Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1
A 2,4-dichlorophenol degrading bacterium strain, designated as GT241-1, was isolated from the soil around drainage area of several factories which produced 2,4-dichlorophenol. It was identified as Pseudomonas sp. Strain GT241-1 can decompose 91% of 2, 4-dichlorophenol with original concentration of...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2001-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2001.03.0269 |
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| Summary: | A 2,4-dichlorophenol degrading bacterium strain, designated as GT241-1, was isolated from the soil around drainage area of several factories which produced 2,4-dichlorophenol. It was identified as Pseudomonas sp. Strain GT241-1 can decompose 91% of 2, 4-dichlorophenol with original concentration of 90 mg/L within 48 hours. GC/MS analysis revealed that the strain can degrade 2,4-dichlorophenol completely, without accumulation of intermediate metabolite. Heat treatment at 38℃ made 1.9% of GT241-1 strains lose its 2,4-dichlorophenol degrading ability. Strain GT241-1 has two big plasmids. Southern blot results showed that the 3, 5-dichlorocatechol 1,2-dioxygenase gene of strain GT241-1 locates on its about 11kb EcoRI/XbaI fragment. |
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| ISSN: | 1008-9209 2097-5155 |