Expression patterns and clinical value of key m6A RNA modification regulators in smoking patients with coronary artery disease
Even though N6-methyladenosine (m6A) RNA modifications are increasingly being implicated in human disease, their mechanisms are not fully understood in smokers with coronary artery disease (CAD). Thirty m6A-related regulators’ expression (MRRE) in CAD individuals (smokers and non-smokers) were analy...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Epigenetics |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15592294.2024.2392400 |
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| author | Huanwei Zhuang Hua Ouyang Yangfei Peng Shuji Gong Kun Xiang Le Chen Jinlan Chen |
| author_facet | Huanwei Zhuang Hua Ouyang Yangfei Peng Shuji Gong Kun Xiang Le Chen Jinlan Chen |
| author_sort | Huanwei Zhuang |
| collection | DOAJ |
| description | Even though N6-methyladenosine (m6A) RNA modifications are increasingly being implicated in human disease, their mechanisms are not fully understood in smokers with coronary artery disease (CAD). Thirty m6A-related regulators’ expression (MRRE) in CAD individuals (smokers and non-smokers) were analyzed from GEO. Support Vector Machine, random forest, and nomogram models were constructed to assess its clinical value. Consensus clustering, principal component analysis, and ssGSEA were used to construct a full picture of m6A-related regulators in smokers with CAD. Oxygen-glucose deprivation (OGD) and qRT-PCR were used to validate hypoxia’s effect on MRRE. A comparison between smokers with CAD and controls revealed lower expression levels of RBM15B, YTHDC2, and ZC3H13. Based on three key MRREs, all models showed good clinical value, and smokers with CAD were divided into two distinct molecular subgroups. The correlations were found between key MRRE and the degree of immune infiltration. Three key MRREs in HUVECs and FMC84 mouse cardiomyocytes were reduced in the OGD group. Through hypoxia, smoking might reduce the expression levels of RBM15B, YTHDC2, and ZC3H13 in smokers with CAD. Our findings provide an important theoretical basis for the treatment of smokers with CAD. |
| format | Article |
| id | doaj-art-1f33ffe4f3524164a0741001329ed497 |
| institution | OA Journals |
| issn | 1559-2294 1559-2308 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj-art-1f33ffe4f3524164a0741001329ed4972025-08-20T02:20:49ZengTaylor & Francis GroupEpigenetics1559-22941559-23082024-12-0119110.1080/15592294.2024.2392400Expression patterns and clinical value of key m6A RNA modification regulators in smoking patients with coronary artery diseaseHuanwei Zhuang0Hua Ouyang1Yangfei Peng2Shuji Gong3Kun Xiang4Le Chen5Jinlan Chen6Department of Cardiovascular Surgery, Haikou Affiliated Hospital of Central South University Xiangya School of Medicine, Haikou, ChinaDepartment of Thoracic Surgery, ZhuJiang Hospital of Southern Medical University, Southern Medical University, Guangzhou, ChinaDepartment of Thoracic Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, ChinaDepartment of Cardiovascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Cardiovascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Cardiovascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, ChinaDepartment of Cardiovascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, ChinaEven though N6-methyladenosine (m6A) RNA modifications are increasingly being implicated in human disease, their mechanisms are not fully understood in smokers with coronary artery disease (CAD). Thirty m6A-related regulators’ expression (MRRE) in CAD individuals (smokers and non-smokers) were analyzed from GEO. Support Vector Machine, random forest, and nomogram models were constructed to assess its clinical value. Consensus clustering, principal component analysis, and ssGSEA were used to construct a full picture of m6A-related regulators in smokers with CAD. Oxygen-glucose deprivation (OGD) and qRT-PCR were used to validate hypoxia’s effect on MRRE. A comparison between smokers with CAD and controls revealed lower expression levels of RBM15B, YTHDC2, and ZC3H13. Based on three key MRREs, all models showed good clinical value, and smokers with CAD were divided into two distinct molecular subgroups. The correlations were found between key MRRE and the degree of immune infiltration. Three key MRREs in HUVECs and FMC84 mouse cardiomyocytes were reduced in the OGD group. Through hypoxia, smoking might reduce the expression levels of RBM15B, YTHDC2, and ZC3H13 in smokers with CAD. Our findings provide an important theoretical basis for the treatment of smokers with CAD.https://www.tandfonline.com/doi/10.1080/15592294.2024.2392400Coronary artery diseasesmokingN6-methyladenosine RNA modificationhypoxiaepigenetic modification |
| spellingShingle | Huanwei Zhuang Hua Ouyang Yangfei Peng Shuji Gong Kun Xiang Le Chen Jinlan Chen Expression patterns and clinical value of key m6A RNA modification regulators in smoking patients with coronary artery disease Epigenetics Coronary artery disease smoking N6-methyladenosine RNA modification hypoxia epigenetic modification |
| title | Expression patterns and clinical value of key m6A RNA modification regulators in smoking patients with coronary artery disease |
| title_full | Expression patterns and clinical value of key m6A RNA modification regulators in smoking patients with coronary artery disease |
| title_fullStr | Expression patterns and clinical value of key m6A RNA modification regulators in smoking patients with coronary artery disease |
| title_full_unstemmed | Expression patterns and clinical value of key m6A RNA modification regulators in smoking patients with coronary artery disease |
| title_short | Expression patterns and clinical value of key m6A RNA modification regulators in smoking patients with coronary artery disease |
| title_sort | expression patterns and clinical value of key m6a rna modification regulators in smoking patients with coronary artery disease |
| topic | Coronary artery disease smoking N6-methyladenosine RNA modification hypoxia epigenetic modification |
| url | https://www.tandfonline.com/doi/10.1080/15592294.2024.2392400 |
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