Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer
Objective. The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression. This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesi...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/152860 |
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| author | Yvana Cristina Jorge Mayra Mioto Mataruco Leandro Pires Araújo Ana Flávia Teixeira Rossi Juliana Garcia de Oliveira Marina Curado Valsechi Alaor Caetano Kenji Miyazaki Célia Sebastiana de Jesus Fazzio Jorge Alberto Thomé Paula Rahal Sonia Maria Oliani Ana Elizabete Silva |
| author_facet | Yvana Cristina Jorge Mayra Mioto Mataruco Leandro Pires Araújo Ana Flávia Teixeira Rossi Juliana Garcia de Oliveira Marina Curado Valsechi Alaor Caetano Kenji Miyazaki Célia Sebastiana de Jesus Fazzio Jorge Alberto Thomé Paula Rahal Sonia Maria Oliani Ana Elizabete Silva |
| author_sort | Yvana Cristina Jorge |
| collection | DOAJ |
| description | Objective. The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression. This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesion as chronic gastritis (CG) and gastric adenocarcinoma (GA) and its association with H. pylori infection. Methods. We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay. Results. High ANXA1 mRNA expression levels were observed in 90% (36/40) of CG cases (mean relative quantification RQ = 4.26 ± 2.03) and in 80% (16/20) of GA cases (mean RQ = 4.38 ± 4.77). However, LGALS1 mRNA levels were high (mean RQ = 2.44 ± 3.26) in 60% (12/20) of the GA cases, while low expression was found in CG (mean RQ = 0.43±3.13; P<0.01). Normal mucosa showed modest immunoreactivity in stroma but not in epithelium, while stroma and epithelium displayed an intense immunostaining in CG and GA for both proteins. Conclusion. These results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis. |
| format | Article |
| id | doaj-art-1e6f445564234ea186932b91c65dda7b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-1e6f445564234ea186932b91c65dda7b2025-02-03T01:24:55ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/152860152860Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric CancerYvana Cristina Jorge0Mayra Mioto Mataruco1Leandro Pires Araújo2Ana Flávia Teixeira Rossi3Juliana Garcia de Oliveira4Marina Curado Valsechi5Alaor Caetano6Kenji Miyazaki7Célia Sebastiana de Jesus Fazzio8Jorge Alberto Thomé9Paula Rahal10Sonia Maria Oliani11Ana Elizabete Silva12Department of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, BrazilDepartment of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, BrazilDepartment of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, BrazilDepartment of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, BrazilDepartment of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, BrazilDepartment of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, BrazilRio Preto Endoscopy Center, São José do Rio Preto, SP, BrazilEndoscopy Service, Hospital de Base, São José do Rio Preto, SP, BrazilLegal Medicine Department and Pathology Service, Hospital de Base, São José do Rio Preto, SP, BrazilInstitute of Anatomical Pathology and Cytopathology, São José do Rio Preto, SP, BrazilDepartment of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, BrazilDepartment of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, BrazilDepartment of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, BrazilObjective. The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression. This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesion as chronic gastritis (CG) and gastric adenocarcinoma (GA) and its association with H. pylori infection. Methods. We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay. Results. High ANXA1 mRNA expression levels were observed in 90% (36/40) of CG cases (mean relative quantification RQ = 4.26 ± 2.03) and in 80% (16/20) of GA cases (mean RQ = 4.38 ± 4.77). However, LGALS1 mRNA levels were high (mean RQ = 2.44 ± 3.26) in 60% (12/20) of the GA cases, while low expression was found in CG (mean RQ = 0.43±3.13; P<0.01). Normal mucosa showed modest immunoreactivity in stroma but not in epithelium, while stroma and epithelium displayed an intense immunostaining in CG and GA for both proteins. Conclusion. These results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis.http://dx.doi.org/10.1155/2013/152860 |
| spellingShingle | Yvana Cristina Jorge Mayra Mioto Mataruco Leandro Pires Araújo Ana Flávia Teixeira Rossi Juliana Garcia de Oliveira Marina Curado Valsechi Alaor Caetano Kenji Miyazaki Célia Sebastiana de Jesus Fazzio Jorge Alberto Thomé Paula Rahal Sonia Maria Oliani Ana Elizabete Silva Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer Mediators of Inflammation |
| title | Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer |
| title_full | Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer |
| title_fullStr | Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer |
| title_full_unstemmed | Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer |
| title_short | Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer |
| title_sort | expression of annexin a1 and galectin 1 anti inflammatory proteins and mrna in chronic gastritis and gastric cancer |
| url | http://dx.doi.org/10.1155/2013/152860 |
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