Photoacoustic detecting of brain lymphatic dysfunction in inflammatory models

The brain lymphatic system plays a crucial role in maintaining homeostasis, clearing metabolic waste, and regulating neuroinflammation. Its dysfunction is strongly linked to neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. In this study, we employed dual-contrast funct...

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Main Authors: Meng Wang, Dan Wang, Zhigang Wang, Wenbin Shi, Zhiyang Wang
Format: Article
Language:English
Published: World Scientific Publishing 2025-09-01
Series:Journal of Innovative Optical Health Sciences
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Online Access:https://www.worldscientific.com/doi/10.1142/S1793545825500154
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author Meng Wang
Dan Wang
Zhigang Wang
Wenbin Shi
Zhiyang Wang
author_facet Meng Wang
Dan Wang
Zhigang Wang
Wenbin Shi
Zhiyang Wang
author_sort Meng Wang
collection DOAJ
description The brain lymphatic system plays a crucial role in maintaining homeostasis, clearing metabolic waste, and regulating neuroinflammation. Its dysfunction is strongly linked to neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. In this study, we employed dual-contrast functional photoacoustic microscopy to evaluate the impact of lipopolysaccharide-induced central nervous system inflammation on brain lymphatic function and to explore the protective effects of the P2X7 receptor (P2X7R) antagonist. Our findings demonstrated that lipopolysaccharide intervention led to impaired function of the meningeal lymphatic vessels, which was partially restored by the P2X7R antagonist, whereas its effects on the glymphatic system and cerebral vessels were minimal. This study further supports the feasibility of photoacoustic microscopy for assessing brain lymphatic function and highlights the therapeutic potential of P2X7R antagonism. These findings suggest that P2X7R may serve as a key target for modulating brain-lymphatic interactions, providing an experimental foundation for developing intervention strategies for neuroinflammatory and neurodegenerative diseases.
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publishDate 2025-09-01
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spelling doaj-art-1e810f021e614044a635c3da16fd61ec2025-08-20T02:46:57ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052025-09-01180510.1142/S1793545825500154Photoacoustic detecting of brain lymphatic dysfunction in inflammatory modelsMeng Wang0Dan Wang1Zhigang Wang2Wenbin Shi3Zhiyang Wang4MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaSchool of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, P. R. ChinaMOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaMOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaMOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, P. R. ChinaThe brain lymphatic system plays a crucial role in maintaining homeostasis, clearing metabolic waste, and regulating neuroinflammation. Its dysfunction is strongly linked to neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. In this study, we employed dual-contrast functional photoacoustic microscopy to evaluate the impact of lipopolysaccharide-induced central nervous system inflammation on brain lymphatic function and to explore the protective effects of the P2X7 receptor (P2X7R) antagonist. Our findings demonstrated that lipopolysaccharide intervention led to impaired function of the meningeal lymphatic vessels, which was partially restored by the P2X7R antagonist, whereas its effects on the glymphatic system and cerebral vessels were minimal. This study further supports the feasibility of photoacoustic microscopy for assessing brain lymphatic function and highlights the therapeutic potential of P2X7R antagonism. These findings suggest that P2X7R may serve as a key target for modulating brain-lymphatic interactions, providing an experimental foundation for developing intervention strategies for neuroinflammatory and neurodegenerative diseases.https://www.worldscientific.com/doi/10.1142/S1793545825500154Photoacoustic microscopybrain lymphatic systemneuroinflammationP2X7 receptor
spellingShingle Meng Wang
Dan Wang
Zhigang Wang
Wenbin Shi
Zhiyang Wang
Photoacoustic detecting of brain lymphatic dysfunction in inflammatory models
Journal of Innovative Optical Health Sciences
Photoacoustic microscopy
brain lymphatic system
neuroinflammation
P2X7 receptor
title Photoacoustic detecting of brain lymphatic dysfunction in inflammatory models
title_full Photoacoustic detecting of brain lymphatic dysfunction in inflammatory models
title_fullStr Photoacoustic detecting of brain lymphatic dysfunction in inflammatory models
title_full_unstemmed Photoacoustic detecting of brain lymphatic dysfunction in inflammatory models
title_short Photoacoustic detecting of brain lymphatic dysfunction in inflammatory models
title_sort photoacoustic detecting of brain lymphatic dysfunction in inflammatory models
topic Photoacoustic microscopy
brain lymphatic system
neuroinflammation
P2X7 receptor
url https://www.worldscientific.com/doi/10.1142/S1793545825500154
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AT danwang photoacousticdetectingofbrainlymphaticdysfunctionininflammatorymodels
AT zhigangwang photoacousticdetectingofbrainlymphaticdysfunctionininflammatorymodels
AT wenbinshi photoacousticdetectingofbrainlymphaticdysfunctionininflammatorymodels
AT zhiyangwang photoacousticdetectingofbrainlymphaticdysfunctionininflammatorymodels