Introduction of Salicornia europaea L into in vitro culture
The goal of the research was to develop a technology for introducing Salicornia europaea L.into in vitro culture. Methods of cultivating meristems and callus cultures were studied. To cultivate meristems, the tip of the apical bud were used as an explant. Fragments of stems and leaves were used toob...
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| Format: | Article |
| Language: | Russian |
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Maykop State Technological University
2024-04-01
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| Series: | Новые технологии |
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| Online Access: | https://newtechology.mkgtu.ru/jour/article/view/741 |
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| author | M. F. Koryazhkina N. A. Dmitrieva Е. V. Trizno A. B. Sediki A. M. Utesheva E. S. Skorobogatova |
| author_facet | M. F. Koryazhkina N. A. Dmitrieva Е. V. Trizno A. B. Sediki A. M. Utesheva E. S. Skorobogatova |
| author_sort | M. F. Koryazhkina |
| collection | DOAJ |
| description | The goal of the research was to develop a technology for introducing Salicornia europaea L.into in vitro culture. Methods of cultivating meristems and callus cultures were studied. To cultivate meristems, the tip of the apical bud were used as an explant. Fragments of stems and leaves were used toobtain callus tissue. To study the influence of various factors on germination, seeds were soaked in sterile tap water and in solutions of gibberellin, cytokinin, auxin and NaCl, and were also subjected to cold stratification (independently and with subsequent placement in Knop’s agarized medium). Salicornia seeds were sterilized with various antiseptics: 70% alcohol, 10% aqueous solution of sodium hypochlorite («Belizna»), amoxicillinand 3% hydrogen peroxide. The ability of the culture to form callus was studied in MS medium. As a result, it was determined that the highest germination of seeds was observed in Knop medium after treating theseeds with a suspension of green algae of the Scenedesmus genus, as well as after preliminary cold stratification, and slightly less after treating the seeds with a solution of NaCl and gibberellic acid. The most effective method of seed sterilization turned out to be treatment with alcohol followed by treatment with sodium hypochlorite. A comparative analysis of seed germination in filter paper in Petri dishes, Knop agar medium, Murashige and Skoog (hormone-free) was carried out. The ability of S. europaea L. to form callusin MS medium with phytohormones was assessed. Conclusion. To improve germination, it is recommended to subject the seeds to cold stratification. To obtain aseptic explants quickly, it is recommended to germinate the seeds in the Knop nutrient medium, having previously sterilized them with alcohol, then with sodiumhypochlorite, followed by washing with distilled water. The most suitable type of microclonal propagationfor S. europaea L. is the production of callus tissue followed by induction of organogenesis or embryogenesis. |
| format | Article |
| id | doaj-art-1ef965ae981443f0b40fdb7bb166acfe |
| institution | DOAJ |
| issn | 2072-0920 2713-0029 |
| language | Russian |
| publishDate | 2024-04-01 |
| publisher | Maykop State Technological University |
| record_format | Article |
| series | Новые технологии |
| spelling | doaj-art-1ef965ae981443f0b40fdb7bb166acfe2025-08-20T02:49:18ZrusMaykop State Technological UniversityНовые технологии2072-09202713-00292024-04-0120114615610.47370/2072-0920-2024-20-1-146-156666Introduction of Salicornia europaea L into in vitro cultureM. F. Koryazhkina0N. A. Dmitrieva1Е. V. Trizno2A. B. Sediki3A. M. Utesheva4E. S. Skorobogatova5State Autonomous Educational Institution of Additional Education of the Astrakhan Region «Regional School Technopark»«Salicornia Nutrition» LLCFederal State Budgetary Educational Institution of Higher Education SBEI HE «Astrakhan State Medical University» of the Ministry of Health of the Russian FederationState Autonomous Educational Institution of Additional Education of the Astrakhan Region «Regional School Technopark»State Autonomous Educational Institution of Additional Education of the Astrakhan Region «Regional School Technopark»State Autonomous Educational Institution of Additional Education of the Astrakhan Region «Regional School Technopark»The goal of the research was to develop a technology for introducing Salicornia europaea L.into in vitro culture. Methods of cultivating meristems and callus cultures were studied. To cultivate meristems, the tip of the apical bud were used as an explant. Fragments of stems and leaves were used toobtain callus tissue. To study the influence of various factors on germination, seeds were soaked in sterile tap water and in solutions of gibberellin, cytokinin, auxin and NaCl, and were also subjected to cold stratification (independently and with subsequent placement in Knop’s agarized medium). Salicornia seeds were sterilized with various antiseptics: 70% alcohol, 10% aqueous solution of sodium hypochlorite («Belizna»), amoxicillinand 3% hydrogen peroxide. The ability of the culture to form callus was studied in MS medium. As a result, it was determined that the highest germination of seeds was observed in Knop medium after treating theseeds with a suspension of green algae of the Scenedesmus genus, as well as after preliminary cold stratification, and slightly less after treating the seeds with a solution of NaCl and gibberellic acid. The most effective method of seed sterilization turned out to be treatment with alcohol followed by treatment with sodium hypochlorite. A comparative analysis of seed germination in filter paper in Petri dishes, Knop agar medium, Murashige and Skoog (hormone-free) was carried out. The ability of S. europaea L. to form callusin MS medium with phytohormones was assessed. Conclusion. To improve germination, it is recommended to subject the seeds to cold stratification. To obtain aseptic explants quickly, it is recommended to germinate the seeds in the Knop nutrient medium, having previously sterilized them with alcohol, then with sodiumhypochlorite, followed by washing with distilled water. The most suitable type of microclonal propagationfor S. europaea L. is the production of callus tissue followed by induction of organogenesis or embryogenesis.https://newtechology.mkgtu.ru/jour/article/view/741salicorniahalophytesalt tolerancegerminationgibberellic acidcold stratificationmicroclonal propagationin vitro culturecallus culturephytohormonesmurashige and skoogexplant |
| spellingShingle | M. F. Koryazhkina N. A. Dmitrieva Е. V. Trizno A. B. Sediki A. M. Utesheva E. S. Skorobogatova Introduction of Salicornia europaea L into in vitro culture Новые технологии salicornia halophyte salt tolerance germination gibberellic acid cold stratification microclonal propagation in vitro culture callus culture phytohormones murashige and skoog explant |
| title | Introduction of Salicornia europaea L into in vitro culture |
| title_full | Introduction of Salicornia europaea L into in vitro culture |
| title_fullStr | Introduction of Salicornia europaea L into in vitro culture |
| title_full_unstemmed | Introduction of Salicornia europaea L into in vitro culture |
| title_short | Introduction of Salicornia europaea L into in vitro culture |
| title_sort | introduction of salicornia europaea l into in vitro culture |
| topic | salicornia halophyte salt tolerance germination gibberellic acid cold stratification microclonal propagation in vitro culture callus culture phytohormones murashige and skoog explant |
| url | https://newtechology.mkgtu.ru/jour/article/view/741 |
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