Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicum
The four enzymes of acetate kinase (AK), phosphotransacetylase (PTA), isocitrate lyase (ICL) and malate systhase (MS) which are involved in the acetate metabolism of Corynebacterium glutamicum have been developed in their assay methods. The enzyme activities of AK, PTA, ICL and MS are analyzed compa...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2003-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.05.0529 |
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| author | RUAN Hong XUAN Ri-rong |
| author_facet | RUAN Hong XUAN Ri-rong |
| author_sort | RUAN Hong |
| collection | DOAJ |
| description | The four enzymes of acetate kinase (AK), phosphotransacetylase (PTA), isocitrate lyase (ICL) and malate systhase (MS) which are involved in the acetate metabolism of Corynebacterium glutamicum have been developed in their assay methods. The enzyme activities of AK, PTA, ICL and MS are analyzed comparatively while C. glutamicum grows on glucose or/and on acetate. The results showed there was “glucose effect” in carbon metabolism and acetate could significantly promote the induction of specific activities of acetate kinase, phosphotransacetylase, isocitrate lyase and malate systhase. |
| format | Article |
| id | doaj-art-1e43f2c7e8f748cc891d191e37ba2bf6 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2003-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-1e43f2c7e8f748cc891d191e37ba2bf62025-08-20T02:54:50ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552003-09-012952953310.3785/1008-9209.2003.05.052910089209Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicumRUAN HongXUAN Ri-rongThe four enzymes of acetate kinase (AK), phosphotransacetylase (PTA), isocitrate lyase (ICL) and malate systhase (MS) which are involved in the acetate metabolism of Corynebacterium glutamicum have been developed in their assay methods. The enzyme activities of AK, PTA, ICL and MS are analyzed comparatively while C. glutamicum grows on glucose or/and on acetate. The results showed there was “glucose effect” in carbon metabolism and acetate could significantly promote the induction of specific activities of acetate kinase, phosphotransacetylase, isocitrate lyase and malate systhase.https://www.academax.com/doi/10.3785/1008-9209.2003.05.0529<italic>Corynebacterium glutamicum</italic>acetate metabolismphosphotransacetylase (PTA)acetate kinase (AK)isocitrate lyase (ICL)malate systhase (MS) |
| spellingShingle | RUAN Hong XUAN Ri-rong Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicum 浙江大学学报. 农业与生命科学版 <italic>Corynebacterium glutamicum</italic> acetate metabolism phosphotransacetylase (PTA) acetate kinase (AK) isocitrate lyase (ICL) malate systhase (MS) |
| title | Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicum |
| title_full | Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicum |
| title_fullStr | Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicum |
| title_full_unstemmed | Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicum |
| title_short | Studies on activities of PTA, AK, ICL and MS enzymes involved in the acetate metabolism of Corynebacterium glutamicum |
| title_sort | studies on activities of pta ak icl and ms enzymes involved in the acetate metabolism of corynebacterium glutamicum |
| topic | <italic>Corynebacterium glutamicum</italic> acetate metabolism phosphotransacetylase (PTA) acetate kinase (AK) isocitrate lyase (ICL) malate systhase (MS) |
| url | https://www.academax.com/doi/10.3785/1008-9209.2003.05.0529 |
| work_keys_str_mv | AT ruanhong studiesonactivitiesofptaakiclandmsenzymesinvolvedintheacetatemetabolismofcorynebacteriumglutamicum AT xuanrirong studiesonactivitiesofptaakiclandmsenzymesinvolvedintheacetatemetabolismofcorynebacteriumglutamicum |