETH Zürich, SwitzerlandVisualization and quantification of inflammatory processes is of high importance for early diagnosis of a multitude of diseases. Magnetic resonance imaging (MRI) using iron oxide (FeO) nanoparticles as contrast agents allows the study of macrophage infiltration during inflammation in a variety of tissues. Macrophages are effectors of the immune response, their appearance being orchestrated by activated T lymphocytes. Therefore, tracking of labeled T lymphocytes, which initiate the immune process, should enable earlier detection of tissue inflammation. In this study, we investigate the feasibility of specifically labeling harvested T cells by using dextran-coated FeO nanoparticles and commonly available transfection agents (TAs). Physicochemical properties of the newly formed FeO/TA vesicles were determined as well as their cell toxicity and their T cell activation potential. The labeling efficiency of each FeO/TA combination was evaluated by measuring the transverse MRI relaxation rate R 2 by X-ray spectroscopy and magnetic selection. Toxicity and labeling efficacy differed significantly among TAs. The best results were achieved by using polyamine TAs and in particular by using poly- l -lysine at a concentration of 1.5 µg/mL administered in combination with 22.5 µg iron/mL. By using this protocol, up to 60% of harvested T cells could be labeled. Microscopic investigation revealed FeO/TA nanoparticles not only localized within the cytoplasma of the cells but also sticking to the outer membrane surface.https://doi.org/10.2310/7290.2006.00010 |
| spellingShingle | Cedric Berger Martin Rausch Philipp Schmidt Markus Rudin Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection Agents Molecular Imaging |
| title | Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection Agents |
| title_full | Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection Agents |
| title_fullStr | Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection Agents |
| title_full_unstemmed | Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection Agents |
| title_short | Feasibility and Limits of Magnetically Labeling Primary Cultured Rat T Cells with Ferumoxides Coupled with Commonly Used Transfection Agents |
| title_sort | feasibility and limits of magnetically labeling primary cultured rat t cells with ferumoxides coupled with commonly used transfection agents |
| url | https://doi.org/10.2310/7290.2006.00010 |
| work_keys_str_mv | AT cedricberger feasibilityandlimitsofmagneticallylabelingprimaryculturedrattcellswithferumoxidescoupledwithcommonlyusedtransfectionagents AT martinrausch feasibilityandlimitsofmagneticallylabelingprimaryculturedrattcellswithferumoxidescoupledwithcommonlyusedtransfectionagents AT philippschmidt feasibilityandlimitsofmagneticallylabelingprimaryculturedrattcellswithferumoxidescoupledwithcommonlyusedtransfectionagents AT markusrudin feasibilityandlimitsofmagneticallylabelingprimaryculturedrattcellswithferumoxidescoupledwithcommonlyusedtransfectionagents |