Expression Profiling and Functional Analysis of Candidate Odorant Receptors in <i>Galeruca daurica</i>
<i>Galeruca daurica</i> (Joannis) is an oligophagous pest in the grasslands of Inner Mongolia, China, which feed mainly on <i>Allium</i> spp. Odorant receptors (ORs) play an important role in the olfactory system in insects, and function together with olfactory co-receptor (O...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
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| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/13/7/563 |
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| Summary: | <i>Galeruca daurica</i> (Joannis) is an oligophagous pest in the grasslands of Inner Mongolia, China, which feed mainly on <i>Allium</i> spp. Odorant receptors (ORs) play an important role in the olfactory system in insects, and function together with olfactory co-receptor (ORco). In this study, 21 OR genes were identified from the transcriptome database of <i>G. daurica</i> adults, and named <i>GdauOR1-20</i> and <i>GdauORco</i>. The expression profiles were examined by RT-qPCR and RNA interference (RNAi) and electroantennogram (EAG) experiments were conducted to further identify the olfactory functions of <i>GdauOR4</i>, <i>GdauOR11</i>, <i>GdauOR15</i>, and <i>GdauORco</i>. It was found that 15 <i>GdauORs</i> (<i>OR1</i>, <i>OR3-6</i>, <i>OR8</i>, <i>OR11-13</i>, <i>OR15</i>, <i>OR17-20</i>, and <i>ORco</i>) were mainly expressed in antennae, and the expression levels of <i>GdauORs</i> in adults were affected by age. When <i>GdauOR4</i>, <i>GdauOR15</i>, and <i>GdauORco</i> were silenced by RNAi, the electrophysiological responses to host plant volatiles were significantly decreased in <i>G. daurica</i>. This study lays a necessary foundation for clarifying the mechanism on finding host plants in <i>G. daurica</i>. |
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| ISSN: | 2075-4450 |