Bacterial Cellulose Membrane Experimentally Implanted in the Peritoneum of Wistar Rats—Inflammatory Immunoreactivity and Oxidative Stress
Bacterial cellulose (BC) has been used for various applications; however, studies investigating the immunohistochemical characteristics of the inflammatory and scarring component in BC implanted in the peritoneum in vivo have not yet been fully described. This study aimed to evaluate the systemic an...
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2024-10-01
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| author | Karina Oliveira Santos Rebecca Bertolo Natasha Lien de Almeida Ibanez Mônica Rodrigues Alves Tatiana Pessoa Onuma Gabriella Costa Ribeiro Anna Julia de Souza Porto Cláudio Gustavo Barbeito Luciana Pinato Angela Faustino Jozala Denise Grotto Alessandre Hataka |
| author_facet | Karina Oliveira Santos Rebecca Bertolo Natasha Lien de Almeida Ibanez Mônica Rodrigues Alves Tatiana Pessoa Onuma Gabriella Costa Ribeiro Anna Julia de Souza Porto Cláudio Gustavo Barbeito Luciana Pinato Angela Faustino Jozala Denise Grotto Alessandre Hataka |
| author_sort | Karina Oliveira Santos |
| collection | DOAJ |
| description | Bacterial cellulose (BC) has been used for various applications; however, studies investigating the immunohistochemical characteristics of the inflammatory and scarring component in BC implanted in the peritoneum in vivo have not yet been fully described. This study aimed to evaluate the systemic and organic safety of BC through oxidative stress, blood, and serum biochemical markers, as well as the late inflammatory response in rats, using histopathology and immunohistochemistry. Forty-three rats (26 males; 17 females) received BC in the peritoneal cavity (implanted group—IG), while twenty-seven rats (12 males; 15 females) served as the control (sham group—SG). Sixty days after surgery, oxidative stress in tissues, blood biochemical markers, and histopathological and immunohistochemical analyses for lymphocytes, macrophages, collagen, and vascular response around the BC were assessed. Only one oxidative stress marker, glutathione peroxidase, was elevated in the liver of IG rats. Creatine kinase MB and lactate dehydrogenase levels were significantly lower in IG animals. Histopathological analysis showed granulomatous inflammation in 93% of IG rats, with 74% of mild intensity. Immunohistochemistry revealed a significant macrophage presence (F4/80), with CD3, CD20, and F4/80 markers indicating differences favoring macrophages. In conclusion, BC implantation in the peritoneum induces a foreign body granulomatous response with prominent macrophage presence (F4/80). Type I and III collagen were observed around the membrane, and vascularization was intense 60 days post-implantation. From a biochemical and oxidative stress perspective, BC seems to be a safe material to be used in the peritoneal cavity. |
| format | Article |
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| institution | OA Journals |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-1ec8655a5fe14015a67800bec6edba112025-08-20T02:08:03ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-10-014611117291174810.3390/cimb46110697Bacterial Cellulose Membrane Experimentally Implanted in the Peritoneum of Wistar Rats—Inflammatory Immunoreactivity and Oxidative StressKarina Oliveira Santos0Rebecca Bertolo1Natasha Lien de Almeida Ibanez2Mônica Rodrigues Alves3Tatiana Pessoa Onuma4Gabriella Costa Ribeiro5Anna Julia de Souza Porto6Cláudio Gustavo Barbeito7Luciana Pinato8Angela Faustino Jozala9Denise Grotto10Alessandre Hataka11Department of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18610-307, SP, BrazilDepartment of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18610-307, SP, BrazilDepartment of Pharmacy, University of Sorocaba, Sorocaba 18023-000, SP, BrazilDepartment of Pharmacy, University of Sorocaba, Sorocaba 18023-000, SP, BrazilDepartment of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18610-307, SP, BrazilDepartment of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18610-307, SP, BrazilDepartment