Isolation and identification of a red pigment producer endophytic fungus Monascus sanguineus from Rehmannia glutinosa Libosch
Fungal pigments are the potential resources as a natural food colorant. An endophytic fungus RJL03, which is able to produce abundant soluble red pigments, was isolated from the medicinal plant Rehmannia glutinosa Libosch. For further research and utilization of this strain and its secondary metabol...
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Zhejiang University Press
2019-02-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.02.022 |
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| author | HE Yatao GAO Dandan GAN Senning SUN Ting CAI Kuizheng LIU Junlin |
| author_facet | HE Yatao GAO Dandan GAN Senning SUN Ting CAI Kuizheng LIU Junlin |
| author_sort | HE Yatao |
| collection | DOAJ |
| description | Fungal pigments are the potential resources as a natural food colorant. An endophytic fungus RJL03, which is able to produce abundant soluble red pigments, was isolated from the medicinal plant Rehmannia glutinosa Libosch. For further research and utilization of this strain and its secondary metabolites, morphological and molecular characteristics of RJL03 were identified. For observing the colonial morphology, the strain was cultured on different substrates at 25℃ for seven days. The fungus was also observed through light microscope and scanning electron microscope. The colony of this fungus was white at first and turned red at later stage, villous, circular; the mycelium was composed of irregularly branched, septate; conidia had borne terminally on pedicels, single or 2-10 in chain, which was about (8.0-16.5) μm×(7.5-14.0) μm; the cleistothecia was globose, brown, arising terminally from the apex of short hyphae, whose diameter was 32-70 μm; the ascospore was ellipsoidal, smooth, colorless or red, which was about (6.0-7.5) μm×(4.0-5.0) μm. Sequence analysis showed that ITS and 18S of the strain had 100% and 93% similarities with Monascus sanguineus, respectively. In conclusion, morphological results and phylogenetic analysis provided great evidence that endophytic fungus RJL03 was M. sanguineus. This study first reported M. sanguineus isolates from Rehmannia glutinosa Libosch. in China. It is considered that M. sanguineus, unlike M. purpureus, should be an independent species. |
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| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2019-02-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-1eb861c192f94ec9928d8c11f55da3bb2025-08-20T03:16:03ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552019-02-01451710.3785/j.issn.1008-9209.2018.02.02210089209Isolation and identification of a red pigment producer endophytic fungus Monascus sanguineus from Rehmannia glutinosa LiboschHE YataoGAO DandanGAN SenningSUN TingCAI KuizhengLIU JunlinFungal pigments are the potential resources as a natural food colorant. An endophytic fungus RJL03, which is able to produce abundant soluble red pigments, was isolated from the medicinal plant Rehmannia glutinosa Libosch. For further research and utilization of this strain and its secondary metabolites, morphological and molecular characteristics of RJL03 were identified. For observing the colonial morphology, the strain was cultured on different substrates at 25℃ for seven days. The fungus was also observed through light microscope and scanning electron microscope. The colony of this fungus was white at first and turned red at later stage, villous, circular; the mycelium was composed of irregularly branched, septate; conidia had borne terminally on pedicels, single or 2-10 in chain, which was about (8.0-16.5) μm×(7.5-14.0) μm; the cleistothecia was globose, brown, arising terminally from the apex of short hyphae, whose diameter was 32-70 μm; the ascospore was ellipsoidal, smooth, colorless or red, which was about (6.0-7.5) μm×(4.0-5.0) μm. Sequence analysis showed that ITS and 18S of the strain had 100% and 93% similarities with Monascus sanguineus, respectively. In conclusion, morphological results and phylogenetic analysis provided great evidence that endophytic fungus RJL03 was M. sanguineus. This study first reported M. sanguineus isolates from Rehmannia glutinosa Libosch. in China. It is considered that M. sanguineus, unlike M. purpureus, should be an independent species.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.02.022endophytic fungired pigment<italic>Rehmannia glutinosa</italic> Libosch.<italic>Monascus</italic>isolation and identification |
| spellingShingle | HE Yatao GAO Dandan GAN Senning SUN Ting CAI Kuizheng LIU Junlin Isolation and identification of a red pigment producer endophytic fungus Monascus sanguineus from Rehmannia glutinosa Libosch 浙江大学学报. 农业与生命科学版 endophytic fungi red pigment <italic>Rehmannia glutinosa</italic> Libosch. <italic>Monascus</italic> isolation and identification |
| title | Isolation and identification of a red pigment producer endophytic fungus Monascus sanguineus from Rehmannia glutinosa Libosch |
| title_full | Isolation and identification of a red pigment producer endophytic fungus Monascus sanguineus from Rehmannia glutinosa Libosch |
| title_fullStr | Isolation and identification of a red pigment producer endophytic fungus Monascus sanguineus from Rehmannia glutinosa Libosch |
| title_full_unstemmed | Isolation and identification of a red pigment producer endophytic fungus Monascus sanguineus from Rehmannia glutinosa Libosch |
| title_short | Isolation and identification of a red pigment producer endophytic fungus Monascus sanguineus from Rehmannia glutinosa Libosch |
| title_sort | isolation and identification of a red pigment producer endophytic fungus monascus sanguineus from rehmannia glutinosa libosch |
| topic | endophytic fungi red pigment <italic>Rehmannia glutinosa</italic> Libosch. <italic>Monascus</italic> isolation and identification |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.02.022 |
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