Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial Isolates
Jingjia Zhang,1,2,* Xue Li,1,* Xinmiao Jia,3,* Yun Li,4 Bo Zheng,4 Yunsong Yu,5 Fupin Hu,6 Ziyong Sun,7 Zhongju Chen,7 Ge Zhang,1 Wei Kang,1 Tong Wang,1 Jin Li,1 Haotian Gao,1 Qiaolian Yi,1 Wei Yu,1,8 Xiaobing Chu,1,8 Yingchun Xu,1 Qiwen Yang1,9 1Department of Clinical Labora...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-04-01
|
| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/establishing-sitafloxacin-epidemiological-cut-off-values-ecoffs-for-cl-peer-reviewed-fulltext-article-IDR |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850171392199229440 |
|---|---|
| author | Zhang J Li X Jia X Li Y Zheng B Yu Y Hu F Sun Z Chen Z Zhang G Kang W Wang T Li J Gao H Yi Q Yu W Chu X Xu Y Yang Q |
| author_facet | Zhang J Li X Jia X Li Y Zheng B Yu Y Hu F Sun Z Chen Z Zhang G Kang W Wang T Li J Gao H Yi Q Yu W Chu X Xu Y Yang Q |
| author_sort | Zhang J |
| collection | DOAJ |
| description | Jingjia Zhang,1,2,* Xue Li,1,* Xinmiao Jia,3,* Yun Li,4 Bo Zheng,4 Yunsong Yu,5 Fupin Hu,6 Ziyong Sun,7 Zhongju Chen,7 Ge Zhang,1 Wei Kang,1 Tong Wang,1 Jin Li,1 Haotian Gao,1 Qiaolian Yi,1 Wei Yu,1,8 Xiaobing Chu,1,8 Yingchun Xu,1 Qiwen Yang1,9 1Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People’s Republic of China; 2Clinical Biobank, Biomedical Engineering Facility of National Infrastructures for Translational Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, Beijing, Peoples Republic of China; 3Department of Medical Research Center, State Key Laboratory of Complex Severe & Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People’s Republic of China; 4Institute of Clinical Pharmacology, Peking University, Beijing, People’s Republic of China; 5Department of Clinical Infectious Diseases, Zhejiang Provincial People’s Hospital, Hangzhou, People’s Republic of China; 6Huashan Hospital of Fudan University, Shanghai, People’s Republic of China; 7Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China; 8Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People’s Republic of China; 9Key Laboratory of Pathogen Infection Prevention and control (Peking Union Medical College), Ministry of Education, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qiwen Yang, Department of Clinical Laboratory, Peking Union Medical College Hospital, Email yangqiwen81@vip.163.comObjective: To establish the epidemiological cut-off values (ECOFFs) of sitafloxacin against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, and Streptococcus pneumoniae.Methods: We collected 2264 clinical isolates from five different labs located in four cities in China. The minimum inhibitory concentrations (MICs) and inhibition zone diameters of sitafloxacin for all isolates were determined by using the broth microdilution method (BMD) and the disk diffusion method according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. MIC ECOFFs were determined using ECOFFinder software, with the 99% calculated ECOFF selected as the initial value. Zone diameter ECOFFs were determined via the visual estimation method. Whole-genome sequencing was performed on E. coli strains exhibiting overlapping MICs between wild-type (WT) and non-wild-type (NWT) groups to analyze resistance mechanisms.Results: Sitafloxacin MICs ranged from 0.002 to 64 mg/L, while inhibition zone diameters ranged from 6 to 45 mm across the nine species. MIC ECOFFs were determined as 0.032, 0.064, 0.125, 0.5, 0.064, 0.125, 0.5, 0.25, and 0.125 mg/L for E. coli, K. pneumoniae, P. mirabilis (tentative ECOFF), P. aeruginosa, A. baumannii, S. aureus, E. faecalis, E. faecium, and S. pneumoniae, respectively. Except for S. pneumoniae, MICs of the other eight species showed a high correlation with zone diameters (|r| > 0.8, P < 0.0001). Consequently, the zone diameter ECOFFs were established as 26, 25, 24, 24, 25, 26, 21, and 22 for E. coli, K. pneumoniae, P. mirabilis, P. aeruginosa, A. baumannii, S. aureus, E. faecalis, and E. faecium, respectively.Conclusion: We established MIC and zone diameter ECOFFs for sitafloxacin against the nine species listed above. The MIC ECOFF for P. mirabilis was classified as tentative. For S. pneumoniae, the correlation between zone diameters and MICs was insufficient to establish a zone diameter ECOFF. Keywords: sitafloxacin, ECOFF, MIC, zone diameter, BMD, disk diffusion |
| format | Article |
| id | doaj-art-1f1762f3142f49f7b24fa06110ae61d7 |
| institution | OA Journals |
| issn | 1178-6973 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj-art-1f1762f3142f49f7b24fa06110ae61d72025-08-20T02:20:17ZengDove Medical PressInfection and Drug Resistance1178-69732025-04-01Volume 1819932004102293Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial IsolatesZhang JLi XJia XLi YZheng BYu YHu FSun ZChen ZZhang GKang WWang TLi JGao HYi QYu WChu XXu YYang QJingjia Zhang,1,2,* Xue Li,1,* Xinmiao Jia,3,* Yun Li,4 Bo Zheng,4 Yunsong Yu,5 Fupin Hu,6 Ziyong Sun,7 Zhongju Chen,7 Ge Zhang,1 Wei Kang,1 Tong Wang,1 Jin Li,1 Haotian Gao,1 Qiaolian Yi,1 Wei Yu,1,8 Xiaobing Chu,1,8 Yingchun Xu,1 Qiwen Yang1,9 1Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People’s Republic of China; 2Clinical Biobank, Biomedical Engineering Facility of National Infrastructures for Translational Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, Beijing, Peoples Republic of China; 3Department of Medical Research Center, State