Gut microbiota from Mori fructus (Morus alba L.) polyphenols and polysaccharides-dosed mice activates the PPARα/PGC-1α signaling pathway to mitigate HFD-induced metabolic syndrome in mice

Abstract Mori Fructus, rich in polysaccharides and polyphenols, has long been used in East Asia as a functional food and medicinal agent. In traditional Chinese medicine, it is used to treat various ailments like wasting-thirst syndrome and constipation. Studies suggest its extract fractions may all...

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Main Authors: Meixia Wan, Qing Li, Yudie Xiao, Dan Zhou, Qianya Lei, Shu Wang
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-13715-8
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author Meixia Wan
Qing Li
Yudie Xiao
Dan Zhou
Qianya Lei
Shu Wang
author_facet Meixia Wan
Qing Li
Yudie Xiao
Dan Zhou
Qianya Lei
Shu Wang
author_sort Meixia Wan
collection DOAJ
description Abstract Mori Fructus, rich in polysaccharides and polyphenols, has long been used in East Asia as a functional food and medicinal agent. In traditional Chinese medicine, it is used to treat various ailments like wasting-thirst syndrome and constipation. Studies suggest its extract fractions may alleviate metabolic syndrome symptoms by affecting gut microbiota. To explore this, fecal microbiota transplantation (FMT) was used in an experiment. Pseudo-germ-free mice were created with antibiotics and given a high-fat, high-sugar diet (HFD) to induce metabolic syndrome. Then, fecal bacterial infusions were transplanted. Results showed significant improvement in metabolic syndrome parameters in the FMT-MFPS(fecal microbiota transplantation-Mori Fructus polyphenols plus polysaccharides) group. Dyslipidemia, liver, and kidney injuries were modulated in treated mice. The PPARα/PGC-1α signaling pathway was activated. These findings indicate Mori Fructus extract fractions prevent metabolic syndrome via gut microbiota modulation, with effects sustained through FMT, providing a reference for prevention.
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institution Kabale University
issn 2045-2322
language English
publishDate 2025-08-01
publisher Nature Portfolio
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series Scientific Reports
spelling doaj-art-1ea52d44fbc94457bf4b0e507dac9aa32025-08-20T04:03:02ZengNature PortfolioScientific Reports2045-23222025-08-0115111210.1038/s41598-025-13715-8Gut microbiota from Mori fructus (Morus alba L.) polyphenols and polysaccharides-dosed mice activates the PPARα/PGC-1α signaling pathway to mitigate HFD-induced metabolic syndrome in miceMeixia Wan0Qing Li1Yudie Xiao2Dan Zhou3Qianya Lei4Shu Wang5Medicine School, Longdong UniversityWest China School of Pharmacy, Sichuan UniversityWest China School of Pharmacy, Sichuan UniversityWest China School of Pharmacy, Sichuan UniversityWest China School of Pharmacy, Sichuan UniversityWest China School of Pharmacy, Sichuan UniversityAbstract Mori Fructus, rich in polysaccharides and polyphenols, has long been used in East Asia as a functional food and medicinal agent. In traditional Chinese medicine, it is used to treat various ailments like wasting-thirst syndrome and constipation. Studies suggest its extract fractions may alleviate metabolic syndrome symptoms by affecting gut microbiota. To explore this, fecal microbiota transplantation (FMT) was used in an experiment. Pseudo-germ-free mice were created with antibiotics and given a high-fat, high-sugar diet (HFD) to induce metabolic syndrome. Then, fecal bacterial infusions were transplanted. Results showed significant improvement in metabolic syndrome parameters in the FMT-MFPS(fecal microbiota transplantation-Mori Fructus polyphenols plus polysaccharides) group. Dyslipidemia, liver, and kidney injuries were modulated in treated mice. The PPARα/PGC-1α signaling pathway was activated. These findings indicate Mori Fructus extract fractions prevent metabolic syndrome via gut microbiota modulation, with effects sustained through FMT, providing a reference for prevention.https://doi.org/10.1038/s41598-025-13715-8Mori fructus (Morus Alba L.)Metabolic syndromeFecal microbiota transplantationPPARα/PGC-1α signaling pathway
spellingShingle Meixia Wan
Qing Li
Yudie Xiao
Dan Zhou
Qianya Lei
Shu Wang
Gut microbiota from Mori fructus (Morus alba L.) polyphenols and polysaccharides-dosed mice activates the PPARα/PGC-1α signaling pathway to mitigate HFD-induced metabolic syndrome in mice
Scientific Reports
Mori fructus (Morus Alba L.)
Metabolic syndrome
Fecal microbiota transplantation
PPARα/PGC-1α signaling pathway
title Gut microbiota from Mori fructus (Morus alba L.) polyphenols and polysaccharides-dosed mice activates the PPARα/PGC-1α signaling pathway to mitigate HFD-induced metabolic syndrome in mice
title_full Gut microbiota from Mori fructus (Morus alba L.) polyphenols and polysaccharides-dosed mice activates the PPARα/PGC-1α signaling pathway to mitigate HFD-induced metabolic syndrome in mice
title_fullStr Gut microbiota from Mori fructus (Morus alba L.) polyphenols and polysaccharides-dosed mice activates the PPARα/PGC-1α signaling pathway to mitigate HFD-induced metabolic syndrome in mice
title_full_unstemmed Gut microbiota from Mori fructus (Morus alba L.) polyphenols and polysaccharides-dosed mice activates the PPARα/PGC-1α signaling pathway to mitigate HFD-induced metabolic syndrome in mice
title_short Gut microbiota from Mori fructus (Morus alba L.) polyphenols and polysaccharides-dosed mice activates the PPARα/PGC-1α signaling pathway to mitigate HFD-induced metabolic syndrome in mice
title_sort gut microbiota from mori fructus morus alba l polyphenols and polysaccharides dosed mice activates the pparα pgc 1α signaling pathway to mitigate hfd induced metabolic syndrome in mice
topic Mori fructus (Morus Alba L.)
Metabolic syndrome
Fecal microbiota transplantation
PPARα/PGC-1α signaling pathway
url https://doi.org/10.1038/s41598-025-13715-8
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