Evaluation of Cytokine Profile in Canine Malignant Oral Melanoma
Ten dogs with oral malignant melanoma were evaluated and treated with surgery, of which four dogs were diagnosed with melanotic melanoma and six were diagnosed with amelanotic melanoma. Serum samples from oral malignant melanoma (OMM) were collected at baseline, the day of the surgery, and every 3–4...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-06-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/12/7/627 |
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| author | Carmen G. Pérez-Santana Sara E. Cazorla-Rivero Ana A. Jiménez-Alonso Francisco Rodríguez-Esparragón Jesús María González Martín Ruth Henríquez-Cabrera Bernardino Clavo-Varas Enrique Rodríguez Grau-Bassas |
| author_facet | Carmen G. Pérez-Santana Sara E. Cazorla-Rivero Ana A. Jiménez-Alonso Francisco Rodríguez-Esparragón Jesús María González Martín Ruth Henríquez-Cabrera Bernardino Clavo-Varas Enrique Rodríguez Grau-Bassas |
| author_sort | Carmen G. Pérez-Santana |
| collection | DOAJ |
| description | Ten dogs with oral malignant melanoma were evaluated and treated with surgery, of which four dogs were diagnosed with melanotic melanoma and six were diagnosed with amelanotic melanoma. Serum samples from oral malignant melanoma (OMM) were collected at baseline, the day of the surgery, and every 3–4 months, during which time a clinical examination and chest X-rays were performed. Concentrations of GM-CSF, IFN-γ, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, IP-10, KC-like, MCP-1, and TNFα were quantified. Follow-up samples indicated that after the removal of malignant melanoma, the serum levels of GM-CSF, IFN-γ, MCP-1, IL-18, and IL-2 increased significantly. In contrast, when comparing samples from dogs with OMM to those of patients in remission, the concentrations of IL-7 and MCP-1 were significantly higher in the remission samples than in the OMM samples. Furthermore, when comparing the serum concentrations between the OMM-metastasis samples and those patients in remission, elevated levels of MCP-1 were associated with poorer overall survival due to the development of OMM metastasis. Finally, a comparison of cytokines in the melanotic OMM and amelanotic OMM samples revealed that the amelanotic OMM samples exhibited higher concentrations of IL-6, IL-10, and IL-15 compared to the melanotic OMM samples. |
| format | Article |
| id | doaj-art-1ebaad332dfe49c1a2f64ac9440d5499 |
| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-1ebaad332dfe49c1a2f64ac9440d54992025-08-20T03:56:49ZengMDPI AGVeterinary Sciences2306-73812025-06-0112762710.3390/vetsci12070627Evaluation of Cytokine Profile in Canine Malignant Oral MelanomaCarmen G. Pérez-Santana0Sara E. Cazorla-Rivero1Ana A. Jiménez-Alonso2Francisco Rodríguez-Esparragón3Jesús María González Martín4Ruth Henríquez-Cabrera5Bernardino Clavo-Varas6Enrique Rodríguez Grau-Bassas7Instituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA), Universidad de Las Palmas de Gran Canaria (ULPGC), 35400 Arucas, SpainUnidad de Investigación Hospital Universitario de Gran Canaria Dr. Negrín, 35010 Las Palmas de Gran Canaria, SpainInstituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA), Universidad de Las Palmas de Gran Canaria (ULPGC), 35400 Arucas, SpainUnidad de Investigación Hospital Universitario de Gran Canaria Dr. Negrín, 35010 Las Palmas de Gran Canaria, SpainUnidad de Investigación Hospital Universitario de Gran Canaria Dr. Negrín, 35010 Las Palmas de Gran Canaria, SpainUnidad de Investigación Hospital Universitario de Gran Canaria Dr. Negrín, 35010 Las Palmas de Gran Canaria, SpainUnidad de Investigación Hospital Universitario de Gran Canaria Dr. Negrín, 35010 Las Palmas de Gran Canaria, SpainInstituto Universitario de Sanidad Animal y Seguridad Alimentaria (IUSA), Universidad de Las Palmas de Gran Canaria (ULPGC), 35400 Arucas, SpainTen dogs with oral malignant melanoma were evaluated and treated with surgery, of which four dogs were diagnosed with melanotic melanoma and six were diagnosed with amelanotic melanoma. Serum samples from oral malignant melanoma (OMM) were collected at baseline, the day of the surgery, and every 3–4 months, during which time a clinical examination and chest X-rays were performed. Concentrations of GM-CSF, IFN-γ, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, IP-10, KC-like, MCP-1, and TNFα were quantified. Follow-up samples indicated that after the removal of malignant melanoma, the serum levels of GM-CSF, IFN-γ, MCP-1, IL-18, and IL-2 increased significantly. In contrast, when comparing samples from dogs with OMM to those of patients in remission, the concentrations of IL-7 and MCP-1 were significantly higher in the remission samples than in the OMM samples. Furthermore, when comparing the serum concentrations between the OMM-metastasis samples and those patients in remission, elevated levels of MCP-1 were associated with poorer overall survival due to the development of OMM metastasis. Finally, a comparison of cytokines in the melanotic OMM and amelanotic OMM samples revealed that the amelanotic OMM samples exhibited higher concentrations of IL-6, IL-10, and IL-15 compared to the melanotic OMM samples.https://www.mdpi.com/2306-7381/12/7/627cancerdogsoral melanomaoncologycytokineimmunotherapy |
| spellingShingle | Carmen G. Pérez-Santana Sara E. Cazorla-Rivero Ana A. Jiménez-Alonso Francisco Rodríguez-Esparragón Jesús María González Martín Ruth Henríquez-Cabrera Bernardino Clavo-Varas Enrique Rodríguez Grau-Bassas Evaluation of Cytokine Profile in Canine Malignant Oral Melanoma Veterinary Sciences cancer dogs oral melanoma oncology cytokine immunotherapy |
| title | Evaluation of Cytokine Profile in Canine Malignant Oral Melanoma |
| title_full | Evaluation of Cytokine Profile in Canine Malignant Oral Melanoma |
| title_fullStr | Evaluation of Cytokine Profile in Canine Malignant Oral Melanoma |
| title_full_unstemmed | Evaluation of Cytokine Profile in Canine Malignant Oral Melanoma |
| title_short | Evaluation of Cytokine Profile in Canine Malignant Oral Melanoma |
| title_sort | evaluation of cytokine profile in canine malignant oral melanoma |
| topic | cancer dogs oral melanoma oncology cytokine immunotherapy |
| url | https://www.mdpi.com/2306-7381/12/7/627 |
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