Characterization of Diabetic Neuropathy in the Zucker Diabetic Sprague-Dawley Rat: A New Animal Model for Type 2 Diabetes
Recently a new rat model for type 2 diabetes the Zucker diabetic Sprague-Dawley (ZDSD/Pco) was created. In this study we sought to characterize the development of diabetic neuropathy in ZDSD rats using age-matched Sprague-Dawley rats as a control. Rats were examined at 34 weeks of age 12 weeks after...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2014/714273 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849306849867726848 |
|---|---|
| author | Eric P. Davidson Lawrence J. Coppey Amey Holmes Sergey Lupachyk Brian L. Dake Christine L. Oltman Richard G. Peterson Mark A. Yorek |
| author_facet | Eric P. Davidson Lawrence J. Coppey Amey Holmes Sergey Lupachyk Brian L. Dake Christine L. Oltman Richard G. Peterson Mark A. Yorek |
| author_sort | Eric P. Davidson |
| collection | DOAJ |
| description | Recently a new rat model for type 2 diabetes the Zucker diabetic Sprague-Dawley (ZDSD/Pco) was created. In this study we sought to characterize the development of diabetic neuropathy in ZDSD rats using age-matched Sprague-Dawley rats as a control. Rats were examined at 34 weeks of age 12 weeks after the onset of hyperglycemia in ZDSD rats. At this time ZDSD rats were severely insulin resistant with slowing of both motor and sensory nerve conduction velocities. ZDSD rats also had fatty livers, elevated serum free fatty acids, triglycerides, and cholesterol, and elevated sciatic nerve nitrotyrosine levels. The corneas of ZDSD rats exhibited a decrease in subbasal epithelial corneal nerves and sensitivity. ZDSD rats were hypoalgesic but intraepidermal nerve fibers in the skin of the hindpaw were normal compared to Sprague-Dawley rats. However, the number of Langerhans cells was decreased. Vascular reactivity of epineurial arterioles, blood vessels that provide circulation to the sciatic nerve, to acetylcholine and calcitonin gene-related peptide was impaired in ZDSD rats. These data indicate that ZDSD rats develop many of the neural complications associated with type 2 diabetes and are a good animal model for preclinical investigations of drug development for diabetic neuropathy. |
| format | Article |
| id | doaj-art-1ecf2629e59b4ff0af1f668cc1bc67fe |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-1ecf2629e59b4ff0af1f668cc1bc67fe2025-08-20T03:54:57ZengWileyJournal of Diabetes Research2314-67452314-67532014-01-01201410.1155/2014/714273714273Characterization of Diabetic Neuropathy in the Zucker Diabetic Sprague-Dawley Rat: A New Animal Model for Type 2 DiabetesEric P. Davidson0Lawrence J. Coppey1Amey Holmes2Sergey Lupachyk3Brian L. Dake4Christine L. Oltman5Richard G. Peterson6Mark A. Yorek7Department of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USADepartment of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USADepartment of Veterans Affairs Iowa City Health Care System, Iowa City, IA 52246, USADepartment of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USADepartment of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USADepartment of Veterans Affairs Iowa City Health Care System, Iowa City, IA 52246, USAPreClinOmics Inc., Indianapolis, IN 46268, USADepartment of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USARecently a new rat model for type 2 diabetes the Zucker diabetic Sprague-Dawley (ZDSD/Pco) was created. In this study we sought to characterize the development of diabetic neuropathy in ZDSD rats using age-matched Sprague-Dawley rats as a control. Rats were examined at 34 weeks of age 12 weeks after the onset of hyperglycemia in ZDSD rats. At this time ZDSD rats were severely insulin resistant with slowing of both motor and sensory nerve conduction velocities. ZDSD rats also had fatty livers, elevated serum free fatty acids, triglycerides, and cholesterol, and elevated sciatic nerve nitrotyrosine levels. The corneas of ZDSD rats exhibited a decrease in subbasal epithelial corneal nerves and sensitivity. ZDSD rats were hypoalgesic but intraepidermal nerve fibers in the skin of the hindpaw were normal compared to Sprague-Dawley rats. However, the number of Langerhans cells was decreased. Vascular reactivity of epineurial arterioles, blood vessels that provide circulation to the sciatic nerve, to acetylcholine and calcitonin gene-related peptide was impaired in ZDSD rats. These data indicate that ZDSD rats develop many of the neural complications associated with type 2 diabetes and are a good animal model for preclinical investigations of drug development for diabetic neuropathy.http://dx.doi.org/10.1155/2014/714273 |
| spellingShingle | Eric P. Davidson Lawrence J. Coppey Amey Holmes Sergey Lupachyk Brian L. Dake Christine L. Oltman Richard G. Peterson Mark A. Yorek Characterization of Diabetic Neuropathy in the Zucker Diabetic Sprague-Dawley Rat: A New Animal Model for Type 2 Diabetes Journal of Diabetes Research |
| title | Characterization of Diabetic Neuropathy in the Zucker Diabetic Sprague-Dawley Rat: A New Animal Model for Type 2 Diabetes |
| title_full | Characterization of Diabetic Neuropathy in the Zucker Diabetic Sprague-Dawley Rat: A New Animal Model for Type 2 Diabetes |
| title_fullStr | Characterization of Diabetic Neuropathy in the Zucker Diabetic Sprague-Dawley Rat: A New Animal Model for Type 2 Diabetes |
| title_full_unstemmed | Characterization of Diabetic Neuropathy in the Zucker Diabetic Sprague-Dawley Rat: A New Animal Model for Type 2 Diabetes |
| title_short | Characterization of Diabetic Neuropathy in the Zucker Diabetic Sprague-Dawley Rat: A New Animal Model for Type 2 Diabetes |
| title_sort | characterization of diabetic neuropathy in the zucker diabetic sprague dawley rat a new animal model for type 2 diabetes |
| url | http://dx.doi.org/10.1155/2014/714273 |
| work_keys_str_mv | AT ericpdavidson characterizationofdiabeticneuropathyinthezuckerdiabeticspraguedawleyratanewanimalmodelfortype2diabetes AT lawrencejcoppey characterizationofdiabeticneuropathyinthezuckerdiabeticspraguedawleyratanewanimalmodelfortype2diabetes AT ameyholmes characterizationofdiabeticneuropathyinthezuckerdiabeticspraguedawleyratanewanimalmodelfortype2diabetes AT sergeylupachyk characterizationofdiabeticneuropathyinthezuckerdiabeticspraguedawleyratanewanimalmodelfortype2diabetes AT brianldake characterizationofdiabeticneuropathyinthezuckerdiabeticspraguedawleyratanewanimalmodelfortype2diabetes AT christineloltman characterizationofdiabeticneuropathyinthezuckerdiabeticspraguedawleyratanewanimalmodelfortype2diabetes AT richardgpeterson characterizationofdiabeticneuropathyinthezuckerdiabeticspraguedawleyratanewanimalmodelfortype2diabetes AT markayorek characterizationofdiabeticneuropathyinthezuckerdiabeticspraguedawleyratanewanimalmodelfortype2diabetes |