Transmittance Characterization of Objective Lenses Covering all Four Near Infrared Optical Windows and its Application to Three-Photon Microscopy Excited at 1820 nm
Near infrared (NIR) excitation or emission is capable of deep-tissue penetration in various modalities of optical microscopy. Based on the transmittance characterization of biological tissue, such as brains, four NIR optical windows have been demonstrated or suggested: The 800-nm, the 1300-nm, the 1...
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IEEE
2018-01-01
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| Series: | IEEE Photonics Journal |
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| Online Access: | https://ieeexplore.ieee.org/document/8352115/ |
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| author | Ke Wang Wenhui Wen Hongji Liu Yu Du Ziwei Zhuang Ping Qiu |
| author_facet | Ke Wang Wenhui Wen Hongji Liu Yu Du Ziwei Zhuang Ping Qiu |
| author_sort | Ke Wang |
| collection | DOAJ |
| description | Near infrared (NIR) excitation or emission is capable of deep-tissue penetration in various modalities of optical microscopy. Based on the transmittance characterization of biological tissue, such as brains, four NIR optical windows have been demonstrated or suggested: The 800-nm, the 1300-nm, the 1700-nm, and the 2200-nm window. High-numerical aperture objective lenses are needed in optical microscopy to both deliver sufficient excitation light and collect efficient signal light to enable deep-tissue imaging. The transmittance performances of objective lenses are of vital importance. However, there is a lack of experimental characterization of the transmittance, especially at long wavelengths, which poses a dramatic obstacle for lens selection in imaging experiments. Here, we demonstrate detailed measurement results of the transmittance performance of air, water-immersion, and oil-immersion objectives available to us, covering all the four NIR optical windows. These results will provide direct guidelines for objective lens selection in terms of transmittance performance. We further demonstrate three-photon microscopy with 1820-nm excitation, close to the edge of the 1700-nm window, using the objective lens based on our transmittance measurement. |
| format | Article |
| id | doaj-art-1e9054364c7b488e9e56aa41583e2f09 |
| institution | DOAJ |
| issn | 1943-0655 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Photonics Journal |
| spelling | doaj-art-1e9054364c7b488e9e56aa41583e2f092025-08-20T02:44:40ZengIEEEIEEE Photonics Journal1943-06552018-01-011031710.1109/JPHOT.2018.28284358352115Transmittance Characterization of Objective Lenses Covering all Four Near Infrared Optical Windows and its Application to Three-Photon Microscopy Excited at 1820 nmKe Wang0https://orcid.org/0000-0002-5102-5465Wenhui Wen1Hongji Liu2Yu Du3Ziwei Zhuang4Ping Qiu5https://orcid.org/0000-0002-2114-5909Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaCollege of Physics and Energy, Shenzhen University, Shenzhen, ChinaNear infrared (NIR) excitation or emission is capable of deep-tissue penetration in various modalities of optical microscopy. Based on the transmittance characterization of biological tissue, such as brains, four NIR optical windows have been demonstrated or suggested: The 800-nm, the 1300-nm, the 1700-nm, and the 2200-nm window. High-numerical aperture objective lenses are needed in optical microscopy to both deliver sufficient excitation light and collect efficient signal light to enable deep-tissue imaging. The transmittance performances of objective lenses are of vital importance. However, there is a lack of experimental characterization of the transmittance, especially at long wavelengths, which poses a dramatic obstacle for lens selection in imaging experiments. Here, we demonstrate detailed measurement results of the transmittance performance of air, water-immersion, and oil-immersion objectives available to us, covering all the four NIR optical windows. These results will provide direct guidelines for objective lens selection in terms of transmittance performance. We further demonstrate three-photon microscopy with 1820-nm excitation, close to the edge of the 1700-nm window, using the objective lens based on our transmittance measurement.https://ieeexplore.ieee.org/document/8352115/Fluorescence microscopynonlinear microscopymultiphoton processes |
| spellingShingle | Ke Wang Wenhui Wen Hongji Liu Yu Du Ziwei Zhuang Ping Qiu Transmittance Characterization of Objective Lenses Covering all Four Near Infrared Optical Windows and its Application to Three-Photon Microscopy Excited at 1820 nm IEEE Photonics Journal Fluorescence microscopy nonlinear microscopy multiphoton processes |
| title | Transmittance Characterization of Objective Lenses Covering all Four Near Infrared Optical Windows and its Application to Three-Photon Microscopy Excited at 1820 nm |
| title_full | Transmittance Characterization of Objective Lenses Covering all Four Near Infrared Optical Windows and its Application to Three-Photon Microscopy Excited at 1820 nm |
| title_fullStr | Transmittance Characterization of Objective Lenses Covering all Four Near Infrared Optical Windows and its Application to Three-Photon Microscopy Excited at 1820 nm |
| title_full_unstemmed | Transmittance Characterization of Objective Lenses Covering all Four Near Infrared Optical Windows and its Application to Three-Photon Microscopy Excited at 1820 nm |
| title_short | Transmittance Characterization of Objective Lenses Covering all Four Near Infrared Optical Windows and its Application to Three-Photon Microscopy Excited at 1820 nm |
| title_sort | transmittance characterization of objective lenses covering all four near infrared optical windows and its application to three photon microscopy excited at 1820 nm |
| topic | Fluorescence microscopy nonlinear microscopy multiphoton processes |
| url | https://ieeexplore.ieee.org/document/8352115/ |
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