Reduction of Total Phenols in Virgin Olive Oil as a Preservation Medium during Cold Storage of Whey Cheese and Tofu

Research background. Virgin olive oil, known as a good source of health-promoting hydrophilic phenols, is traditionally used as a medium for preserving various foods. Phenols in general can form complexes with proteins, but there is little information on the direct contact of virgin olive oil with p...

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Main Authors: Valerija Majetić Germek, Ivana Gobin, Doris Franjković, Marija Marković, Olivera Koprivnjak
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2024-01-01
Series:Food Technology and Biotechnology
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Online Access:https://hrcak.srce.hr/file/465279
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author Valerija Majetić Germek
Ivana Gobin
Doris Franjković
Marija Marković
Olivera Koprivnjak
author_facet Valerija Majetić Germek
Ivana Gobin
Doris Franjković
Marija Marković
Olivera Koprivnjak
author_sort Valerija Majetić Germek
collection DOAJ
description Research background. Virgin olive oil, known as a good source of health-promoting hydrophilic phenols, is traditionally used as a medium for preserving various foods. Phenols in general can form complexes with proteins, but there is little information on the direct contact of virgin olive oil with protein-rich food during long-term storage. In this study, the dynamics of the decline of total phenols in oil used as preservation medium for a traditional (whey cheese skuta) and a modern product (tofu) were compared. Experimental approach. Pieces of skuta or tofu immersed in virgin olive oil at different food-to-oil mass ratios were stored in the refrigerator for up to 21 days. The oil quality indices, water content and the total count of aerobic mesophilic bacteria in the immersed materials were monitored. To determine the total phenols in the oil, the optimal conditions of the Fast Blue BB test, which is a suitable alternative to the standard method, were tested and selected. Results and conclusions. The effect of both materials on the indicators of hydrolytic and oxidative deterioration of the oil is almost identical (a gradual decrease), which is most likely due to the continuous release of water from the immersed food. A sharp decrease in total phenols in the oil (by about 50 %) after seven days of storage in contact with both materials indicates a combination of causes (water-to-oil migration and phenol-protein interactions). The form of the rational function is highly representative of the decrease in total phenols during the first seven days of tofu/oil storage, indicating a very rapid interaction with tofu proteins. The preservative effect of virgin olive oil in terms of microbiological spoilage was not observed. Novelty and scientific contribution. The results of this study contribute to the knowledge on the dynamics of phenol-protein interactions and emphasise the need for further investigations on traditional or newly used protein-rich foods preserved in direct contact with virgin olive oil, taking into account possible changes in the functional, nutritional and sensory properties of phenols and proteins.
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spelling doaj-art-1e4dca6e341a4f27a681cda977bf56722025-08-20T01:47:18ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062024-01-0162334635310.17113/ftb.62.03.24.8434Reduction of Total Phenols in Virgin Olive Oil as a Preservation Medium during Cold Storage of Whey Cheese and TofuValerija Majetić Germek0Ivana Gobin1Doris Franjković2Marija Marković3Olivera Koprivnjak4University of Rijeka, Faculty of Medicine, Department of Food Technology and Quality Control, Braće Branchetta 20, 51000 Rijeka, CroatiaUniversity of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchetta 20, 51000 Rijeka, CroatiaUniversity of Rijeka, Faculty of Medicine, Braće Branchetta 20, 51000 Rijeka, CroatiaUniversity of Rijeka, Faculty of Medicine, Braće Branchetta 20, 51000 Rijeka, CroatiaUniversity of Rijeka, Faculty of Medicine, Department of Food Technology and Quality Control, Braće Branchetta 20, 51000 Rijeka, CroatiaResearch background. Virgin olive oil, known as a good source of health-promoting hydrophilic phenols, is traditionally used as a medium for preserving various foods. Phenols in general can form complexes with proteins, but there is little information on the direct contact of virgin olive oil with protein-rich food during long-term storage. In this study, the dynamics of the decline of total phenols in oil used as preservation medium for a traditional (whey cheese skuta) and a modern product (tofu) were compared. Experimental approach. Pieces of skuta or tofu immersed in virgin olive oil at different food-to-oil mass ratios were stored in the refrigerator for up to 21 days. The oil quality indices, water content and the total count of aerobic mesophilic bacteria in the immersed materials were monitored. To determine the total phenols in the oil, the optimal conditions of the Fast Blue BB test, which is a suitable alternative to the standard method, were tested and selected. Results and conclusions. The effect of both materials on the indicators of hydrolytic and oxidative deterioration of the oil is almost identical (a gradual decrease), which is most likely due to the continuous release of water from the immersed food. A sharp decrease in total phenols in the oil (by about 50 %) after seven days of storage in contact with both materials indicates a combination of causes (water-to-oil migration and phenol-protein interactions). The form of the rational function is highly representative of the decrease in total phenols during the first seven days of tofu/oil storage, indicating a very rapid interaction with tofu proteins. The preservative effect of virgin olive oil in terms of microbiological spoilage was not observed. Novelty and scientific contribution. The results of this study contribute to the knowledge on the dynamics of phenol-protein interactions and emphasise the need for further investigations on traditional or newly used protein-rich foods preserved in direct contact with virgin olive oil, taking into account possible changes in the functional, nutritional and sensory properties of phenols and proteins.https://hrcak.srce.hr/file/465279virgin olive oilwhey cheesetofutotal phenolsFast Blue BB test
spellingShingle Valerija Majetić Germek
Ivana Gobin
Doris Franjković
Marija Marković
Olivera Koprivnjak
Reduction of Total Phenols in Virgin Olive Oil as a Preservation Medium during Cold Storage of Whey Cheese and Tofu
Food Technology and Biotechnology
virgin olive oil
whey cheese
tofu
total phenols
Fast Blue BB test
title Reduction of Total Phenols in Virgin Olive Oil as a Preservation Medium during Cold Storage of Whey Cheese and Tofu
title_full Reduction of Total Phenols in Virgin Olive Oil as a Preservation Medium during Cold Storage of Whey Cheese and Tofu
title_fullStr Reduction of Total Phenols in Virgin Olive Oil as a Preservation Medium during Cold Storage of Whey Cheese and Tofu
title_full_unstemmed Reduction of Total Phenols in Virgin Olive Oil as a Preservation Medium during Cold Storage of Whey Cheese and Tofu
title_short Reduction of Total Phenols in Virgin Olive Oil as a Preservation Medium during Cold Storage of Whey Cheese and Tofu
title_sort reduction of total phenols in virgin olive oil as a preservation medium during cold storage of whey cheese and tofu
topic virgin olive oil
whey cheese
tofu
total phenols
Fast Blue BB test
url https://hrcak.srce.hr/file/465279
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AT dorisfranjkovic reductionoftotalphenolsinvirginoliveoilasapreservationmediumduringcoldstorageofwheycheeseandtofu
AT marijamarkovic reductionoftotalphenolsinvirginoliveoilasapreservationmediumduringcoldstorageofwheycheeseandtofu
AT oliverakoprivnjak reductionoftotalphenolsinvirginoliveoilasapreservationmediumduringcoldstorageofwheycheeseandtofu