Genome-wide association study reveals major loci for resistance to septoria tritici blotch in a Tunisian durum wheat collection.
Septoria tritici blotch (STB) is a devastating fungal disease affecting durum and bread wheat worldwide. Tunisian durum wheat landraces are reported to be valuable genetic resources for resistance to STB and should prominently be deployed in breeding programs to develop new varieties resistant to ST...
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0310390 |
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| author | Maroua Ouaja Bikash Ghimire Bochra Amina Bahri Medini Maher Sahbi Ferjaoui Sripada Udupa Sonia Hamza |
| author_facet | Maroua Ouaja Bikash Ghimire Bochra Amina Bahri Medini Maher Sahbi Ferjaoui Sripada Udupa Sonia Hamza |
| author_sort | Maroua Ouaja |
| collection | DOAJ |
| description | Septoria tritici blotch (STB) is a devastating fungal disease affecting durum and bread wheat worldwide. Tunisian durum wheat landraces are reported to be valuable genetic resources for resistance to STB and should prominently be deployed in breeding programs to develop new varieties resistant to STB disease. In this study, a collection of 367 old durum and 6 modern wheat genotypes previously assessed using single Tunisian Zymoseptoria tritici isolate TUN06 during 2016 and 2017 and TM220 isolate during 2017 were phenotyped for resistance to a mixture of isolates (BULK) under field conditions. Significant correlations for disease traits using the three different inoculums were observed. Using 7638 SNP markers, fifty-one marker-trait associations (MTAs) for STB resistance were identified by genome-wide association study (GWAS) at Bonferroni correction threshold of -log10(P) > 5.184 with phenotypic variance explained (PVE) reaching up to 58%. A total of eleven QTL were identified using TUN06 isolate mean disease scoring (TUNMeanD and TUNMeanA) including threeQTL controlling resistance to both isolates TUN06 and TM220. A major QTL was identified on each of chromosomes 1B, 4B, 5A, and 7B, respectively. The QTL on 7B chromosome colocalized with Stb8 identified in bread wheat. Four QTL including the major QTL identified on chromosome 1B were considered as novel. SNP linked to the significant QTL have the potential to be used in marker-assisted selection for breeding for resistance to STB. |
| format | Article |
| id | doaj-art-1e6ffd60af85424fbc4a96923128d701 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-1e6ffd60af85424fbc4a96923128d7012025-02-12T05:31:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031039010.1371/journal.pone.0310390Genome-wide association study reveals major loci for resistance to septoria tritici blotch in a Tunisian durum wheat collection.Maroua OuajaBikash GhimireBochra Amina BahriMedini MaherSahbi FerjaouiSripada UdupaSonia HamzaSeptoria tritici blotch (STB) is a devastating fungal disease affecting durum and bread wheat worldwide. Tunisian durum wheat landraces are reported to be valuable genetic resources for resistance to STB and should prominently be deployed in breeding programs to develop new varieties resistant to STB disease. In this study, a collection of 367 old durum and 6 modern wheat genotypes previously assessed using single Tunisian Zymoseptoria tritici isolate TUN06 during 2016 and 2017 and TM220 isolate during 2017 were phenotyped for resistance to a mixture of isolates (BULK) under field conditions. Significant correlations for disease traits using the three different inoculums were observed. Using 7638 SNP markers, fifty-one marker-trait associations (MTAs) for STB resistance were identified by genome-wide association study (GWAS) at Bonferroni correction threshold of -log10(P) > 5.184 with phenotypic variance explained (PVE) reaching up to 58%. A total of eleven QTL were identified using TUN06 isolate mean disease scoring (TUNMeanD and TUNMeanA) including threeQTL controlling resistance to both isolates TUN06 and TM220. A major QTL was identified on each of chromosomes 1B, 4B, 5A, and 7B, respectively. The QTL on 7B chromosome colocalized with Stb8 identified in bread wheat. Four QTL including the major QTL identified on chromosome 1B were considered as novel. SNP linked to the significant QTL have the potential to be used in marker-assisted selection for breeding for resistance to STB.https://doi.org/10.1371/journal.pone.0310390 |
| spellingShingle | Maroua Ouaja Bikash Ghimire Bochra Amina Bahri Medini Maher Sahbi Ferjaoui Sripada Udupa Sonia Hamza Genome-wide association study reveals major loci for resistance to septoria tritici blotch in a Tunisian durum wheat collection. PLoS ONE |
| title | Genome-wide association study reveals major loci for resistance to septoria tritici blotch in a Tunisian durum wheat collection. |
| title_full | Genome-wide association study reveals major loci for resistance to septoria tritici blotch in a Tunisian durum wheat collection. |
| title_fullStr | Genome-wide association study reveals major loci for resistance to septoria tritici blotch in a Tunisian durum wheat collection. |
| title_full_unstemmed | Genome-wide association study reveals major loci for resistance to septoria tritici blotch in a Tunisian durum wheat collection. |
| title_short | Genome-wide association study reveals major loci for resistance to septoria tritici blotch in a Tunisian durum wheat collection. |
| title_sort | genome wide association study reveals major loci for resistance to septoria tritici blotch in a tunisian durum wheat collection |
| url | https://doi.org/10.1371/journal.pone.0310390 |
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