Impact of Viruses on Prokaryotic Communities and Greenhouse Gas Emissions in Agricultural Soils

Abstract Viruses are abundant and ubiquitous in soil, but their importance in modulating greenhouse gas (GHG) emissions in terrestrial ecosystems remains largely unknown. Here, various loads of viral communities are introduced into paddy soils with different fertilization histories via a reciprocal...

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Main Authors: Xing Huang, Lucas P. P. Braga, Chenxiao Ding, Bokai Yang, Tida Ge, Hongjie Di, Yan He, Jianming Xu, Laurent Philippot, Yong Li
Format: Article
Language:English
Published: Wiley 2024-12-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202407223
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author Xing Huang
Lucas P. P. Braga
Chenxiao Ding
Bokai Yang
Tida Ge
Hongjie Di
Yan He
Jianming Xu
Laurent Philippot
Yong Li
author_facet Xing Huang
Lucas P. P. Braga
Chenxiao Ding
Bokai Yang
Tida Ge
Hongjie Di
Yan He
Jianming Xu
Laurent Philippot
Yong Li
author_sort Xing Huang
collection DOAJ
description Abstract Viruses are abundant and ubiquitous in soil, but their importance in modulating greenhouse gas (GHG) emissions in terrestrial ecosystems remains largely unknown. Here, various loads of viral communities are introduced into paddy soils with different fertilization histories via a reciprocal transplant approach to study the role of viruses in regulating greenhouse gas emissions and prokaryotic communities. The results showed that the addition of viruses has a strong impact on methane (CH4) and nitrous oxide (N2O) emissions and, to a minor extent, carbon dioxide (CO2) emissions, along with dissolved carbon and nitrogen pools, depending on soil fertilization history. The addition of a high viral load resulted in a decrease in microbial biomass carbon (MBC) by 31.4%, with changes in the relative abundance of 16.6% of dominant amplicon sequence variants (ASVs) in comparison to control treatments. More specifically, large effects of viral pressure are observed on some specific microbial communities with decreased relative abundance of prokaryotes that dissimilate sulfur compounds and increased relative abundance of Nanoarchaea. Structural equation modeling further highlighted the differential direct and indirect effects of viruses on CO2, N2O, and CH4 emissions. These findings underpin the understanding of the complex microbe‐virus interactions and advance current knowledge on soil virus ecology.
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spelling doaj-art-1ed81c539f45409a957c19024fcee2cc2025-08-20T02:55:53ZengWileyAdvanced Science2198-38442024-12-011148n/an/a10.1002/advs.202407223Impact of Viruses on Prokaryotic Communities and Greenhouse Gas Emissions in Agricultural SoilsXing Huang0Lucas P. P. Braga1Chenxiao Ding2Bokai Yang3Tida Ge4Hongjie Di5Yan He6Jianming Xu7Laurent Philippot8Yong Li9Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 ChinaDepartment of Plant Sciences University of Cambridge Cambridge CB2 3EA UKZhejiang Provincial Key Laboratory of Agricultural Resources and Environment College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 ChinaZhejiang Provincial Key Laboratory of Agricultural Resources and Environment College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‐products Institute of Plant Virology Ningbo University Ningbo 315211 ChinaZhejiang Provincial Key Laboratory of Agricultural Resources and Environment College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 ChinaZhejiang Provincial Key Laboratory of Agricultural Resources and Environment College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 ChinaZhejiang Provincial Key Laboratory of Agricultural Resources and Environment College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 ChinaUniversité Bourgogne INRAE Institut Agro Dijon Agroécologie Dijon 21000 FranceZhejiang Provincial Key Laboratory of Agricultural Resources and Environment College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 ChinaAbstract Viruses are abundant and ubiquitous in soil, but their importance in modulating greenhouse gas (GHG) emissions in terrestrial ecosystems remains largely unknown. Here, various loads of viral communities are introduced into paddy soils with different fertilization histories via a reciprocal transplant approach to study the role of viruses in regulating greenhouse gas emissions and prokaryotic communities. The results showed that the addition of viruses has a strong impact on methane (CH4) and nitrous oxide (N2O) emissions and, to a minor extent, carbon dioxide (CO2) emissions, along with dissolved carbon and nitrogen pools, depending on soil fertilization history. The addition of a high viral load resulted in a decrease in microbial biomass carbon (MBC) by 31.4%, with changes in the relative abundance of 16.6% of dominant amplicon sequence variants (ASVs) in comparison to control treatments. More specifically, large effects of viral pressure are observed on some specific microbial communities with decreased relative abundance of prokaryotes that dissimilate sulfur compounds and increased relative abundance of Nanoarchaea. Structural equation modeling further highlighted the differential direct and indirect effects of viruses on CO2, N2O, and CH4 emissions. These findings underpin the understanding of the complex microbe‐virus interactions and advance current knowledge on soil virus ecology.https://doi.org/10.1002/advs.202407223agricultural soilbiogeochemical cyclesgreenhouse gas emissionmicrobial functional traitsoil virusesviral shunt
spellingShingle Xing Huang
Lucas P. P. Braga
Chenxiao Ding
Bokai Yang
Tida Ge
Hongjie Di
Yan He
Jianming Xu
Laurent Philippot
Yong Li
Impact of Viruses on Prokaryotic Communities and Greenhouse Gas Emissions in Agricultural Soils
Advanced Science
agricultural soil
biogeochemical cycles
greenhouse gas emission
microbial functional trait
soil viruses
viral shunt
title Impact of Viruses on Prokaryotic Communities and Greenhouse Gas Emissions in Agricultural Soils
title_full Impact of Viruses on Prokaryotic Communities and Greenhouse Gas Emissions in Agricultural Soils
title_fullStr Impact of Viruses on Prokaryotic Communities and Greenhouse Gas Emissions in Agricultural Soils
title_full_unstemmed Impact of Viruses on Prokaryotic Communities and Greenhouse Gas Emissions in Agricultural Soils
title_short Impact of Viruses on Prokaryotic Communities and Greenhouse Gas Emissions in Agricultural Soils
title_sort impact of viruses on prokaryotic communities and greenhouse gas emissions in agricultural soils
topic agricultural soil
biogeochemical cycles
greenhouse gas emission
microbial functional trait
soil viruses
viral shunt
url https://doi.org/10.1002/advs.202407223
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