Design and implementation of a lab-on-a-chip for assisted reproductive technologies
Introduction: The microfluidic device is highly optimized to remove oocytes from the cumulus-corona cell mass surrounding them. Additionally, it effectively captures and immobilizes the oocytes, aiding in assessing their quality and facilitating the injection of sperm into the oocyte. In this study,...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2024-07-01
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| Series: | BioImpacts |
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| Online Access: | https://bi.tbzmed.ac.ir/PDF/bi-14-28902.pdf |
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| author | Firooz Safaefar Javad Karamdel Hadi Veladi Masoud Maleki |
| author_facet | Firooz Safaefar Javad Karamdel Hadi Veladi Masoud Maleki |
| author_sort | Firooz Safaefar |
| collection | DOAJ |
| description | Introduction: The microfluidic device is highly optimized to remove oocytes from the cumulus-corona cell mass surrounding them. Additionally, it effectively captures and immobilizes the oocytes, aiding in assessing their quality and facilitating the injection of sperm into the oocyte. In this study, a novel microfluidic chip was designed and manufactured using conventional soft lithography methods. Methods: This research proposes the utilization of a microfluidic chip as a substitute for the conventional manual procedures involved in oocyte denudation, trapping, and immobilization. The microfluidic chip was modeled and simulated using COMSOL Multiphysics® 5.2 software to optimize and enhance its design and performance. The microfluidic chip was fabricated using conventional injection molding techniques on a polydimethylsiloxane substrate by employing soft lithography methods. Results: A hydrostatic force was applied to guide the oocyte through predetermined pathways to eliminate the cumulus cells surrounding the oocyte. The oocyte was subsequently confined within the designated trap region by utilizing hydraulic resistance along the paths and immobilized by applying vacuum force. Conclusion: The application of this chip necessitates a lower level of operator expertise compared to enzymatic and mechanical techniques. Moreover, it is feasible to continuously monitor the oocyte's state throughout the procedure. There is a reduced need for cultural media compared to more standard approaches. |
| format | Article |
| id | doaj-art-1ebe4d311c46454eaada3a30533a5ee4 |
| institution | Kabale University |
| issn | 2228-5652 2228-5660 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | BioImpacts |
| spelling | doaj-art-1ebe4d311c46454eaada3a30533a5ee42025-01-18T09:59:44ZengTabriz University of Medical SciencesBioImpacts2228-56522228-56602024-07-01144289022890210.34172/bi.2023.28902bi-28902Design and implementation of a lab-on-a-chip for assisted reproductive technologiesFirooz Safaefar0Javad Karamdel1Hadi Veladi2Masoud Maleki3Department of Biomedical Engineering, Faculty of Technical and Engineering, South Tehran Branch, Islamic Azad University, Tehran, IranDepartment of Electrical Engineering, Faculty of Technical and Engineering, South Tehran Branch, Islamic Azad University, Tehran, IranDepartment of Biomedical Engineering, Faculty of Technical and Engineering, South Tehran Branch, Islamic Azad University, Tehran, IranDepartment of Biomedical Engineering, Faculty of Technical and Engineering, South Tehran Branch, Islamic Azad University, Tehran, IranIntroduction: The microfluidic device is highly optimized to remove oocytes from the cumulus-corona cell mass surrounding them. Additionally, it effectively captures and immobilizes the oocytes, aiding in assessing their quality and facilitating the injection of sperm into the oocyte. In this study, a novel microfluidic chip was designed and manufactured using conventional soft lithography methods. Methods: This research proposes the utilization of a microfluidic chip as a substitute for the conventional manual procedures involved in oocyte denudation, trapping, and immobilization. The microfluidic chip was modeled and simulated using COMSOL Multiphysics® 5.2 software to optimize and enhance its design and performance. The microfluidic chip was fabricated using conventional injection molding techniques on a polydimethylsiloxane substrate by employing soft lithography methods. Results: A hydrostatic force was applied to guide the oocyte through predetermined pathways to eliminate the cumulus cells surrounding the oocyte. The oocyte was subsequently confined within the designated trap region by utilizing hydraulic resistance along the paths and immobilized by applying vacuum force. Conclusion: The application of this chip necessitates a lower level of operator expertise compared to enzymatic and mechanical techniques. Moreover, it is feasible to continuously monitor the oocyte's state throughout the procedure. There is a reduced need for cultural media compared to more standard approaches.https://bi.tbzmed.ac.ir/PDF/bi-14-28902.pdfmicrofluidic chiplab-on-a-chipassisted reproductive technologycomsol multiphysics 5.2 |
| spellingShingle | Firooz Safaefar Javad Karamdel Hadi Veladi Masoud Maleki Design and implementation of a lab-on-a-chip for assisted reproductive technologies BioImpacts microfluidic chip lab-on-a-chip assisted reproductive technology comsol multiphysics 5.2 |
| title | Design and implementation of a lab-on-a-chip for assisted reproductive technologies |
| title_full | Design and implementation of a lab-on-a-chip for assisted reproductive technologies |
| title_fullStr | Design and implementation of a lab-on-a-chip for assisted reproductive technologies |
| title_full_unstemmed | Design and implementation of a lab-on-a-chip for assisted reproductive technologies |
| title_short | Design and implementation of a lab-on-a-chip for assisted reproductive technologies |
| title_sort | design and implementation of a lab on a chip for assisted reproductive technologies |
| topic | microfluidic chip lab-on-a-chip assisted reproductive technology comsol multiphysics 5.2 |
| url | https://bi.tbzmed.ac.ir/PDF/bi-14-28902.pdf |
| work_keys_str_mv | AT firoozsafaefar designandimplementationofalabonachipforassistedreproductivetechnologies AT javadkaramdel designandimplementationofalabonachipforassistedreproductivetechnologies AT hadiveladi designandimplementationofalabonachipforassistedreproductivetechnologies AT masoudmaleki designandimplementationofalabonachipforassistedreproductivetechnologies |