The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions
ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is the leading cause of wound infections, often progressing into serious invasive bloodstream infections. MRSA disproportionately affects Indigenous peoples in Canada with higher rates of skin and wound infections, an example of persistent...
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American Society for Microbiology
2024-12-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02341-24 |
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| author | Colin D. Rieger Ahmed M. Soliman Kateryna Kaplia Nilrup Ghosh Alexa Cervantes Lopez Surya Arcot Venkatesan Abraham Gildaro Guevara Flores Matheus Antônio Filiol Belin Florence Allen Margaret Reynolds Betty McKenna Harold Lavallee Archie Weenie Thomas Favel Fidji Gendron Vincent E. Ziffle Omar M. El-Halfawy |
| author_facet | Colin D. Rieger Ahmed M. Soliman Kateryna Kaplia Nilrup Ghosh Alexa Cervantes Lopez Surya Arcot Venkatesan Abraham Gildaro Guevara Flores Matheus Antônio Filiol Belin Florence Allen Margaret Reynolds Betty McKenna Harold Lavallee Archie Weenie Thomas Favel Fidji Gendron Vincent E. Ziffle Omar M. El-Halfawy |
| author_sort | Colin D. Rieger |
| collection | DOAJ |
| description | ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is the leading cause of wound infections, often progressing into serious invasive bloodstream infections. MRSA disproportionately affects Indigenous peoples in Canada with higher rates of skin and wound infections, an example of persistent gaps in health outcomes between Indigenous and non-Indigenous peoples precipitated by the legacy of colonialism. Conversely, Indigenous peoples have long used natural remedies for infections and other diseases; however, their knowledge was rarely considered for modern medicine. The stagnant antibiotic discovery pipeline and alarming rise of resistance to current antibiotics prompted us to turn to Indigenous medicine as an untapped source of antimicrobials. As such, we collected and prepared 85 extracts of medicinal plants of value to Indigenous peoples spanning the Canadian Prairies. We explored the antimicrobial potential of these extracts against MRSA under wound infection-mimetic conditions compared with culture media typically used to study bacterial antibiotic responses and biofilms but not adequately representative of infection sites. We identified extracts with MRSA growth inhibitory [e.g., bergamot, dock, gaillardia, and dandelion extracts] and biofilm prevention and eradication [e.g., gumweed extracts] activities. Extracts, including those of chokecherry, hoary puccoon, and Northern bedstraw, were only active under wound infection-mimetic conditions, highlighting the benefit of antibiotic discovery under host-relevant conditions. Testing growth inhibitory extracts against an S. aureus cross-resistance platform suggested that they act through mechanisms likely distinct from known antibiotic classes. Together, through an interdisciplinary partnership leveraging Western approaches and traditional Indigenous knowledge, we identified plant extracts with promising antimicrobial potential for drug-resistant MRSA wound infections.IMPORTANCEWe explored the antimicrobial potential of traditional Indigenous remedies against MRSA under wound infection-mimetic conditions. We chose to tackle MRSA wound infections because they constitute an Indigenous health priority, ensuring mutual benefits and reciprocity, which are important principles in partnerships between Indigenous and non-Indigenous researchers. Our partnerships strive to serve as steps towards reconciliation with Indigenous peoples in Canada and a roadmap inspiring similar interdisciplinary collaborations to tackle other healthcare priorities. We identified extracts with promising antibacterial growth inhibitory, biofilm prevention, and eradication activities against MRSA. The antimicrobial potential of some extracts was only observed under wound infection-mimetic conditions, a proof-of-concept that screening under infection-mimetic conditions reveals novel activity undetected under standard conditions. The natural product antimicrobial extracts discovered herein warrant further investigation into their mode of action and chemical composition; they may address the dire need for new antimicrobial and anti-biofilm activity to counter the AMR crisis. |
| format | Article |
| id | doaj-art-1f2408f166cf4dfa951f6809deab1bd5 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | American Society for Microbiology |
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| series | Microbiology Spectrum |
| spelling | doaj-art-1f2408f166cf4dfa951f6809deab1bd52024-12-05T14:01:23ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-12-01121210.1128/spectrum.02341-24The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditionsColin D. Rieger0Ahmed M. Soliman1Kateryna Kaplia2Nilrup Ghosh3Alexa Cervantes Lopez4Surya Arcot Venkatesan5Abraham Gildaro Guevara Flores6Matheus Antônio Filiol Belin7Florence Allen8Margaret Reynolds9Betty McKenna10Harold Lavallee11Archie Weenie12Thomas Favel13Fidji Gendron14Vincent E. Ziffle15Omar M. El-Halfawy16Department of Chemistry and Biochemistry, Faculty of Science, University of Regina, Regina, CanadaDepartment of Chemistry and Biochemistry, Faculty of Science, University of Regina, Regina, CanadaDepartment of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada, Regina, CanadaDepartment of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada, Regina, CanadaDepartment of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada, Regina, CanadaDepartment