The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 breast cancer stem cells
The cancer stem cell (CSC) hypothesis has gained significant recognition in describing tumorigenesis. Identification of the factors critical to development of breast cancer stem cells (BCSCs) may provide insight into the improvement of effective therapies against breast cancer. In this study, we aim...
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| Language: | English |
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SAGE Publishing
2017-04-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317695927 |
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| author | Yonggang Lv Ting Wang Jing Fan Zhenzhen Zhang Juliang Zhang Cheng Xu Yongping Li Ge Zhao Chenyang He Huimin Meng Hua Yang Zhen Wang Jiayun Liu Jianghao Chen Ling Wang |
| author_facet | Yonggang Lv Ting Wang Jing Fan Zhenzhen Zhang Juliang Zhang Cheng Xu Yongping Li Ge Zhao Chenyang He Huimin Meng Hua Yang Zhen Wang Jiayun Liu Jianghao Chen Ling Wang |
| author_sort | Yonggang Lv |
| collection | DOAJ |
| description | The cancer stem cell (CSC) hypothesis has gained significant recognition in describing tumorigenesis. Identification of the factors critical to development of breast cancer stem cells (BCSCs) may provide insight into the improvement of effective therapies against breast cancer. In this study, we aim to investigate the biological function of SLC34A2 in affecting the stem cell-like phenotypes in BCSCs and its underlying mechanisms. We demonstrated that CD147 + cells from breast cancer tissue samples and cell lines possessed BCSC-like features, including the ability of self-renewal in vitro, differentiation, and tumorigenic potential in vivo. Flow cytometry analysis showed the presence of a variable fraction of CD147 + cells in 9 of 10 tumor samples. Significantly, SLC34A2 expression in CD147 + BCSCs was enhanced compared with that in differentiated adherent progeny of CD147 + BCSCs and adherently cultured cell line cells. In breast cancer patient cohorts, SLC34A2 expression was found increased in 9 of 10 tumor samples. By using lentiviral-based approach, si-SLC34A2-transduced CD147 + BCSCs showed decreased ability of sphere formation, cell viability in vitro, and tumorigenicity in vivo, which suggested the essential role of SLC34A2 in CD147 + BCSCs. Furthermore, PI3K/AKT pathway and SOX2 were found necessary to maintain the stemness of CD147 + BCSCs by using LY294002 or lentiviral-si-SOX2. Finally, we indicated that SLC34A2 could regulate SOX2 to maintain the stem cell–like features in CD147 + BCSCs through PI3K/AKT pathway. Therefore, our report identifies a novel role of SLC34A2 in BCSCs’ state regulation and establishes a rationale for targeting the SLC34A2/PI3K/AKT/SOX2 signaling pathway for breast cancer therapy. |
| format | Article |
| id | doaj-art-1e76535c446844ec8651c4cb466f14bf |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-1e76535c446844ec8651c4cb466f14bf2025-08-20T03:32:47ZengSAGE PublishingTumor Biology1423-03802017-04-013910.1177/1010428317695927The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 breast cancer stem cellsYonggang Lv0Ting Wang1Jing Fan2Zhenzhen Zhang3Juliang Zhang4Cheng Xu5Yongping Li6Ge Zhao7Chenyang He8Huimin Meng9Hua Yang10Zhen Wang11Jiayun Liu12Jianghao Chen13Ling Wang14Department of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaBiotecan Medical Diagnostics Co. Ltd., Zhangjiang Center for Translational Medicine, Shanghai, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaBiotecan Medical Diagnostics Co. Ltd., Zhangjiang Center for Translational Medicine, Shanghai, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaDepartment of Orthopaedic, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaInstitute of Clinical Laboratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi’ an, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaDepartment of Vascular and Endocrine Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, People’s Republic of ChinaThe cancer stem cell (CSC) hypothesis has gained significant recognition in describing tumorigenesis. Identification of the factors critical to development of breast cancer stem cells (BCSCs) may provide insight into the improvement of effective therapies against breast cancer. In this study, we aim to investigate the biological function of SLC34A2 in affecting the stem cell-like phenotypes in BCSCs and its underlying mechanisms. We demonstrated that CD147 + cells from breast cancer tissue samples and cell lines possessed BCSC-like features, including the ability of self-renewal in vitro, differentiation, and tumorigenic potential in vivo. Flow cytometry analysis showed the presence of a variable fraction of CD147 + cells in 9 of 10 tumor samples. Significantly, SLC34A2 expression in CD147 + BCSCs was enhanced compared with that in differentiated adherent progeny of CD147 + BCSCs and adherently cultured cell line cells. In breast cancer patient cohorts, SLC34A2 expression was found increased in 9 of 10 tumor samples. By using lentiviral-based approach, si-SLC34A2-transduced CD147 + BCSCs showed decreased ability of sphere formation, cell viability in vitro, and tumorigenicity in vivo, which suggested the essential role of SLC34A2 in CD147 + BCSCs. Furthermore, PI3K/AKT pathway and SOX2 were found necessary to maintain the stemness of CD147 + BCSCs by using LY294002 or lentiviral-si-SOX2. Finally, we indicated that SLC34A2 could regulate SOX2 to maintain the stem cell–like features in CD147 + BCSCs through PI3K/AKT pathway. Therefore, our report identifies a novel role of SLC34A2 in BCSCs’ state regulation and establishes a rationale for targeting the SLC34A2/PI3K/AKT/SOX2 signaling pathway for breast cancer therapy.https://doi.org/10.1177/1010428317695927 |
| spellingShingle | Yonggang Lv Ting Wang Jing Fan Zhenzhen Zhang Juliang Zhang Cheng Xu Yongping Li Ge Zhao Chenyang He Huimin Meng Hua Yang Zhen Wang Jiayun Liu Jianghao Chen Ling Wang The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 breast cancer stem cells Tumor Biology |
| title | The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 breast cancer stem cells |
| title_full | The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 breast cancer stem cells |
| title_fullStr | The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 breast cancer stem cells |
| title_full_unstemmed | The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 breast cancer stem cells |
| title_short | The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147 breast cancer stem cells |
| title_sort | effects and mechanisms of slc34a2 on maintaining stem cell like phenotypes in cd147 breast cancer stem cells |
| url | https://doi.org/10.1177/1010428317695927 |
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