Detection of <i>Trypanosoma cruzi</i> in a Reactive Blood Bank Sample in Sonora, Mexico
Chagas disease is a neglected disease caused by the parasite <i>Trypanosoma cruzi</i>, a public health problem in both endemic and non-endemic countries. In Mexico, the southern region is considered endemic, and cases are frequently reported; however, in the northwestern region, only a f...
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MDPI AG
2025-04-01
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| Online Access: | https://www.mdpi.com/2414-6366/10/4/104 |
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| author | Idalia Paredes-Sotelo Mónica Reséndiz-Sandoval Adriana Garibay-Escobar Edgar Alfonso Paredes-González Aracely Angulo-Molina Angel Ramos-Ligonio Eric Dumonteil Claudia Herrera Olivia Valenzuela |
| author_facet | Idalia Paredes-Sotelo Mónica Reséndiz-Sandoval Adriana Garibay-Escobar Edgar Alfonso Paredes-González Aracely Angulo-Molina Angel Ramos-Ligonio Eric Dumonteil Claudia Herrera Olivia Valenzuela |
| author_sort | Idalia Paredes-Sotelo |
| collection | DOAJ |
| description | Chagas disease is a neglected disease caused by the parasite <i>Trypanosoma cruzi</i>, a public health problem in both endemic and non-endemic countries. In Mexico, the southern region is considered endemic, and cases are frequently reported; however, in the northwestern region, only a few cases are confirmed annually. This study describes, for the first time, the Discrete Typing Unit (DTU) of <i>Trypanosoma cruzi</i> in a volunteer blood donor rejected for being reactive in the northwestern region of Mexico. Seroreactivity was confirmed using “in-house” ELISAs which employed three different antigens: total extract from <i>Trypanosoma cruzi</i> isolated from a vector (<i>Triatoma rubida</i>) from Sonora (strain T1), strain H1 and CL-Brener. The molecular characterization of <i>Trypanosoma cruzi</i> was conducted by amplifying satellite DNA by qPCR and posterior sequencing of the mini-exon gene, using Next Generation Sequencing (NGS) to enhance the accuracy of genetic characterization. The results show that the reactive status of this blood donor was confirmed using our in-house ELISAs, and the presence of <i>Trypanosoma cruzi</i> by detecting TcI DTU confirmed the infection status. |
| format | Article |
| id | doaj-art-1ea13c4fecac45949a85c67f80522f84 |
| institution | OA Journals |
| issn | 2414-6366 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Tropical Medicine and Infectious Disease |
| spelling | doaj-art-1ea13c4fecac45949a85c67f80522f842025-08-20T02:25:12ZengMDPI AGTropical Medicine and Infectious Disease2414-63662025-04-0110410410.3390/tropicalmed10040104Detection of <i>Trypanosoma cruzi</i> in a Reactive Blood Bank Sample in Sonora, MexicoIdalia Paredes-Sotelo0Mónica Reséndiz-Sandoval1Adriana Garibay-Escobar2Edgar Alfonso Paredes-González3Aracely Angulo-Molina4Angel Ramos-Ligonio5Eric Dumonteil6Claudia Herrera7Olivia Valenzuela8Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, MexicoDepartamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, MexicoDepartamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, MexicoDepartamento de Ciencias Químico Biológicas y Agropecuarias, Universidad de Sonora, Caborca C.P. 83600, Sonora, MexicoDepartamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, MexicoLADISER, Inmunología y Biología Molecular, Facultad de Ciencias Químicas, Universidad Veracruzana, Orizaba C.P. 94340, Veracruz, MexicoDepartment of Tropical Medicine and Infectious Disease, Celia Scott Weatherhead School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USADepartment of Tropical Medicine and Infectious Disease, Celia Scott Weatherhead School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USADepartamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo C.P. 83000, Sonora, MexicoChagas disease is a neglected disease caused by the parasite <i>Trypanosoma cruzi</i>, a public health problem in both endemic and non-endemic countries. In Mexico, the southern region is considered endemic, and cases are frequently reported; however, in the northwestern region, only a few cases are confirmed annually. This study describes, for the first time, the Discrete Typing Unit (DTU) of <i>Trypanosoma cruzi</i> in a volunteer blood donor rejected for being reactive in the northwestern region of Mexico. Seroreactivity was confirmed using “in-house” ELISAs which employed three different antigens: total extract from <i>Trypanosoma cruzi</i> isolated from a vector (<i>Triatoma rubida</i>) from Sonora (strain T1), strain H1 and CL-Brener. The molecular characterization of <i>Trypanosoma cruzi</i> was conducted by amplifying satellite DNA by qPCR and posterior sequencing of the mini-exon gene, using Next Generation Sequencing (NGS) to enhance the accuracy of genetic characterization. The results show that the reactive status of this blood donor was confirmed using our in-house ELISAs, and the presence of <i>Trypanosoma cruzi</i> by detecting TcI DTU confirmed the infection status.https://www.mdpi.com/2414-6366/10/4/104<i>Trypanosoma cruzi</i>Chagas diseaseblood bankserologymolecular diagnosticsdiscrete typing unit |
| spellingShingle | Idalia Paredes-Sotelo Mónica Reséndiz-Sandoval Adriana Garibay-Escobar Edgar Alfonso Paredes-González Aracely Angulo-Molina Angel Ramos-Ligonio Eric Dumonteil Claudia Herrera Olivia Valenzuela Detection of <i>Trypanosoma cruzi</i> in a Reactive Blood Bank Sample in Sonora, Mexico Tropical Medicine and Infectious Disease <i>Trypanosoma cruzi</i> Chagas disease blood bank serology molecular diagnostics discrete typing unit |
| title | Detection of <i>Trypanosoma cruzi</i> in a Reactive Blood Bank Sample in Sonora, Mexico |
| title_full | Detection of <i>Trypanosoma cruzi</i> in a Reactive Blood Bank Sample in Sonora, Mexico |
| title_fullStr | Detection of <i>Trypanosoma cruzi</i> in a Reactive Blood Bank Sample in Sonora, Mexico |
| title_full_unstemmed | Detection of <i>Trypanosoma cruzi</i> in a Reactive Blood Bank Sample in Sonora, Mexico |
| title_short | Detection of <i>Trypanosoma cruzi</i> in a Reactive Blood Bank Sample in Sonora, Mexico |
| title_sort | detection of i trypanosoma cruzi i in a reactive blood bank sample in sonora mexico |
| topic | <i>Trypanosoma cruzi</i> Chagas disease blood bank serology molecular diagnostics discrete typing unit |
| url | https://www.mdpi.com/2414-6366/10/4/104 |
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