of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada, Regina, CanadaDepartment of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada, Regina, CanadaDepartment of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada, Regina, CanadaElder from Peter Ballantyne Cree Nation in Saskatchewan, Treaty 6 Territory, Saskatchewan, CanadaElder from English River First Nation in Saskatchewan, Treaty 10 Territory, Saskatchewan, CanadaElder from Shoal River Band in Manitoba, Treaty 4 Territory, Saskatchewan, CanadaElder from Piapot First Nation in Saskatchewan, Treaty 4 Territory, Saskatchewan, CanadaElder from Sweetgrass First Nation in Saskatchewan, Treaty 6 Territory, Saskatchewan, CanadaElder from Kawacatoose First Nation in Saskatchewan, Treaty 4 Territory, Saskatchewan, CanadaDepartment of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada, Regina, CanadaDepartment of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada, Regina, CanadaDepartment of Chemistry and Biochemistry, Faculty of Science, University of Regina, Regina, CanadaABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is the leading cause of wound infections, often progressing into serious invasive bloodstream infections. MRSA disproportionately affects Indigenous peoples in Canada with higher rates of skin and wound infections, an example of persistent gaps in health outcomes between Indigenous and non-Indigenous peoples precipitated by the legacy of colonialism. Conversely, Indigenous peoples have long used natural remedies for infections and other diseases; however, their knowledge was rarely considered for modern medicine. The stagnant antibiotic discovery pipeline and alarming rise of resistance to current antibiotics prompted us to turn to Indigenous medicine as an untapped source of antimicrobials. As such, we collected and prepared 85 extracts of medicinal plants of value to Indigenous peoples spanning the Canadian Prairies. We explored the antimicrobial potential of these extracts against MRSA under wound infection-mimetic conditions compared with culture media typically used to study bacterial antibiotic responses and biofilms but not adequately representative of infection sites. We identified extracts with MRSA growth inhibitory [e.g., bergamot, dock, gaillardia, and dandelion extracts] and biofilm prevention and eradication [e.g., gumweed extracts] activities. Extracts, including those of chokecherry, hoary puccoon, and Northern bedstraw, were only active under wound infection-mimetic conditions, highlighting the benefit of antibiotic discovery under host-relevant conditions. Testing growth inhibitory extracts against an S. aureus cross-resistance platform suggested that they act through mechanisms likely distinct from known antibiotic classes. Together, through an interdisciplinary partnership leveraging Western approaches and traditional Indigenous knowledge, we identified plant extracts with promising antimicrobial potential for drug-resistant MRSA wound infections.IMPORTANCEWe explored the antimicrobial potential of traditional Indigenous remedies against MRSA under wound infection-mimetic conditions. We chose to tackle MRSA wound infections because they constitute an Indigenous health priority, ensuring mutual benefits and reciprocity, which are important principles in partnerships between Indigenous and non-Indigenous researchers. Our partnerships strive to serve as steps towards reconciliation with Indigenous peoples in Canada and a roadmap inspiring similar interdisciplinary collaborations to tackle other healthcare priorities. We identified extracts with promising antibacterial growth inhibitory, biofilm prevention, and eradication activities against MRSA. The antimicrobial potential of some extracts was only observed under wound infection-mimetic conditions, a proof-of-concept that screening under infection-mimetic conditions reveals novel activity undetected under standard conditions. The natural product antimicrobial extracts discovered herein warrant further investigation into their mode of action and chemical composition; they may address the dire need for new antimicrobial and anti-biofilm activity to counter the AMR crisis.https://journals.asm.org/doi/10.1128/spectrum.02341-24wound infection-mimetic conditionsMRSAIndigenous remediesbiofilm preventionbiofilm eradication |
| spellingShingle | Colin D. Rieger Ahmed M. Soliman Kateryna Kaplia Nilrup Ghosh Alexa Cervantes Lopez Surya Arcot Venkatesan Abraham Gildaro Guevara Flores Matheus Antônio Filiol Belin Florence Allen Margaret Reynolds Betty McKenna Harold Lavallee Archie Weenie Thomas Favel Fidji Gendron Vincent E. Ziffle Omar M. El-Halfawy The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions Microbiology Spectrum wound infection-mimetic conditions MRSA Indigenous remedies biofilm prevention biofilm eradication |
| title | The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions |
| title_full | The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions |
| title_fullStr | The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions |
| title_full_unstemmed | The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions |
| title_short | The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions |
| title_sort | antimicrobial potential of traditional remedies of indigenous peoples from canada against mrsa planktonic and biofilm bacteria in wound infection mimetic conditions |
| topic | wound infection-mimetic conditions MRSA Indigenous remedies biofilm prevention biofilm eradication |
| url | https://journals.asm.org/doi/10.1128/spectrum.02341-24 |
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