Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes
Abstract Natural products are ubiquitously produced by many species that we encounter during our daily lives. One genus, Streptococcus, can produce a wide array of quorum sensing linked natural products known as RaS-RiPPs (ribosomally synthesized and post-translationally modified peptides). Their pr...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-08-01
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| Series: | npj Biofilms and Microbiomes |
| Online Access: | https://doi.org/10.1038/s41522-025-00794-8 |
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| author | Britta E. Rued Achal Dhariwal Brett C. Covington Mohammad R. Seyedsayamdost Sophie A. Krivograd Russell P. Pesavento Michael J. Federle Fernanda C. Petersen |
| author_facet | Britta E. Rued Achal Dhariwal Brett C. Covington Mohammad R. Seyedsayamdost Sophie A. Krivograd Russell P. Pesavento Michael J. Federle Fernanda C. Petersen |
| author_sort | Britta E. Rued |
| collection | DOAJ |
| description | Abstract Natural products are ubiquitously produced by many species that we encounter during our daily lives. One genus, Streptococcus, can produce a wide array of quorum sensing linked natural products known as RaS-RiPPs (ribosomally synthesized and post-translationally modified peptides). Their production is triggered by the induction of an Rgg-SHP quorum sensing system, which senses the presence of SHPs (short hydrophobic peptides) and induces the gene expression of these operons. Previous work has found that streptococcal RaS-RiPPs modulate the growth of different streptococci and might play a role in antibiotic tolerance. This is of particular importance to the oral microbiome, where streptococci are a predominant genus. This study provides the first report on attempts to study the impact of the RaS-RiPP Tryglysin A on ex-vivo oral systems and explores important factors to consider when culturing these systems. We explore how medium selection, atmosphere, growth model, and saliva amount can impact the presence of both bacterial and fungal species. These studies provide the groundwork for determining how RaS-RiPP producing Streptococci might impact the composition and function of oral microbiome communities, as well as important aspects to consider when culturing ex-vivo oral systems. |
| format | Article |
| id | doaj-art-1f36e9877d0741558ea03e1af3867622 |
| institution | Kabale University |
| issn | 2055-5008 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Biofilms and Microbiomes |
| spelling | doaj-art-1f36e9877d0741558ea03e1af38676222025-08-20T04:01:52ZengNature Portfolionpj Biofilms and Microbiomes2055-50082025-08-0111111510.1038/s41522-025-00794-8Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomesBritta E. Rued0Achal Dhariwal1Brett C. Covington2Mohammad R. Seyedsayamdost3Sophie A. Krivograd4Russell P. Pesavento5Michael J. Federle6Fernanda C. Petersen7Department of Veterinary Microbiology and Preventive Medicine, Iowa State UniversityInstitute of Oral Biology, University of OsloDepartment of Chemistry, Princeton UniversityDepartment of Chemistry, Princeton UniversityDepartment of Veterinary Microbiology and Preventive Medicine, Iowa State UniversityDepartment of Oral Biology, College of Dentistry, University of IllinoisDepartment of Pharmaceutical Sciences, University of IllinoisInstitute of Oral Biology, University of OsloAbstract Natural products are ubiquitously produced by many species that we encounter during our daily lives. One genus, Streptococcus, can produce a wide array of quorum sensing linked natural products known as RaS-RiPPs (ribosomally synthesized and post-translationally modified peptides). Their production is triggered by the induction of an Rgg-SHP quorum sensing system, which senses the presence of SHPs (short hydrophobic peptides) and induces the gene expression of these operons. Previous work has found that streptococcal RaS-RiPPs modulate the growth of different streptococci and might play a role in antibiotic tolerance. This is of particular importance to the oral microbiome, where streptococci are a predominant genus. This study provides the first report on attempts to study the impact of the RaS-RiPP Tryglysin A on ex-vivo oral systems and explores important factors to consider when culturing these systems. We explore how medium selection, atmosphere, growth model, and saliva amount can impact the presence of both bacterial and fungal species. These studies provide the groundwork for determining how RaS-RiPP producing Streptococci might impact the composition and function of oral microbiome communities, as well as important aspects to consider when culturing ex-vivo oral systems.https://doi.org/10.1038/s41522-025-00794-8 |
| spellingShingle | Britta E. Rued Achal Dhariwal Brett C. Covington Mohammad R. Seyedsayamdost Sophie A. Krivograd Russell P. Pesavento Michael J. Federle Fernanda C. Petersen Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes npj Biofilms and Microbiomes |
| title | Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes |
| title_full | Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes |
| title_fullStr | Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes |
| title_full_unstemmed | Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes |
| title_short | Impact of the RaS-RiPP tryglysin and culturing conditions on ex-vivo oral microbiomes |
| title_sort | impact of the ras ripp tryglysin and culturing conditions on ex vivo oral microbiomes |
| url | https://doi.org/10.1038/s41522-025-00794-8 |
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