The clinical significance of intraoperative lavage fluid culture during pancreaticoduodenectomy on organ/space surgical site infection
Abstract Organ/space surgical site infection (SSI) are common after pancreaticoduodenectomy (PD). There is limited research on the clinical impact of intraoperative lavage fluid contamination in patients undergoing PD. One hundred five patients who underwent PD between August 2022 and July 2023 were...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-11-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-80245-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850163248791289856 |
|---|---|
| author | Yifei Yang Jianjie Sheng Chenglin Lu Hao Cheng Gang Li Liang Mao Chuang Chen Yudong Qiu Chang Liu Xu Fu |
| author_facet | Yifei Yang Jianjie Sheng Chenglin Lu Hao Cheng Gang Li Liang Mao Chuang Chen Yudong Qiu Chang Liu Xu Fu |
| author_sort | Yifei Yang |
| collection | DOAJ |
| description | Abstract Organ/space surgical site infection (SSI) are common after pancreaticoduodenectomy (PD). There is limited research on the clinical impact of intraoperative lavage fluid contamination in patients undergoing PD. One hundred five patients who underwent PD between August 2022 and July 2023 were retrospectively enrolled. The intraoperative bile and peritoneal lavage were collected for bacterial culture. Postoperative drainage bacterial cultures were performed every 2–3 days thereafter until drains were all removed. The bacteria isolated from intraoperative lavage fluid, intraoperative bile, and postoperative drainage fluid were examined in detail. The risk factors associated with positive intraoperative lavage fluid culture were analyzed through both univariate and multivariate analyses. Organ/space SSI occurred in 59(56.2%) of the 105 patients. The positivity rates of cultures in intraoperative lavage fluid, intraoperative bile, and postoperative drainage fluid were found to be 41.0%, 67.6%, and 84.8%, respectively. Patients with positive intraoperative lavage fluid culture had a significantly higher occurrence of organ/space SSI compared to the negative group (69.0% vs. 29.4%, P < 0.001). Preoperative biliary drainage (PBD) was identified as the only independent risk factor for the contamination of intraoperative lavage fluid (OR = 7.687, 95% CI: 2.164–27.300, P = 0.002). K. pneumoniae was the most common isolates both in the intraoperative lavage fluid and postoperative drainage fluid. Intraoperative lavage fluid contamination closely correlated with organ/space SSI after PD. Meanwhile, PBD was the only risk factor for the contamination of intraoperative lavage fluid. |
| format | Article |
| id | doaj-art-1ebe251c76fc4d24b01658ca456b5802 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-1ebe251c76fc4d24b01658ca456b58022025-08-20T02:22:20ZengNature PortfolioScientific Reports2045-23222024-11-011411810.1038/s41598-024-80245-0The clinical significance of intraoperative lavage fluid culture during pancreaticoduodenectomy on organ/space surgical site infectionYifei Yang0Jianjie Sheng1Chenglin Lu2Hao Cheng3Gang Li4Liang Mao5Chuang Chen6Yudong Qiu7Chang Liu8Xu Fu9Department of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese MedicineDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityDepartment of Hepatopancreatobiliary Surgery, The Affiliated Huai’ an Hospital of Xuzhou Medical UniversityDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing University of Chinese MedicineDepartment of Clinical Laboratory, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityDepartment of Pancreatic and Metabolic Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing UniversityAbstract Organ/space surgical site infection (SSI) are common after pancreaticoduodenectomy (PD). There is limited research on the clinical impact of intraoperative lavage fluid contamination in patients undergoing PD. One hundred five patients who underwent PD between August 2022 and July 2023 were retrospectively enrolled. The intraoperative bile and peritoneal lavage were collected for bacterial culture. Postoperative drainage bacterial cultures were performed every 2–3 days thereafter until drains were all removed. The bacteria isolated from intraoperative lavage fluid, intraoperative bile, and postoperative drainage fluid were examined in detail. The risk factors associated with positive intraoperative lavage fluid culture were analyzed through both univariate and multivariate analyses. Organ/space SSI occurred in 59(56.2%) of the 105 patients. The positivity rates of cultures in intraoperative lavage fluid, intraoperative bile, and postoperative drainage fluid were found to be 41.0%, 67.6%, and 84.8%, respectively. Patients with positive intraoperative lavage fluid culture had a significantly higher occurrence of organ/space SSI compared to the negative group (69.0% vs. 29.4%, P < 0.001). Preoperative biliary drainage (PBD) was identified as the only independent risk factor for the contamination of intraoperative lavage fluid (OR = 7.687, 95% CI: 2.164–27.300, P = 0.002). K. pneumoniae was the most common isolates both in the intraoperative lavage fluid and postoperative drainage fluid. Intraoperative lavage fluid contamination closely correlated with organ/space SSI after PD. Meanwhile, PBD was the only risk factor for the contamination of intraoperative lavage fluid.https://doi.org/10.1038/s41598-024-80245-0PancreaticoduodenectomySurgical site infectionIntraoperative lavage |
| spellingShingle | Yifei Yang Jianjie Sheng Chenglin Lu Hao Cheng Gang Li Liang Mao Chuang Chen Yudong Qiu Chang Liu Xu Fu The clinical significance of intraoperative lavage fluid culture during pancreaticoduodenectomy on organ/space surgical site infection Scientific Reports Pancreaticoduodenectomy Surgical site infection Intraoperative lavage |
| title | The clinical significance of intraoperative lavage fluid culture during pancreaticoduodenectomy on organ/space surgical site infection |
| title_full | The clinical significance of intraoperative lavage fluid culture during pancreaticoduodenectomy on organ/space surgical site infection |
| title_fullStr | The clinical significance of intraoperative lavage fluid culture during pancreaticoduodenectomy on organ/space surgical site infection |
| title_full_unstemmed | The clinical significance of intraoperative lavage fluid culture during pancreaticoduodenectomy on organ/space surgical site infection |
| title_short | The clinical significance of intraoperative lavage fluid culture during pancreaticoduodenectomy on organ/space surgical site infection |
| title_sort | clinical significance of intraoperative lavage fluid culture during pancreaticoduodenectomy on organ space surgical site infection |
| topic | Pancreaticoduodenectomy Surgical site infection Intraoperative lavage |
| url | https://doi.org/10.1038/s41598-024-80245-0 |
| work_keys_str_mv | AT yifeiyang theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT jianjiesheng theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT chenglinlu theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT haocheng theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT gangli theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT liangmao theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT chuangchen theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT yudongqiu theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT changliu theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT xufu theclinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT yifeiyang clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT jianjiesheng clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT chenglinlu clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT haocheng clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT gangli clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT liangmao clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT chuangchen clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT yudongqiu clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT changliu clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection AT xufu clinicalsignificanceofintraoperativelavagefluidcultureduringpancreaticoduodenectomyonorganspacesurgicalsiteinfection |