of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18610-307, SP, BrazilLaboratory of Descriptive, Experimental and Comparative Histology and Embryology, School of Veterinary Sciences, National University of La Plata, National Scientific and Technical Research Council, La Plata 1900, CP, ArgentinaDepartment of Speech, Language and Hearing Sciences, São Paulo State University (UNESP), Marilia 17525-900, SP, BrazilDepartment of Pharmacy, University of Sorocaba, Sorocaba 18023-000, SP, BrazilDepartment of Pharmacy, University of Sorocaba, Sorocaba 18023-000, SP, BrazilDepartment of Veterinary Clinical Sciences, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18610-307, SP, BrazilBacterial cellulose (BC) has been used for various applications; however, studies investigating the immunohistochemical characteristics of the inflammatory and scarring component in BC implanted in the peritoneum in vivo have not yet been fully described. This study aimed to evaluate the systemic and organic safety of BC through oxidative stress, blood, and serum biochemical markers, as well as the late inflammatory response in rats, using histopathology and immunohistochemistry. Forty-three rats (26 males; 17 females) received BC in the peritoneal cavity (implanted group—IG), while twenty-seven rats (12 males; 15 females) served as the control (sham group—SG). Sixty days after surgery, oxidative stress in tissues, blood biochemical markers, and histopathological and immunohistochemical analyses for lymphocytes, macrophages, collagen, and vascular response around the BC were assessed. Only one oxidative stress marker, glutathione peroxidase, was elevated in the liver of IG rats. Creatine kinase MB and lactate dehydrogenase levels were significantly lower in IG animals. Histopathological analysis showed granulomatous inflammation in 93% of IG rats, with 74% of mild intensity. Immunohistochemistry revealed a significant macrophage presence (F4/80), with CD3, CD20, and F4/80 markers indicating differences favoring macrophages. In conclusion, BC implantation in the peritoneum induces a foreign body granulomatous response with prominent macrophage presence (F4/80). Type I and III collagen were observed around the membrane, and vascularization was intense 60 days post-implantation. From a biochemical and oxidative stress perspective, BC seems to be a safe material to be used in the peritoneal cavity.https://www.mdpi.com/1467-3045/46/11/697bacterial celluloseperitonealimmunohistochemistryoxidative stressrat |
| spellingShingle | Karina Oliveira Santos Rebecca Bertolo Natasha Lien de Almeida Ibanez Mônica Rodrigues Alves Tatiana Pessoa Onuma Gabriella Costa Ribeiro Anna Julia de Souza Porto Cláudio Gustavo Barbeito Luciana Pinato Angela Faustino Jozala Denise Grotto Alessandre Hataka Bacterial Cellulose Membrane Experimentally Implanted in the Peritoneum of Wistar Rats—Inflammatory Immunoreactivity and Oxidative Stress Current Issues in Molecular Biology bacterial cellulose peritoneal immunohistochemistry oxidative stress rat |
| title | Bacterial Cellulose Membrane Experimentally Implanted in the Peritoneum of Wistar Rats—Inflammatory Immunoreactivity and Oxidative Stress |
| title_full | Bacterial Cellulose Membrane Experimentally Implanted in the Peritoneum of Wistar Rats—Inflammatory Immunoreactivity and Oxidative Stress |
| title_fullStr | Bacterial Cellulose Membrane Experimentally Implanted in the Peritoneum of Wistar Rats—Inflammatory Immunoreactivity and Oxidative Stress |
| title_full_unstemmed | Bacterial Cellulose Membrane Experimentally Implanted in the Peritoneum of Wistar Rats—Inflammatory Immunoreactivity and Oxidative Stress |
| title_short | Bacterial Cellulose Membrane Experimentally Implanted in the Peritoneum of Wistar Rats—Inflammatory Immunoreactivity and Oxidative Stress |
| title_sort | bacterial cellulose membrane experimentally implanted in the peritoneum of wistar rats inflammatory immunoreactivity and oxidative stress |
| topic | bacterial cellulose peritoneal immunohistochemistry oxidative stress rat |
| url | https://www.mdpi.com/1467-3045/46/11/697 |
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