Overexpression of CsBRC, an F‐box gene from Camellia sinensis, increased the plant branching in tobacco and rice
Abstract Tea plant (Camellia sinensis [L.]) is one of the most important crops in China, and tea branch is an important agronomic trait that determines the yield of tea plant. In previous work focused on GWAS that detecting GWAS signals related to plant architecture through whole genome re‐sequencin...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-07-01
|
| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.618 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841558626633777152 |
|---|---|
| author | Bokun Zhou Qi Sheng Xinzhuan Yao Tong Li Litang Lu |
| author_facet | Bokun Zhou Qi Sheng Xinzhuan Yao Tong Li Litang Lu |
| author_sort | Bokun Zhou |
| collection | DOAJ |
| description | Abstract Tea plant (Camellia sinensis [L.]) is one of the most important crops in China, and tea branch is an important agronomic trait that determines the yield of tea plant. In previous work focused on GWAS that detecting GWAS signals related to plant architecture through whole genome re‐sequencing of ancient tea plants, a gene locus TEA 029928 significantly related to plant type was found. Sequence alignment results showed that this gene belonged to the F‐box family. We named it CsBRC. CsBRC‐GFP fusion proteins were mainly localized in the plasma membrane. By comparing the phenotypes of CsBRC transgenic tobacco and WT tobacco, it was found that the number of branches of transgenic tobacco was significantly higher than that of wild‐type tobacco. Through RNA‐seq analysis, it was found that CsBRC affects the branching development of plants by regulating the expression of genes related to brassinosteroid synthesis pathway in plants. In addition, overexpression of CsBRC in rice could increase tiller number, grain length and width, and 1,000‐grain weight. |
| format | Article |
| id | doaj-art-1e526e0afd4042c69a4bec0840ae8349 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-1e526e0afd4042c69a4bec0840ae83492025-01-06T07:21:54ZengWileyPlant Direct2475-44552024-07-0187n/an/a10.1002/pld3.618Overexpression of CsBRC, an F‐box gene from Camellia sinensis, increased the plant branching in tobacco and riceBokun Zhou0Qi Sheng1Xinzhuan Yao2Tong Li3Litang Lu4Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Science Guizhou University Guiyang ChinaKey Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Science Guizhou University Guiyang ChinaCollege of Tea Science, Institute of Plant Health and Medicine Guizhou University Guiyang ChinaCollege of Tea Science, Institute of Plant Health and Medicine Guizhou University Guiyang ChinaKey Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Science Guizhou University Guiyang ChinaAbstract Tea plant (Camellia sinensis [L.]) is one of the most important crops in China, and tea branch is an important agronomic trait that determines the yield of tea plant. In previous work focused on GWAS that detecting GWAS signals related to plant architecture through whole genome re‐sequencing of ancient tea plants, a gene locus TEA 029928 significantly related to plant type was found. Sequence alignment results showed that this gene belonged to the F‐box family. We named it CsBRC. CsBRC‐GFP fusion proteins were mainly localized in the plasma membrane. By comparing the phenotypes of CsBRC transgenic tobacco and WT tobacco, it was found that the number of branches of transgenic tobacco was significantly higher than that of wild‐type tobacco. Through RNA‐seq analysis, it was found that CsBRC affects the branching development of plants by regulating the expression of genes related to brassinosteroid synthesis pathway in plants. In addition, overexpression of CsBRC in rice could increase tiller number, grain length and width, and 1,000‐grain weight.https://doi.org/10.1002/pld3.618F‐box proteinphenotypic analysisRNA‐seqtea tree |
| spellingShingle | Bokun Zhou Qi Sheng Xinzhuan Yao Tong Li Litang Lu Overexpression of CsBRC, an F‐box gene from Camellia sinensis, increased the plant branching in tobacco and rice Plant Direct F‐box protein phenotypic analysis RNA‐seq tea tree |
| title | Overexpression of CsBRC, an F‐box gene from Camellia sinensis, increased the plant branching in tobacco and rice |
| title_full | Overexpression of CsBRC, an F‐box gene from Camellia sinensis, increased the plant branching in tobacco and rice |
| title_fullStr | Overexpression of CsBRC, an F‐box gene from Camellia sinensis, increased the plant branching in tobacco and rice |
| title_full_unstemmed | Overexpression of CsBRC, an F‐box gene from Camellia sinensis, increased the plant branching in tobacco and rice |
| title_short | Overexpression of CsBRC, an F‐box gene from Camellia sinensis, increased the plant branching in tobacco and rice |
| title_sort | overexpression of csbrc an f box gene from camellia sinensis increased the plant branching in tobacco and rice |
| topic | F‐box protein phenotypic analysis RNA‐seq tea tree |
| url | https://doi.org/10.1002/pld3.618 |
| work_keys_str_mv | AT bokunzhou overexpressionofcsbrcanfboxgenefromcamelliasinensisincreasedtheplantbranchingintobaccoandrice AT qisheng overexpressionofcsbrcanfboxgenefromcamelliasinensisincreasedtheplantbranchingintobaccoandrice AT xinzhuanyao overexpressionofcsbrcanfboxgenefromcamelliasinensisincreasedtheplantbranchingintobaccoandrice AT tongli overexpressionofcsbrcanfboxgenefromcamelliasinensisincreasedtheplantbranchingintobaccoandrice AT litanglu overexpressionofcsbrcanfboxgenefromcamelliasinensisincreasedtheplantbranchingintobaccoandrice |