Association of SIRT1 rs3758391 Polymorphism With T2DM in Bangladeshi Population: Evidence From a Case‐Control Study and Meta‐Analysis

ABSTRACT Background and Aim Type 2 diabetes mellitus (T2DM) remains one of the major causes of morbidity and mortality worldwide, including Bangladesh. SIRT1, an NAD‐dependent deacetylase, is involved in energy homeostasis and protects β‐cells of the pancreas from oxidative stress. Single nucleotide...

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Main Authors: Rezwana Ahmed, Mushfikur Rahman Safa, Zahidul Islam Zahid, Md. Mazharul Islam Chowdhury, A.B.M. Kamrul Hasan, Md. Shaki Mostaid, Hasan Mahmud Reza
Format: Article
Language:English
Published: Wiley 2025-02-01
Series:Health Science Reports
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Online Access:https://doi.org/10.1002/hsr2.70495
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author Rezwana Ahmed
Mushfikur Rahman Safa
Zahidul Islam Zahid
Md. Mazharul Islam Chowdhury
A.B.M. Kamrul Hasan
Md. Shaki Mostaid
Hasan Mahmud Reza
author_facet Rezwana Ahmed
Mushfikur Rahman Safa
Zahidul Islam Zahid
Md. Mazharul Islam Chowdhury
A.B.M. Kamrul Hasan
Md. Shaki Mostaid
Hasan Mahmud Reza
author_sort Rezwana Ahmed
collection DOAJ
description ABSTRACT Background and Aim Type 2 diabetes mellitus (T2DM) remains one of the major causes of morbidity and mortality worldwide, including Bangladesh. SIRT1, an NAD‐dependent deacetylase, is involved in energy homeostasis and protects β‐cells of the pancreas from oxidative stress. Single nucleotide polymorphisms (SNPs) in the SIRT1 gene have been found to be associated with T2DM in several populations, however, with conflicting results. The aim of this present case‐control study, along with the meta‐analysis, was to elucidate the association of rs3758391 polymorphism with the susceptibility to T2DM in Bangladeshi population. Methods 72 T2DM patients and 90 healthy controls were enrolled in our study and polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) was employed for genotyping the SNP. Odds ratio (OR) with 95% confidence interval (95% CI) was used to represent the association of SIRT1 polymorphism with T2DM. For the meta‐analysis six studies were included and pooled odds ratio with 95% CI were calculated for six genetic models using the random effects model. Heterogeneity and publication bias was also calculated for each study. Results A significant association was found between rs3758391 polymorphism and increased risk of T2DM under codominant TT versus CC (OR = 3.88, 95% CI = 1.34–11.25, p = 0.012), recessive TT versus CC + CT (OR = 2.83, 95% CI = 1.12–7.09, p = 0.027) and allelic T versus C (OR = 1.67, 95% CI = 1.07–2.60, p = 0.024) genetic models. However, no significant association between rs3758391 and other biochemical and anthropometric parameters were found. Our meta‐analysis showed no statistically significant association of this polymorphism. Conclusion We conclude that, polymorphism at rs3758391 of SIRT1 gene conferred an increased risk of T2DM in Bangladeshi population.
