Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1
A 2,4-dichlorophenol degrading bacterium strain, designated as GT241-1, was isolated from the soil around drainage area of several factories which produced 2,4-dichlorophenol. It was identified as Pseudomonas sp. Strain GT241-1 can decompose 91% of 2, 4-dichlorophenol with original concentration of...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2001-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2001.03.0269 |
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| author | ZHONG Wen-hui HE Guo-qing ZHENG Ping SUN Ming YU Zi-niu |
| author_facet | ZHONG Wen-hui HE Guo-qing ZHENG Ping SUN Ming YU Zi-niu |
| author_sort | ZHONG Wen-hui |
| collection | DOAJ |
| description | A 2,4-dichlorophenol degrading bacterium strain, designated as GT241-1, was isolated from the soil around drainage area of several factories which produced 2,4-dichlorophenol. It was identified as Pseudomonas sp. Strain GT241-1 can decompose 91% of 2, 4-dichlorophenol with original concentration of 90 mg/L within 48 hours. GC/MS analysis revealed that the strain can degrade 2,4-dichlorophenol completely, without accumulation of intermediate metabolite. Heat treatment at 38℃ made 1.9% of GT241-1 strains lose its 2,4-dichlorophenol degrading ability. Strain GT241-1 has two big plasmids. Southern blot results showed that the 3, 5-dichlorocatechol 1,2-dioxygenase gene of strain GT241-1 locates on its about 11kb EcoRI/XbaI fragment. |
| format | Article |
| id | doaj-art-1efb070a139342d5a34a87f98f3287cf |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2001-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-1efb070a139342d5a34a87f98f3287cf2025-08-20T02:54:50ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552001-05-012726927210.3785/1008-9209.2001.03.026910089209Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1ZHONG Wen-huiHE Guo-qingZHENG PingSUN MingYU Zi-niuA 2,4-dichlorophenol degrading bacterium strain, designated as GT241-1, was isolated from the soil around drainage area of several factories which produced 2,4-dichlorophenol. It was identified as Pseudomonas sp. Strain GT241-1 can decompose 91% of 2, 4-dichlorophenol with original concentration of 90 mg/L within 48 hours. GC/MS analysis revealed that the strain can degrade 2,4-dichlorophenol completely, without accumulation of intermediate metabolite. Heat treatment at 38℃ made 1.9% of GT241-1 strains lose its 2,4-dichlorophenol degrading ability. Strain GT241-1 has two big plasmids. Southern blot results showed that the 3, 5-dichlorocatechol 1,2-dioxygenase gene of strain GT241-1 locates on its about 11kb EcoRI/XbaI fragment.https://www.academax.com/doi/10.3785/1008-9209.2001.03.0269<italic>Pseudomonas</italic>2, 4-dichlorophenol3, 5-dichlorocatechol 1, 2-dioxygenase genegene location |
| spellingShingle | ZHONG Wen-hui HE Guo-qing ZHENG Ping SUN Ming YU Zi-niu Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1 浙江大学学报. 农业与生命科学版 <italic>Pseudomonas</italic> 2, 4-dichlorophenol 3, 5-dichlorocatechol 1, 2-dioxygenase gene gene location |
| title | Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1 |
| title_full | Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1 |
| title_fullStr | Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1 |
| title_full_unstemmed | Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1 |
| title_short | Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1 |
| title_sort | characteristics and 3 5 dichlorocatechol 1 2 dioxygenase gene location of pseudomonas sp gt241 1 |
| topic | <italic>Pseudomonas</italic> 2, 4-dichlorophenol 3, 5-dichlorocatechol 1, 2-dioxygenase gene gene location |
| url | https://www.academax.com/doi/10.3785/1008-9209.2001.03.0269 |
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