Efficient degradation of neomycin by Bacillus velezensis and Cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewater
Neomycin, an aminoglycoside antibiotic, is widely utilized for veterinary medicine in disease prevention. Biodegradation is a key pathway for the removal of neomycin from the environment. To date, only the white-rot fungus Trametes versicolor and the ericoid mycorrhizal fungus Rhizoscyphus ericae ha...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544888/full |
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| author | Qian Yang Wenzhuan Huang Xue Yan Qiang Ding Jiaxin Liu Bo Cheng Tao Duan |
| author_facet | Qian Yang Wenzhuan Huang Xue Yan Qiang Ding Jiaxin Liu Bo Cheng Tao Duan |
| author_sort | Qian Yang |
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| description | Neomycin, an aminoglycoside antibiotic, is widely utilized for veterinary medicine in disease prevention. Biodegradation is a key pathway for the removal of neomycin from the environment. To date, only the white-rot fungus Trametes versicolor and the ericoid mycorrhizal fungus Rhizoscyphus ericae have been documented to efficiently degrade neomycin. However, no bacterial species with neomycin-degrading capabilities have been reported, underscoring a significant gap in microbial research related to neomycin remediation. In this study, Cupriavidus basilensis and Bacillus velezensis were isolated from pharmaceutical wastewater and neomycin-free mangrove soil through enrichment culture and gradual acclimatization, respectively. These isolates demonstrated neomycin degradation rates of 46.4 and 37.6% in 96 h with 100 mg·L−1 neomycin as the sole carbon source. Cupriavidus basilensis achieved a degradation rate of 50.83% with ammonium sulfate supplementation, while Bacillus velezensis exhibited a superior degradation efficiency of 58.44% with soluble starch. Our findings offer valuable insights into the microbial degradation of neomycin. Two neomycin-degrading bacteria were isolated for the first time. Both species degraded neomycin as the sole carbon source or under co-metabolic conditions within 4 days. Microorganisms from neomycin-free environments adapted to neomycin stress and outperformed those from contaminated sources. This challenges the assumption that antibiotic-degrading microorganisms mainly originate from polluted environments. The findings expand the diversity of known neomycin-degrading microorganisms and demonstrate their potential for removing refractory neomycin from pharmaceutical wastewater. |
| format | Article |
| id | doaj-art-1e8d5e5e816f428283f4545da9b24a00 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-1e8d5e5e816f428283f4545da9b24a002025-01-29T16:57:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.15448881544888Efficient degradation of neomycin by Bacillus velezensis and Cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewaterQian Yang0Wenzhuan Huang1Xue Yan2Qiang Ding3Jiaxin Liu4Bo Cheng5Tao Duan6School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, ChinaSchool of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, ChinaYichang Humanwell Pharmaceutical Co., Ltd., Hubei, ChinaSchool of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, ChinaSchool of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, ChinaSchool of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan, ChinaGuangzhou Higher Education Mega Center, School of Life Science and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaNeomycin, an aminoglycoside antibiotic, is widely utilized for veterinary medicine in disease prevention. Biodegradation is a key pathway for the removal of neomycin from the environment. To date, only the white-rot fungus Trametes versicolor and the ericoid mycorrhizal fungus Rhizoscyphus ericae have been documented to efficiently degrade neomycin. However, no bacterial species with neomycin-degrading capabilities have been reported, underscoring a significant gap in microbial research related to neomycin remediation. In this study, Cupriavidus basilensis and Bacillus velezensis were isolated from pharmaceutical wastewater and neomycin-free mangrove soil through enrichment culture and gradual acclimatization, respectively. These isolates demonstrated neomycin degradation rates of 46.4 and 37.6% in 96 h with 100 mg·L−1 neomycin as the sole carbon source. Cupriavidus basilensis achieved a degradation rate of 50.83% with ammonium sulfate supplementation, while Bacillus velezensis exhibited a superior degradation efficiency of 58.44% with soluble starch. Our findings offer valuable insights into the microbial degradation of neomycin. Two neomycin-degrading bacteria were isolated for the first time. Both species degraded neomycin as the sole carbon source or under co-metabolic conditions within 4 days. Microorganisms from neomycin-free environments adapted to neomycin stress and outperformed those from contaminated sources. This challenges the assumption that antibiotic-degrading microorganisms mainly originate from polluted environments. The findings expand the diversity of known neomycin-degrading microorganisms and demonstrate their potential for removing refractory neomycin from pharmaceutical wastewater.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544888/fullBacillus velezensisCupriavidus basilensisneomycin-free soilneomycinbiodegradation |
| spellingShingle | Qian Yang Wenzhuan Huang Xue Yan Qiang Ding Jiaxin Liu Bo Cheng Tao Duan Efficient degradation of neomycin by Bacillus velezensis and Cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewater Frontiers in Microbiology Bacillus velezensis Cupriavidus basilensis neomycin-free soil neomycin biodegradation |
| title | Efficient degradation of neomycin by Bacillus velezensis and Cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewater |
| title_full | Efficient degradation of neomycin by Bacillus velezensis and Cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewater |
| title_fullStr | Efficient degradation of neomycin by Bacillus velezensis and Cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewater |
| title_full_unstemmed | Efficient degradation of neomycin by Bacillus velezensis and Cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewater |
| title_short | Efficient degradation of neomycin by Bacillus velezensis and Cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewater |
| title_sort | efficient degradation of neomycin by bacillus velezensis and cupriavidus basilensis isolated from mangrove soil and pharmaceutical wastewater |
| topic | Bacillus velezensis Cupriavidus basilensis neomycin-free soil neomycin biodegradation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544888/full |
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