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spelling doaj-art-1e9bfca591364f0fb29cd80d7828bae32025-08-20T02:55:06ZengWileyHealth Science Reports2398-88352025-02-0182n/an/a10.1002/hsr2.70495Association of SIRT1 rs3758391 Polymorphism With T2DM in Bangladeshi Population: Evidence From a Case‐Control Study and Meta‐AnalysisRezwana Ahmed0Mushfikur Rahman Safa1Zahidul Islam Zahid2Md. Mazharul Islam Chowdhury3A.B.M. Kamrul Hasan4Md. Shaki Mostaid5Hasan Mahmud Reza6Department of Pharmaceutical Sciences North South University Dhaka BangladeshDepartment of Pharmaceutical Sciences North South University Dhaka BangladeshDepartment of Pharmaceutical Sciences North South University Dhaka BangladeshDepartment of Pharmaceutical Sciences North South University Dhaka BangladeshDepartment of Endocrinology Mymensingh Medical College Mymensingh BangladeshDepartment of Pharmaceutical Sciences North South University Dhaka BangladeshDepartment of Pharmaceutical Sciences North South University Dhaka BangladeshABSTRACT Background and Aim Type 2 diabetes mellitus (T2DM) remains one of the major causes of morbidity and mortality worldwide, including Bangladesh. SIRT1, an NAD‐dependent deacetylase, is involved in energy homeostasis and protects β‐cells of the pancreas from oxidative stress. Single nucleotide polymorphisms (SNPs) in the SIRT1 gene have been found to be associated with T2DM in several populations, however, with conflicting results. The aim of this present case‐control study, along with the meta‐analysis, was to elucidate the association of rs3758391 polymorphism with the susceptibility to T2DM in Bangladeshi population. Methods 72 T2DM patients and 90 healthy controls were enrolled in our study and polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) was employed for genotyping the SNP. Odds ratio (OR) with 95% confidence interval (95% CI) was used to represent the association of SIRT1 polymorphism with T2DM. For the meta‐analysis six studies were included and pooled odds ratio with 95% CI were calculated for six genetic models using the random effects model. Heterogeneity and publication bias was also calculated for each study. Results A significant association was found between rs3758391 polymorphism and increased risk of T2DM under codominant TT versus CC (OR = 3.88, 95% CI = 1.34–11.25, p = 0.012), recessive TT versus CC + CT (OR = 2.83, 95% CI = 1.12–7.09, p = 0.027) and allelic T versus C (OR = 1.67, 95% CI = 1.07–2.60, p = 0.024) genetic models. However, no significant association between rs3758391 and other biochemical and anthropometric parameters were found. Our meta‐analysis showed no statistically significant association of this polymorphism. Conclusion We conclude that, polymorphism at rs3758391 of SIRT1 gene conferred an increased risk of T2DM in Bangladeshi population.https://doi.org/10.1002/hsr2.70495Bangladeshriskrs3578391SIRT1type 2 diabetes mellitus
spellingShingle Rezwana Ahmed
Mushfikur Rahman Safa
Zahidul Islam Zahid
Md. Mazharul Islam Chowdhury
A.B.M. Kamrul Hasan
Md. Shaki Mostaid
Hasan Mahmud Reza
Association of SIRT1 rs3758391 Polymorphism With T2DM in Bangladeshi Population: Evidence From a Case‐Control Study and Meta‐Analysis
Health Science Reports
Bangladesh
risk
rs3578391
SIRT1
type 2 diabetes mellitus
title Association of SIRT1 rs3758391 Polymorphism With T2DM in Bangladeshi Population: Evidence From a Case‐Control Study and Meta‐Analysis
title_full Association of SIRT1 rs3758391 Polymorphism With T2DM in Bangladeshi Population: Evidence From a Case‐Control Study and Meta‐Analysis
title_fullStr Association of SIRT1 rs3758391 Polymorphism With T2DM in Bangladeshi Population: Evidence From a Case‐Control Study and Meta‐Analysis
title_full_unstemmed Association of SIRT1 rs3758391 Polymorphism With T2DM in Bangladeshi Population: Evidence From a Case‐Control Study and Meta‐Analysis
title_short Association of SIRT1 rs3758391 Polymorphism With T2DM in Bangladeshi Population: Evidence From a Case‐Control Study and Meta‐Analysis
title_sort association of sirt1 rs3758391 polymorphism with t2dm in bangladeshi population evidence from a case control study and meta analysis
topic Bangladesh
risk
rs3578391
SIRT1
type 2 diabetes mellitus
url https://doi.org/10.1002/hsr2.70495
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