Exploring the biological activity and setting dynamics of a novel polydopamine-based root repair material
Background: Root perforation represents a significant complication in endodontics, compromising the structural integrity of the tooth. Effective repair are critical to mitigating the associated risks and preserving long-term dental function. Despite extensive research, the ideal repair material that...
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| Format: | Article |
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Elsevier
2025-07-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426825000867 |
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| author | Lakshmi Nidhi Rao Aditya Shetty Arun M. Isloor Sooraj Nayak Mohana Kumar Jayaprakasha Shetty K Muttanna Venkatesh |
| author_facet | Lakshmi Nidhi Rao Aditya Shetty Arun M. Isloor Sooraj Nayak Mohana Kumar Jayaprakasha Shetty K Muttanna Venkatesh |
| author_sort | Lakshmi Nidhi Rao |
| collection | DOAJ |
| description | Background: Root perforation represents a significant complication in endodontics, compromising the structural integrity of the tooth. Effective repair are critical to mitigating the associated risks and preserving long-term dental function. Despite extensive research, the ideal repair material that fulfills all requisite biological and mechanical properties remains elusive. Methodology: This study introduces a novel polydopamine based repair material designed to address the limitations of existing materials. The material was characterized using field emission scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, transmission electron microscopy, zeta potential, and particle size analysis. The material's initial and final setting times were evaluated using a Gilmore needle apparatus. Biocompatibility was assessed via the MTT assay, while bioactivity was quantified through calcium ion release analysis. Comparative evaluations were conducted against ProRoot MTA, a commercially available standard in root repair. Results: The findings revealed no statistically significant differences in cytotoxicity (P > 0.05) between the experimental material and ProRoot MTA, affirming the biocompatibility of both. However, the experimental material demonstrated a significantly faster set (P < 0.001) than ProRoot MTA, a critical advantage in clinical applications. the inclusion of calcium chloride and bioactive glass enhanced calcium ion release, with the experimental material showing significantly higher levels at 7 days. Conclusion: The polydopamine-bioactive glass composite exhibited favorable biological activity, superior setting dynamics, and enhanced bioactivity, positioning it as a promising candidate for perforation repair. These results lay the groundwork for further research and potential clinical translation, offering a viable solution to a longstanding challenge in endodontics. |
| format | Article |
| id | doaj-art-1eb45e85047243d19e110a2b8c64efb5 |
| institution | DOAJ |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-1eb45e85047243d19e110a2b8c64efb52025-08-20T03:02:10ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-07-0115473774310.1016/j.jobcr.2025.04.001Exploring the biological activity and setting dynamics of a novel polydopamine-based root repair materialLakshmi Nidhi Rao0Aditya Shetty1Arun M. Isloor2Sooraj Nayak3Mohana Kumar4Jayaprakasha Shetty K5Muttanna Venkatesh6Department of Conservative Dentistry and Endodontics, A.B.Shetty Memorial Institute of Dental Sciences, Nitte Deemed to Be University, Deralakatte, Mangalore, 575018, IndiaDepartment of Conservative Dentistry and Endodontics, A.B.Shetty Memorial Institute of Dental Sciences, Nitte Deemed to Be University, Deralakatte, Mangalore, 575018, IndiaMembrane and Separation Technology Laboratory, Department of Chemistry, National Institute of Technology Karnataka, Surathkal, Mangalore, 575 025, IndiaMembrane and Separation Technology Laboratory, Department of Chemistry, National Institute of Technology Karnataka, Surathkal, Mangalore, 575 025, IndiaNitte University Centre for Stem Cell Research and Regenerative Medicine, K.S Hegde Medical Academy, Nitte Deemed to Be University, Deralakatte, Mangalore, 575018, IndiaNitte University Centre for Stem Cell Research and Regenerative Medicine, K.S Hegde Medical Academy, Nitte Deemed to Be University, Deralakatte, Mangalore, 575018, IndiaMembrane and Separation Technology Laboratory, Department of Chemistry, National Institute of Technology Karnataka, Surathkal, Mangalore, 575 025, IndiaBackground: Root perforation represents a significant complication in endodontics, compromising the structural integrity of the tooth. Effective repair are critical to mitigating the associated risks and preserving long-term dental function. Despite extensive research, the ideal repair material that fulfills all requisite biological and mechanical properties remains elusive. Methodology: This study introduces a novel polydopamine based repair material designed to address the limitations of existing materials. The material was characterized using field emission scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, transmission electron microscopy, zeta potential, and particle size analysis. The material's initial and final setting times were evaluated using a Gilmore needle apparatus. Biocompatibility was assessed via the MTT assay, while bioactivity was quantified through calcium ion release analysis. Comparative evaluations were conducted against ProRoot MTA, a commercially available standard in root repair. Results: The findings revealed no statistically significant differences in cytotoxicity (P > 0.05) between the experimental material and ProRoot MTA, affirming the biocompatibility of both. However, the experimental material demonstrated a significantly faster set (P < 0.001) than ProRoot MTA, a critical advantage in clinical applications. the inclusion of calcium chloride and bioactive glass enhanced calcium ion release, with the experimental material showing significantly higher levels at 7 days. Conclusion: The polydopamine-bioactive glass composite exhibited favorable biological activity, superior setting dynamics, and enhanced bioactivity, positioning it as a promising candidate for perforation repair. These results lay the groundwork for further research and potential clinical translation, offering a viable solution to a longstanding challenge in endodontics.http://www.sciencedirect.com/science/article/pii/S2212426825000867Root perforation repairPolydopamineBioactive glassBiocompatibilityEndodontic materials |
| spellingShingle | Lakshmi Nidhi Rao Aditya Shetty Arun M. Isloor Sooraj Nayak Mohana Kumar Jayaprakasha Shetty K Muttanna Venkatesh Exploring the biological activity and setting dynamics of a novel polydopamine-based root repair material Journal of Oral Biology and Craniofacial Research Root perforation repair Polydopamine Bioactive glass Biocompatibility Endodontic materials |
| title | Exploring the biological activity and setting dynamics of a novel polydopamine-based root repair material |
| title_full | Exploring the biological activity and setting dynamics of a novel polydopamine-based root repair material |
| title_fullStr | Exploring the biological activity and setting dynamics of a novel polydopamine-based root repair material |
| title_full_unstemmed | Exploring the biological activity and setting dynamics of a novel polydopamine-based root repair material |
| title_short | Exploring the biological activity and setting dynamics of a novel polydopamine-based root repair material |
| title_sort | exploring the biological activity and setting dynamics of a novel polydopamine based root repair material |
| topic | Root perforation repair Polydopamine Bioactive glass Biocompatibility Endodontic materials |
| url | http://www.sciencedirect.com/science/article/pii/S2212426825000867 |
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