An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization
Yarrowia lipolytica, a safe yeast, efficiently metabolizes lipids for the production of food additives and agricultural products. Boosting its growth and lipid utilization capabilities is crucial to enhancing the overall efficiency Y. lipolytica. Herein, an integrated strategy was implemented to enh...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-06-01
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| Series: | Synthetic and Systems Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X25000079 |
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| author | Tianyu Dong Yujie Shu Ying Wang Mingdong Yao Wenhai Xiao |
| author_facet | Tianyu Dong Yujie Shu Ying Wang Mingdong Yao Wenhai Xiao |
| author_sort | Tianyu Dong |
| collection | DOAJ |
| description | Yarrowia lipolytica, a safe yeast, efficiently metabolizes lipids for the production of food additives and agricultural products. Boosting its growth and lipid utilization capabilities is crucial to enhancing the overall efficiency Y. lipolytica. Herein, an integrated strategy was implemented to enhance lipid uptake, accumulation and metabolism and systematically promote the growth and lipid utilization of the commonly used Y. lipolytica Po1f strain. The engineered strain had a specific growth rate of 0.32 h−1 and a lipid content of 67.66 % (g/g DCW), which were 54 % and 26 % greater than those of the original strain. β-Carotene was used to verify the production of lipophilic natural compounds, and the highest yield was obtained 48 h earlier using the engineered strain compared to the original strain when consuming same carbon source. These findings show promise in using engineered Y. lipolytica for rapid growth and improved lipid utilization to boost efficiency of lipophilic product production. |
| format | Article |
| id | doaj-art-1f25ffec4e4a4f90bca20f26c9bd385a |
| institution | Kabale University |
| issn | 2405-805X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj-art-1f25ffec4e4a4f90bca20f26c9bd385a2025-02-09T05:00:38ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2025-06-01102495503An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilizationTianyu Dong0Yujie Shu1Ying Wang2Mingdong Yao3Wenhai Xiao4Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; Frontier Research Institute for Synthetic Biology, Tianjin University, Tianjin, 300072, ChinaFrontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; Frontier Research Institute for Synthetic Biology, Tianjin University, Tianjin, 300072, ChinaFrontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; Frontier Research Institute for Synthetic Biology, Tianjin University, Tianjin, 300072, ChinaFrontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; Frontier Research Institute for Synthetic Biology, Tianjin University, Tianjin, 300072, China; Corresponding author. Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; School of Life Science, Faculty of Medicine, Tianjin University, Tianjin, 300072, China; Frontier Research Institute for Synthetic Biology, Tianjin University, Tianjin, 300072, China; Georgia Tech Shenzhen Institute, Tianjin University, Shenzhen, 518071, China; Corresponding author. Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.Yarrowia lipolytica, a safe yeast, efficiently metabolizes lipids for the production of food additives and agricultural products. Boosting its growth and lipid utilization capabilities is crucial to enhancing the overall efficiency Y. lipolytica. Herein, an integrated strategy was implemented to enhance lipid uptake, accumulation and metabolism and systematically promote the growth and lipid utilization of the commonly used Y. lipolytica Po1f strain. The engineered strain had a specific growth rate of 0.32 h−1 and a lipid content of 67.66 % (g/g DCW), which were 54 % and 26 % greater than those of the original strain. β-Carotene was used to verify the production of lipophilic natural compounds, and the highest yield was obtained 48 h earlier using the engineered strain compared to the original strain when consuming same carbon source. These findings show promise in using engineered Y. lipolytica for rapid growth and improved lipid utilization to boost efficiency of lipophilic product production.http://www.sciencedirect.com/science/article/pii/S2405805X25000079Yarrowia lipolyticaCell growthLipid accumulationAcetyl-CoA accumulationβ-carotene |
| spellingShingle | Tianyu Dong Yujie Shu Ying Wang Mingdong Yao Wenhai Xiao An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization Synthetic and Systems Biotechnology Yarrowia lipolytica Cell growth Lipid accumulation Acetyl-CoA accumulation β-carotene |
| title | An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization |
| title_full | An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization |
| title_fullStr | An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization |
| title_full_unstemmed | An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization |
| title_short | An engineered Yarrowia lipolytica with rapid growth and efficient lipid utilization |
| title_sort | engineered yarrowia lipolytica with rapid growth and efficient lipid utilization |
| topic | Yarrowia lipolytica Cell growth Lipid accumulation Acetyl-CoA accumulation β-carotene |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X25000079 |
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