Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes.

The innate immune system critically shapes diabetogenic adaptive immunity during type 1 diabetes (T1D) pathogenesis. While the role of tissue-infiltrating monocyte-derived macrophages in T1D is well established, the role of their tissue-resident counterparts remains undefined. We now demonstrate tha...

Full description

Saved in:
Bibliographic Details
Main Authors: Thomas B Thornley, Krishna A Agarwal, Periklis Kyriazis, Lingzhi Ma, Vaja Chipashvili, Jonathan E Aker, Sarantis Korniotis, Eva Csizmadia, Terry B Strom, Maria Koulmanda
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150792&type=printable
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850189037382402048
author Thomas B Thornley
Krishna A Agarwal
Periklis Kyriazis
Lingzhi Ma
Vaja Chipashvili
Jonathan E Aker
Sarantis Korniotis
Eva Csizmadia
Terry B Strom
Maria Koulmanda
author_facet Thomas B Thornley
Krishna A Agarwal
Periklis Kyriazis
Lingzhi Ma
Vaja Chipashvili
Jonathan E Aker
Sarantis Korniotis
Eva Csizmadia
Terry B Strom
Maria Koulmanda
author_sort Thomas B Thornley
collection DOAJ
description The innate immune system critically shapes diabetogenic adaptive immunity during type 1 diabetes (T1D) pathogenesis. While the role of tissue-infiltrating monocyte-derived macrophages in T1D is well established, the role of their tissue-resident counterparts remains undefined. We now demonstrate that islet resident macrophages (IRMs) from non-autoimmune mice have an immunoregulatory phenotype and powerfully induce FoxP3+ Tregs in vitro. The immunoregulatory phenotype and function of IRMs is compromised by TLR4 activation in vitro. Moreover, as T1D approaches in NOD mice, the immunoregulatory phenotype of IRMs is diminished as is their relative abundance compared to immunostimulatory DCs. Our findings suggest that maintenance of IRM abundance and their immunoregulatory phenotype may constitute a novel therapeutic strategy to prevent and/or cure T1D.
format Article
id doaj-art-1ee04bde033b4a88bda65b5fa8719471
institution OA Journals
issn 1932-6203
language English
publishDate 2016-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj-art-1ee04bde033b4a88bda65b5fa87194712025-08-20T02:15:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015079210.1371/journal.pone.0150792Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes.Thomas B ThornleyKrishna A AgarwalPeriklis KyriazisLingzhi MaVaja ChipashviliJonathan E AkerSarantis KorniotisEva CsizmadiaTerry B StromMaria KoulmandaThe innate immune system critically shapes diabetogenic adaptive immunity during type 1 diabetes (T1D) pathogenesis. While the role of tissue-infiltrating monocyte-derived macrophages in T1D is well established, the role of their tissue-resident counterparts remains undefined. We now demonstrate that islet resident macrophages (IRMs) from non-autoimmune mice have an immunoregulatory phenotype and powerfully induce FoxP3+ Tregs in vitro. The immunoregulatory phenotype and function of IRMs is compromised by TLR4 activation in vitro. Moreover, as T1D approaches in NOD mice, the immunoregulatory phenotype of IRMs is diminished as is their relative abundance compared to immunostimulatory DCs. Our findings suggest that maintenance of IRM abundance and their immunoregulatory phenotype may constitute a novel therapeutic strategy to prevent and/or cure T1D.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150792&type=printable
spellingShingle Thomas B Thornley
Krishna A Agarwal
Periklis Kyriazis
Lingzhi Ma
Vaja Chipashvili
Jonathan E Aker
Sarantis Korniotis
Eva Csizmadia
Terry B Strom
Maria Koulmanda
Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes.
PLoS ONE
title Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes.
title_full Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes.
title_fullStr Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes.
title_full_unstemmed Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes.
title_short Contrasting Roles of Islet Resident Immunoregulatory Macrophages and Dendritic Cells in Experimental Autoimmune Type 1 Diabetes.
title_sort contrasting roles of islet resident immunoregulatory macrophages and dendritic cells in experimental autoimmune type 1 diabetes
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150792&type=printable
work_keys_str_mv AT thomasbthornley contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes
AT krishnaaagarwal contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes
AT perikliskyriazis contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes
AT lingzhima contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes
AT vajachipashvili contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes
AT jonathaneaker contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes
AT sarantiskorniotis contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes
AT evacsizmadia contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes
AT terrybstrom contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes
AT mariakoulmanda contrastingrolesofisletresidentimmunoregulatorymacrophagesanddendriticcellsinexperimentalautoimmunetype1diabetes