Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications
This work describes the fabrication, optimization, and characterization of electrospun fibrous poly(D,L-lactic acid) (PDLLA) for biomedical applications. The influences of the polymer concentration of the electrospinning solution (5, 10, or 15 wt%) and the solution flow rate (0.1, 0.5, 1.0, or 2.0 m...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Advances in Materials Science and Engineering |
| Online Access: | http://dx.doi.org/10.1155/2013/140643 |
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| author | Toshihiro Inami Yasuhiro Tanimoto Masayuki Ueda Yo Shibata Satoshi Hirayama Masaru Yamaguchi Kazutaka Kasai |
| author_facet | Toshihiro Inami Yasuhiro Tanimoto Masayuki Ueda Yo Shibata Satoshi Hirayama Masaru Yamaguchi Kazutaka Kasai |
| author_sort | Toshihiro Inami |
| collection | DOAJ |
| description | This work describes the fabrication, optimization, and characterization of electrospun fibrous poly(D,L-lactic acid) (PDLLA) for biomedical applications. The influences of the polymer concentration of the electrospinning solution (5, 10, or 15 wt%) and the solution flow rate (0.1, 0.5, 1.0, or 2.0 mL/h) on the morphology of the obtained fibrous PDLLA were evaluated. The in vitro biocompatibility of two types of PDLLA, ester terminated PDLLA (PDLLA-R) and carboxyl terminated PDLLA (PDLLA-COOH), was evaluated by monitoring apatite formation on samples immersed in Hanks’ balanced salt (HBS) solution. 15 wt% polymer solution was the most beneficial for preparing a fibrous PDLLA structure. Meanwhile, no differences in morphology were observed for PDLLA prepared at various flow rates. Apatite precipitate is formed on both types of PDLLA only 1 day after immersion in HBS solution. After 7 days of immersion, PDLLA-COOH showed greater apatite formation ability compared with that of PDLLA-R, as measured by thin-film X-ray diffraction. The results indicated that the carboxyl group is effective for apatite precipitation in the body environment. |
| format | Article |
| id | doaj-art-1e426990e6ec4bdcaaf592fa6bab9531 |
| institution | Kabale University |
| issn | 1687-8434 1687-8442 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Materials Science and Engineering |
| spelling | doaj-art-1e426990e6ec4bdcaaf592fa6bab95312025-02-03T05:59:02ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422013-01-01201310.1155/2013/140643140643Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical ApplicationsToshihiro Inami0Yasuhiro Tanimoto1Masayuki Ueda2Yo Shibata3Satoshi Hirayama4Masaru Yamaguchi5Kazutaka Kasai6Department of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, JapanDepartment of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, JapanDepartment of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, JapanDepartment of Oral Biomaterials and Technology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, JapanDepartment of Dental Caries and Aesthetic Dentistry, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, JapanDepartment of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, JapanDepartment of Orthodontics, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho Nishi, Matsudo, Chiba 271-8587, JapanThis work describes the fabrication, optimization, and characterization of electrospun fibrous poly(D,L-lactic acid) (PDLLA) for biomedical applications. The influences of the polymer concentration of the electrospinning solution (5, 10, or 15 wt%) and the solution flow rate (0.1, 0.5, 1.0, or 2.0 mL/h) on the morphology of the obtained fibrous PDLLA were evaluated. The in vitro biocompatibility of two types of PDLLA, ester terminated PDLLA (PDLLA-R) and carboxyl terminated PDLLA (PDLLA-COOH), was evaluated by monitoring apatite formation on samples immersed in Hanks’ balanced salt (HBS) solution. 15 wt% polymer solution was the most beneficial for preparing a fibrous PDLLA structure. Meanwhile, no differences in morphology were observed for PDLLA prepared at various flow rates. Apatite precipitate is formed on both types of PDLLA only 1 day after immersion in HBS solution. After 7 days of immersion, PDLLA-COOH showed greater apatite formation ability compared with that of PDLLA-R, as measured by thin-film X-ray diffraction. The results indicated that the carboxyl group is effective for apatite precipitation in the body environment.http://dx.doi.org/10.1155/2013/140643 |
| spellingShingle | Toshihiro Inami Yasuhiro Tanimoto Masayuki Ueda Yo Shibata Satoshi Hirayama Masaru Yamaguchi Kazutaka Kasai Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications Advances in Materials Science and Engineering |
| title | Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications |
| title_full | Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications |
| title_fullStr | Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications |
| title_full_unstemmed | Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications |
| title_short | Morphology and In Vitro Behavior of Electrospun Fibrous Poly(D,L-lactic acid) for Biomedical Applications |
| title_sort | morphology and in vitro behavior of electrospun fibrous poly d l lactic acid for biomedical applications |
| url | http://dx.doi.org/10.1155/2013/140643 |
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