Key Laboratory of Complex Severe & Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People’s Republic of China; 4Institute of Clinical Pharmacology, Peking University, Beijing, People’s Republic of China; 5Department of Clinical Infectious Diseases, Zhejiang Provincial People’s Hospital, Hangzhou, People’s Republic of China; 6Huashan Hospital of Fudan University, Shanghai, People’s Republic of China; 7Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People’s Republic of China; 8Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People’s Republic of China; 9Key Laboratory of Pathogen Infection Prevention and control (Peking Union Medical College), Ministry of Education, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Qiwen Yang, Department of Clinical Laboratory, Peking Union Medical College Hospital, Email yangqiwen81@vip.163.comObjective: To establish the epidemiological cut-off values (ECOFFs) of sitafloxacin against Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, and Streptococcus pneumoniae.Methods: We collected 2264 clinical isolates from five different labs located in four cities in China. The minimum inhibitory concentrations (MICs) and inhibition zone diameters of sitafloxacin for all isolates were determined by using the broth microdilution method (BMD) and the disk diffusion method according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. MIC ECOFFs were determined using ECOFFinder software, with the 99% calculated ECOFF selected as the initial value. Zone diameter ECOFFs were determined via the visual estimation method. Whole-genome sequencing was performed on E. coli strains exhibiting overlapping MICs between wild-type (WT) and non-wild-type (NWT) groups to analyze resistance mechanisms.Results: Sitafloxacin MICs ranged from 0.002 to 64 mg/L, while inhibition zone diameters ranged from 6 to 45 mm across the nine species. MIC ECOFFs were determined as 0.032, 0.064, 0.125, 0.5, 0.064, 0.125, 0.5, 0.25, and 0.125 mg/L for E. coli, K. pneumoniae, P. mirabilis (tentative ECOFF), P. aeruginosa, A. baumannii, S. aureus, E. faecalis, E. faecium, and S. pneumoniae, respectively. Except for S. pneumoniae, MICs of the other eight species showed a high correlation with zone diameters (|r| > 0.8, P < 0.0001). Consequently, the zone diameter ECOFFs were established as 26, 25, 24, 24, 25, 26, 21, and 22 for E. coli, K. pneumoniae, P. mirabilis, P. aeruginosa, A. baumannii, S. aureus, E. faecalis, and E. faecium, respectively.Conclusion: We established MIC and zone diameter ECOFFs for sitafloxacin against the nine species listed above. The MIC ECOFF for P. mirabilis was classified as tentative. For S. pneumoniae, the correlation between zone diameters and MICs was insufficient to establish a zone diameter ECOFF. Keywords: sitafloxacin, ECOFF, MIC, zone diameter, BMD, disk diffusionhttps://www.dovepress.com/establishing-sitafloxacin-epidemiological-cut-off-values-ecoffs-for-cl-peer-reviewed-fulltext-article-IDRsitafloxacinecoffmiczone diameterbmddisk diffusion |
| spellingShingle | Zhang J Li X Jia X Li Y Zheng B Yu Y Hu F Sun Z Chen Z Zhang G Kang W Wang T Li J Gao H Yi Q Yu W Chu X Xu Y Yang Q Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial Isolates Infection and Drug Resistance sitafloxacin ecoff mic zone diameter bmd disk diffusion |
| title | Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial Isolates |
| title_full | Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial Isolates |
| title_fullStr | Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial Isolates |
| title_full_unstemmed | Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial Isolates |
| title_short | Establishing Sitafloxacin Epidemiological Cut-off Values (ECOFFs) for Clinical Bacterial Isolates |
| title_sort | establishing sitafloxacin epidemiological cut off values ecoffs for clinical bacterial isolates |
| topic | sitafloxacin ecoff mic zone diameter bmd disk diffusion |
| url | https://www.dovepress.com/establishing-sitafloxacin-epidemiological-cut-off-values-ecoffs-for-cl-peer-reviewed-fulltext-article-IDR |
| work_keys_str_mv | AT zhangj establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT lix establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT jiax establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT liy establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT zhengb establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT yuy establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT huf establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT sunz establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT chenz establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT zhangg establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT kangw establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT wangt establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT lij establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT gaoh establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT yiq establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT yuw establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT chux establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT xuy establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates AT yangq establishingsitafloxacinepidemiologicalcutoffvaluesecoffsforclinicalbacterialisolates |