Genetic Diversity and Plant Growth-Promoting Activities of Root-Nodulating Bacteria in Guar Plants Across Jazan Province
Guar (<i>Cyamopsis tetragonoloba</i> L. Taub.) is a significant summer legume used as food for both humans and livestock. In Saudi Arabia, the root nodule bacteria of guar have not been studied. The present work investigated the phenotypic and genetic diversity of guar microsymbionts. Ei...
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2025-04-01
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| author | Mosbah Mahdhi Boshra Yami Mohamed Al Abboud Emad Abada Habib Khemira |
| author_facet | Mosbah Mahdhi Boshra Yami Mohamed Al Abboud Emad Abada Habib Khemira |
| author_sort | Mosbah Mahdhi |
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| description | Guar (<i>Cyamopsis tetragonoloba</i> L. Taub.) is a significant summer legume used as food for both humans and livestock. In Saudi Arabia, the root nodule bacteria of guar have not been studied. The present work investigated the phenotypic and genetic diversity of guar microsymbionts. Eighty-eight bacterial strains were isolated from the root nodules of guar grown in different locations of Jazan region of Saudi Arabia. The strains were analyzed based on their phenotypic characteristics and variations in their 16S rRNA gene sequences. A significant proportion of the isolates (90%) were fast-growing rhizobia, with 77% showing tolerance to 3–4% NaCl and 91% capable of thriving at temperatures reaching 40 °C. Several isolates exhibited strong plant growth-promoting traits, particularly in IAA production and phosphate solubilization. Genetic analysis indicated considerable diversity, with isolates classified under the genera <i>Rhizobium</i>, <i>Ensifer</i>, <i>Mesorhizobium</i>, <i>Bradyrhizobium</i>, and <i>Agrobacterium</i>. To the best of our knowledge, this study is the first to report on the phenotypic and genetic diversity of guar microsymbionts in Saudi Arabia. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Soil Systems |
| spelling | doaj-art-1e6ff952babf4f019f9dc93f17a6461e2025-08-20T03:26:52ZengMDPI AGSoil Systems2571-87892025-04-01923910.3390/soilsystems9020039Genetic Diversity and Plant Growth-Promoting Activities of Root-Nodulating Bacteria in Guar Plants Across Jazan ProvinceMosbah Mahdhi0Boshra Yami1Mohamed Al Abboud2Emad Abada3Habib Khemira4Laboratory of Biodiversity and Valorization of Bioresources in Arid Zones (LR18ES36), Faculty of Sciences of Gabes, University of Gabes, Gabes 6029, TunisiaDepartment of Biology, College of Science, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaDepartment of Biology, College of Science, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaDepartment of Biology, College of Science, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaEnvironment and Nature Research Center, Jazan University, P.O. Box 114, Jazan 45142, Saudi ArabiaGuar (<i>Cyamopsis tetragonoloba</i> L. Taub.) is a significant summer legume used as food for both humans and livestock. In Saudi Arabia, the root nodule bacteria of guar have not been studied. The present work investigated the phenotypic and genetic diversity of guar microsymbionts. Eighty-eight bacterial strains were isolated from the root nodules of guar grown in different locations of Jazan region of Saudi Arabia. The strains were analyzed based on their phenotypic characteristics and variations in their 16S rRNA gene sequences. A significant proportion of the isolates (90%) were fast-growing rhizobia, with 77% showing tolerance to 3–4% NaCl and 91% capable of thriving at temperatures reaching 40 °C. Several isolates exhibited strong plant growth-promoting traits, particularly in IAA production and phosphate solubilization. Genetic analysis indicated considerable diversity, with isolates classified under the genera <i>Rhizobium</i>, <i>Ensifer</i>, <i>Mesorhizobium</i>, <i>Bradyrhizobium</i>, and <i>Agrobacterium</i>. To the best of our knowledge, this study is the first to report on the phenotypic and genetic diversity of guar microsymbionts in Saudi Arabia.https://www.mdpi.com/2571-8789/9/2/39legumesrhizobiagenetic diversityroot nodulessymbiosis16S rRNA gene |
| spellingShingle | Mosbah Mahdhi Boshra Yami Mohamed Al Abboud Emad Abada Habib Khemira Genetic Diversity and Plant Growth-Promoting Activities of Root-Nodulating Bacteria in Guar Plants Across Jazan Province Soil Systems legumes rhizobia genetic diversity root nodules symbiosis 16S rRNA gene |
| title | Genetic Diversity and Plant Growth-Promoting Activities of Root-Nodulating Bacteria in Guar Plants Across Jazan Province |
| title_full | Genetic Diversity and Plant Growth-Promoting Activities of Root-Nodulating Bacteria in Guar Plants Across Jazan Province |
| title_fullStr | Genetic Diversity and Plant Growth-Promoting Activities of Root-Nodulating Bacteria in Guar Plants Across Jazan Province |
| title_full_unstemmed | Genetic Diversity and Plant Growth-Promoting Activities of Root-Nodulating Bacteria in Guar Plants Across Jazan Province |
| title_short | Genetic Diversity and Plant Growth-Promoting Activities of Root-Nodulating Bacteria in Guar Plants Across Jazan Province |
| title_sort | genetic diversity and plant growth promoting activities of root nodulating bacteria in guar plants across jazan province |
| topic | legumes rhizobia genetic diversity root nodules symbiosis 16S rRNA gene |
| url | https://www.mdpi.com/2571-8789/9/2/39 |
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