A Single-Tube Two-Step MIRA-CRISPR/Cas12b Assay for the Rapid Detection of Mpox Virus

A Single-Tube Two-Step MIRA-CRISPR/Cas12b Assay for the Rapid Detection of Mpox Virus

Mpox is a zoonotic disease caused by the Mpox virus (MPXV). The rapid and accurate diagnosis of MPXV is essential for the timely and effective prevention, control, and treatment of the disease. In this study, we combined Multienzyme Isothermal Rapid Amplification (MIRA) (at 42 °C) and Clustered Regu...

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Bibliographic Details
Main Authors: Ge Hu, Zhijie Wei, Jinlei Guo, Kangchen Zhao, Qiao Qiao, Xiaojuan Zhu, Tao Wu, Heng Rong, Shuo Ning, Ziyang Hao, Ying Chi, Lunbiao Cui, Yiyue Ge
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Viruses
Subjects:
Mpox
MIRA
CRISPR/Cas12b
tricosane
Online Access:https://www.mdpi.com/1999-4915/17/6/841
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