A constricted mitochondrial morphology formed during respiration
Abstract Mitochondria assemble in a dynamic tubular network. Their morphology is governed by mitochondrial fusion and fission, which regulate most mitochondrial functions including oxidative phosphorylation. Yet, the link between mitochondrial morphology and respiratgion remains unclear. Here, we un...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60658-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849769143175217152 |
|---|---|
| author | Manish K. Singh Laetitia Cavellini Maria Angeles Morcillo-Parra Christina Kunz Mickaël Lelek Perrine Bomme Aurélia Barascu Cynthia Alsayyah Maria Teresa Teixeira Naïma Belgareh-Touzé Adeline Mallet Lea Dietrich Christophe Zimmer Mickael M. Cohen |
| author_facet | Manish K. Singh Laetitia Cavellini Maria Angeles Morcillo-Parra Christina Kunz Mickaël Lelek Perrine Bomme Aurélia Barascu Cynthia Alsayyah Maria Teresa Teixeira Naïma Belgareh-Touzé Adeline Mallet Lea Dietrich Christophe Zimmer Mickael M. Cohen |
| author_sort | Manish K. Singh |
| collection | DOAJ |
| description | Abstract Mitochondria assemble in a dynamic tubular network. Their morphology is governed by mitochondrial fusion and fission, which regulate most mitochondrial functions including oxidative phosphorylation. Yet, the link between mitochondrial morphology and respiratgion remains unclear. Here, we uncover a mitochondrial morphology dedicated to respiratory growth of Saccharomyces cerevisiae, which we refer to as “Ringo”. The Ringo morphology is characterized by stable constrictions of mitochondrial tubules. Ringo constrictions are mediated by the yeast dynamin Dnm1 and, unlike mitochondrial fission, occur in the absence of contacts with the endoplasmic reticulum. Our data show that blocking formation of the Ringo morphology correlates with decreased respiration, decreased expression of OXPHOS subunits and perturbed mitochondrial DNA distribution. These results open important perspectives about the link between mitochondrial form and function. |
| format | Article |
| id | doaj-art-1e6fdd4d2726421884e6a5e5ce2c0a6b |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-1e6fdd4d2726421884e6a5e5ce2c0a6b2025-08-20T03:03:33ZengNature PortfolioNature Communications2041-17232025-07-0116111810.1038/s41467-025-60658-9A constricted mitochondrial morphology formed during respirationManish K. Singh0Laetitia Cavellini1Maria Angeles Morcillo-Parra2Christina Kunz3Mickaël Lelek4Perrine Bomme5Aurélia Barascu6Cynthia Alsayyah7Maria Teresa Teixeira8Naïma Belgareh-Touzé9Adeline Mallet10Lea Dietrich11Christophe Zimmer12Mickael M. Cohen13Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Sorbonne Université, CNRS, UMR8226, Institut de Biologie Physico-ChimiqueLaboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Sorbonne Université, CNRS, UMR8226, Institut de Biologie Physico-ChimiqueLaboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Sorbonne Université, CNRS, UMR8226, Institut de Biologie Physico-ChimiqueMax Planck Institute of Biophysics, Department of Structural BiologyInstitut Pasteur, Université Paris Cité, CNRS UMR 3691, Imaging and Modeling UnitUltrastructural BioImaging Core Facility, C2RT, Institut Pasteur, Université Paris CitéLaboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Sorbonne Université, CNRS, UMR8226, Institut de Biologie Physico-ChimiqueLaboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Sorbonne Université, CNRS, UMR8226, Institut de Biologie Physico-ChimiqueLaboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Sorbonne Université, CNRS, UMR8226, Institut de Biologie Physico-ChimiqueLaboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Sorbonne Université, CNRS, UMR8226, Institut de Biologie Physico-ChimiqueUltrastructural BioImaging Core Facility, C2RT, Institut Pasteur, Université Paris CitéMax Planck Institute of Biophysics, Department of Structural BiologyInstitut Pasteur, Université Paris Cité, CNRS UMR 3691, Imaging and Modeling UnitLaboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Sorbonne Université, CNRS, UMR8226, Institut de Biologie Physico-ChimiqueAbstract Mitochondria assemble in a dynamic tubular network. Their morphology is governed by mitochondrial fusion and fission, which regulate most mitochondrial functions including oxidative phosphorylation. Yet, the link between mitochondrial morphology and respiratgion remains unclear. Here, we uncover a mitochondrial morphology dedicated to respiratory growth of Saccharomyces cerevisiae, which we refer to as “Ringo”. The Ringo morphology is characterized by stable constrictions of mitochondrial tubules. Ringo constrictions are mediated by the yeast dynamin Dnm1 and, unlike mitochondrial fission, occur in the absence of contacts with the endoplasmic reticulum. Our data show that blocking formation of the Ringo morphology correlates with decreased respiration, decreased expression of OXPHOS subunits and perturbed mitochondrial DNA distribution. These results open important perspectives about the link between mitochondrial form and function.https://doi.org/10.1038/s41467-025-60658-9 |
| spellingShingle | Manish K. Singh Laetitia Cavellini Maria Angeles Morcillo-Parra Christina Kunz Mickaël Lelek Perrine Bomme Aurélia Barascu Cynthia Alsayyah Maria Teresa Teixeira Naïma Belgareh-Touzé Adeline Mallet Lea Dietrich Christophe Zimmer Mickael M. Cohen A constricted mitochondrial morphology formed during respiration Nature Communications |
| title | A constricted mitochondrial morphology formed during respiration |
| title_full | A constricted mitochondrial morphology formed during respiration |
| title_fullStr | A constricted mitochondrial morphology formed during respiration |
| title_full_unstemmed | A constricted mitochondrial morphology formed during respiration |
| title_short | A constricted mitochondrial morphology formed during respiration |
| title_sort | constricted mitochondrial morphology formed during respiration |
| url | https://doi.org/10.1038/s41467-025-60658-9 |
| work_keys_str_mv | AT manishksingh aconstrictedmitochondrialmorphologyformedduringrespiration AT laetitiacavellini aconstrictedmitochondrialmorphologyformedduringrespiration AT mariaangelesmorcilloparra aconstrictedmitochondrialmorphologyformedduringrespiration AT christinakunz aconstrictedmitochondrialmorphologyformedduringrespiration AT mickaellelek aconstrictedmitochondrialmorphologyformedduringrespiration AT perrinebomme aconstrictedmitochondrialmorphologyformedduringrespiration AT aureliabarascu aconstrictedmitochondrialmorphologyformedduringrespiration AT cynthiaalsayyah aconstrictedmitochondrialmorphologyformedduringrespiration AT mariateresateixeira aconstrictedmitochondrialmorphologyformedduringrespiration AT naimabelgarehtouze aconstrictedmitochondrialmorphologyformedduringrespiration AT adelinemallet aconstrictedmitochondrialmorphologyformedduringrespiration AT leadietrich aconstrictedmitochondrialmorphologyformedduringrespiration AT christophezimmer aconstrictedmitochondrialmorphologyformedduringrespiration AT mickaelmcohen aconstrictedmitochondrialmorphologyformedduringrespiration AT manishksingh constrictedmitochondrialmorphologyformedduringrespiration AT laetitiacavellini constrictedmitochondrialmorphologyformedduringrespiration AT mariaangelesmorcilloparra constrictedmitochondrialmorphologyformedduringrespiration AT christinakunz constrictedmitochondrialmorphologyformedduringrespiration AT mickaellelek constrictedmitochondrialmorphologyformedduringrespiration AT perrinebomme constrictedmitochondrialmorphologyformedduringrespiration AT aureliabarascu constrictedmitochondrialmorphologyformedduringrespiration AT cynthiaalsayyah constrictedmitochondrialmorphologyformedduringrespiration AT mariateresateixeira constrictedmitochondrialmorphologyformedduringrespiration AT naimabelgarehtouze constrictedmitochondrialmorphologyformedduringrespiration AT adelinemallet constrictedmitochondrialmorphologyformedduringrespiration AT leadietrich constrictedmitochondrialmorphologyformedduringrespiration AT christophezimmer constrictedmitochondrialmorphologyformedduringrespiration AT mickaelmcohen constrictedmitochondrialmorphologyformedduringrespiration |